[논문]Leptin potentiates Prevotella intermedia lipopolysaccharide-induced production of TNF-<tex>$\\alpha$</tex> in monocyte-derived macrophages

Kim, Sung-Jo

doi:10.5051/jpis.2010.40.3.119

Leptin potentiates Prevotella intermedia lipopolysaccharide-induced production of TNF-$\\alpha$ in monocyte-derived macrophages 원문보기

Journal of periodontal & implant science, v.40 no.3, 2010년, pp.119 - 124

Kim, Sung-Jo (Department of Periodontology, Pusan National University College of Dentistry)

Abstract ▼ AI-Helper

Purpose: In addition to regulating body weight, leptin is also recognized for its role in the regulation of immune function and inflammation. The purpose of this study was to investigate the effect of leptin on Prevotella (P.) intermedia lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-$\alpha$ production in differentiated THP-1 cells, a human monocytic cell line. Methods: LPS from P. intermedia ATCC 25611 was prepared by the standard hot phenol-water method. THP-1 cells were incubated in the medium supplemented with phorbol myristate acetate to induce differentiation into macrophage-like cells. The amount of TNF-$\alpha$ and interleukin-8 secreted into the culture medium was determined by enzyme-linked immunosorbent assay (ELISA). TNF-$\alpha$ and Ob-R mRNA expression levels were determined by semi-quantitative reverse transcription-polymerase chain reaction analysis. Results: Leptin enhanced P. intermedia LPS-induced TNF-$\alpha$ production in a dose-dependent manner. Leptin modulated P. intermedia LPS-induced TNF-$\alpha$ expression predominantly at the transcriptional level. Effect of leptin on P. intermedia LPS-induced TNF-$\alpha$ production was not mediated by the leptin receptor. Conclusions: The ability of leptin to enhance P. intermedia LPS-induced TNF-$\alpha$ production may be important in the establishment of chronic lesion accompanied by osseous tissue destruction observed in inflammatory periodontal disease.

주제어

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문제 정의

The presence of leptin and Ob-R in oral mucosa and salivary glands suggests that the activity of leptin is also of signifi cance to diseases affecting the oral cavity. The purpose of this study was to investigate the effect of leptin on P. intermedia LPS-induced TNF-a production in differentiated THP-1 cells, a human monocytic cell line.
intermedia LPS might be related to an increase in Ob-R expression, Ob-R mRNA expression levels were determined by semi-quantitative RT-PCR analysis. This study demonstrated the presence of the long and short leptin receptors in differentiated THP-1 cells. However, leptin recep tors were not modulated by P.

제안 방법

Total RNA was isolat ed with an RNeasy Mini Kit (Qiagen, Valencia, USA), accord ing to the manufacturer's instructions. Synthesis of cDNA from the extracted RNA and subsequent amplification of the cDNA by RT-PCR were carried out with an AccuPower RT/ PCR Premix kit (Bioneer, Seoul, Korea) and thermal cycler (GeneAmp PCR system 2400, PE Applied Biosystems, Foster City, USA). p-actin served as internal control.
To verify whether the Ob-R is responsible for mediating the synergistic effect of leptin on P. intermedia LPS-induced TNF-a production in differentiated THP-1 cells, Ob-R mRNA expression levels were determined by semi-quantitative RT-PCR analysis. The long (Ob-Rb) and short (Ob-Ra) leptin re ceptors were expressed in untreated and leptin-treated cells, while upregulation of Ob-R by P.
To verify whether the mechanism behind the synergistic effect of leptin with P. intermedia LPS might be related to an increase in Ob-R expression, Ob-R mRNA expression levels were determined by semi-quantitative RT-PCR analysis. This study demonstrated the presence of the long and short leptin receptors in differentiated THP-1 cells.

데이터처리

Statistical analysis was performed using Student's paired t-test with P<0.05 considered statistically significant. Data are expressed as means±SD of three independent experiments.

성능/효과

The results of this study indicate that leptin potentiates P. intermedia LPS-induced TNF-a production in a dose-depen dent manner. Leptin modulated P.

참고문헌 (34)

1 Williams RC Periodontal disease N Engl J Med 1990 322 373 382 2405268

상세보기
2 Slots J Bragd L Wikstrom M Dahlen G The occurrence of Actinobacillus actinomycetemcomitans, Bacteroides gingivalis and Bacteroides intermedius in destructive periodontal disease in adults J Clin Periodontol 1986 13 570 577 3462204

상세보기
3 Chung CP Nisengard RJ Slots J Genco RJ Bacterial IgG and IgM antibody titers in acute necrotizing ulcerative gingivitis J Periodontol 1983 54 557 562 6138399

상세보기
4 Kornman KS Loesche WJ The subgingival microbial flora during pregnancy J Periodontal Res 1980 15 111 122 6103927

상세보기
5 Morrison DC Ryan JL Endotoxins and disease mechanisms Annu Rev Med 1987 38 417 432 3555304
6 Zhang Y Proenca R Maffei M Barone M Leopold L Friedman JM Positional cloning of the mouse obese gene and its human homologue Nature 1994 372 425 432 7984236

상세보기
7 Maffei M Fei H Lee GH Dani C Leroy P Zhang Y Increased expression in adipocytes of ob RNA in mice with lesions of the hypothalamus and with mutations at the db locus Proc Natl Acad Sci U S A 1995 92 6957 6960 7624352

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8 Flier JS The adipocyte: storage depot or node on the energy information superhighway? Cell 1995 80 15 18 7813011

