[논문]토사자(菟絲子)의 추출용매에 따른 항염, 항산화 및 항균 효과에 대한 비교 연구

황보민; 서형식

doi:10.3831/kpi.2011.14.1.079

토사자(菟絲子)의 추출용매에 따른 항염, 항산화 및 항균 효과에 대한 비교 연구
The Comparative Study of Anti-inflammatory, Antioxidant and Antibacterial Effects with Regard to the Extraction Solvents of Cuscutae Semen 원문보기

Journal of pharmacopuncture, v.14 no.1, 2011년, pp.79 - 86

황보민 (부산대학교 한의학전문대학원 안이비인후피부과) , 서형식 (부산대학교 한의학전문대학원 안이비인후피부과)

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Objective : This study was performed to compare anti-inflammation, anti-oxidation and anti-bacterial effects of Cuscutae Semen(CS) extracted with two kinds of solvents, ethanol and distilled water. Methods : Two kinds of CS extractions were prepared 20, 50, 100 ${\mu}l/mg$. The cytotoxicity was measured by MTT assay in Raw 264.7 cell. The anti-inflammation effect was measured by inhibitory efficacy of NO Production in Raw 264.7 cell. The anti-oxidation effect was measured by DPPH Radical scavenging ability in HaCaT cell. The anti-bacterial effect was measured by inhibition zone diameter on Propionibacterium acnes. Results : 1. Two kinds(100 ${\mu}l/mg$) of CS extraction groups had 50% cytotoxicity in Raw 264.7 cell. 2. All of CS extraction groups were not showed significantly inhibitory effect on NO production. 3. All of CS extracted with ethanol only showed dose-dependently significantly scavenging effect of DPPH radicals. 4. Two kinds of CS extractions did not have a inhibitory effect on Propionibactrium acnes. Conclusion : Two kinds(100 ${\mu}l/mg$) of CS extraction groups have 50% cytotoxicity. Two kinds of CS extractions have not the inhibitory effect on NO production and Propionibactrium acnes. CS groups extracted with ethanol only have a significantly scavenging ability of DPPH radicals. This study suggests that CS extracted with ethanol was effective in anti-oxidation.

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참고문헌 (22)

Pang TS, Lee KG, Ham IH, Bu YG, Kim HC, Rhee JS, Choi HY. A Study on the Content Changes of ${\beta}$ -asarone and ${\alpha}$ -asarone in Acorus gramineus According to its Parts, Extraction Solvent, and Fermentation. Kor. J. Herbology. 2008;23(4):149-57.
Leem KH, Kim HC, Ahn KD. A Study for Standardization of Zingiberis Rhizoma - A Study on Qurntitative Analysis of 6-gingerol in Zingiberis officinale Roscoe by HPLC and extracting rate with different eluants -. J. of Herbology. 11(2);1996:85-91.
許浚. 東醫寶鑑. 서울:法仁文化社. 1999:518.
H. Kim, H. Lim, B. Kim, H. Kim, S. Choi, C. Yoon, Studies on the anti-acne effect of Agrimonia pilosa Ledeb. J. Soc. Cosmet. Scientists Korea. 2006;32(1):53-8.
Kwak HS, Lee DH, Moon HS, Park JS, Woo KJ, Kim CM. Antimicrobial effect of Bamboo (Phyllosrachys Bambusoides) Essential Oil on Trichophyton and Pityrosporum. J. Fd Hyg. Safety. 2003;18(3):118-24.
T. Mosmann. Rapid colorimetric assay for cellular growth and survival application of proliferation and cytotoxicity. J. Immunol. Methods. 1983;65:55.

상세보기
Han SM, Lee KG, Yeao JH, Kweon HY, Park KK, Anti-inflammatory effect of the venom from asian honeybees (Apis cerana L.) on inducible nitric oxide synthase and tumor necrosis factor- {\alpha}$ in Raw 264.7 cell line. Korean J. Apiculture. 2004;19:89.
M. S. Blois. Antioxidant determinanations