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9 Bouloumie A Drexler HC Lafontan M Busse R Leptin, the product of Ob gene, promotes angiogenesis Circ Res 1998 83 1059 1066 9815153

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10 Martin-Romero C Santos-Alvarez J Goberna R Sanchez-Margalet V Human leptin enhances activation and proliferation of human circulating T lymphocytes Cell Immunol 2000 199 15 24 10675271

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11 Masuzaki H Ogawa Y Sagawa N Hosoda K Matsumoto T Mise H Nonadipose tissue production of leptin: leptin as a novel placenta-derived hormone in humans Nat Med 1997 3 1029 1033 9288733

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12 Lord GM Matarese G Howard JK Baker RJ Bloom SR Lechler RI Leptin modulates the T-cell immune response and reverses starvation-induced immunosuppression Nature 1998 394 897 901 9732873

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13 Janik JE Curti BD Considine RV Rager HC Powers GC Alvord WG Interleukin 1 alpha increases serum leptin concentrations in humans J Clin Endocrinol Metab 1997 82 3084 3086 9284748
14 Sanchez-Margalet V Martin-Romero C Human leptin signaling in human peripheral blood mononuclear cells: activation of the JAK-STAT pathway Cell Immunol 2001 211 30 36 11585385

상세보기
15 Halaas JL Friedman JM Leptin and its receptor J Endocrinol 1997 155 215 216 9415051

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16 Hill RA Margetic S Pegg GG Gazzola C Leptin: its pharmacokinetics and tissue distribution Int J Obes Relat Metab Disord 1998 22 765 770 9725636

상세보기
17 Breidert M Miehlke S Glasow A Orban Z Stolte M Ehninger G Leptin and its receptor in normal human gastric mucosa and in Helicobacter pylori-associated gastritis Scand J Gastroenterol 1999 34 954 961 10563663
18 Groschl M Rauh M Wagner R Neuhuber W Metzler M Tamguney G Identification of leptin in human saliva J Clin Endocrinol Metab 2001 86 5234 5239 11701683

상세보기
19 Westphal O Jann K Whistler RL Bacterial lipopolysaccharides: extraction with phenol-water and further applications of the procedure Methods in carbohydrate chemistry. General polysaccharides 1965 Vol. 5 New York Academic Press 83 91
20 Markwell MA Haas SM Bieber LL Tolbert NE A modification of the Lowry procedure to simplify protein determination in membrane and lipoprotein samples Anal Biochem 1978 87 206 210 98070

상세보기
21 Birkedal-Hansen H Role of cytokines and inflammatory mediators in tissue destruction J Periodontal Res 1993 28 500 510 8263720

상세보기
22 Stoufi ED Taubman MA Ebersole JL Smith DJ Stashenko PP Phenotypic analyses of mononuclear cells recovered from healthy and diseased human periodontal tissues J Clin Immunol 1987 7 235 245 3496355

상세보기
23 Arnalich F Lopez J Codoceo R Jim nez M Madero R Montiel C Relationship of plasma leptin to plasma cytokines and human survivalin sepsis and septic shock J Infect Dis 1999 180 908 911 10438392

상세보기
24 Finck BN Johnson RW Tumor necrosis factor (TNF)-alpha induces leptin production through the p55 TNF receptor Am J Physiol Regul Integr Comp Physiol 2000 278 R537 R543 10666158
25 Faggioni R Fantuzzi G Fuller J Dinarello CA Feingold KR Grunfeld C IL-1 beta mediates leptin induction during inflammation Am J Physiol 1998 274 R204 R208 9458919
26 Ahima RS Flier JS Leptin Annu Rev Physiol 2000 62 413 437 10845097

상세보기
27 Karthikeyan BV Pradeep AR Gingival crevicular fluid and serum leptin: their relationship to periodontal health and disease J Clin Periodontol 2007 34 467 472 17451417

상세보기
28 Karthikeyan BV Pradeep AR Leptin levels in gingival crevicular fluid in periodontal health and disease J Periodontal Res 2007 42 300 304 17559625

상세보기
29 Johnson RB Serio FG Leptin within healthy and diseased human gingiva J Periodontol 2001 72 1254 1257 11577959

상세보기
30 Rossomando EF Kennedy JE Hadjimichael J Tumour necrosis factor alpha in gingival crevicular fluid as a possible indicator of periodontal disease in humans Arch Oral Biol 1990 35 431 434 2196868

상세보기
31 Stashenko P Jandinski JJ Fujiyoshi P Rynar J Socransky SS Tissue levels of bone resorptive cytokines in periodontal disease J Periodontol 1991 62 504 509 1920018

상세보기
32 Abu-Amer Y Ross FP Edwards J Teitelbaum SL Lipopolysaccharide-stimulated osteoclastogenesis is mediated by tumor necrosis factor via its P55 receptor J Clin Invest 1997 100 1557 1565 9294124

상세보기
33 Kobayashi K Takahashi N Jimi E Udagawa N Takami M Kotake S Tumor necrosis factor alpha stimulates osteoclast differentiation by a mechanism independent of the ODF/RANKL-RANK interaction J Exp Med 2000 191 275 286 10637272
34 Assuma R Oates T Cochran D Amar S Graves DT IL-1 and TNF antagonists inhibit the inflammatory response and bone loss in experimental periodontitis J Immunol 1998 160 403 409 9551997

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DOI : 10.5051/jpis.2010.40.3.119 [무료]
대한치주과학회 : 저널 > 논문 [무료]
PubMed Central : 저널 > 논문 [무료]
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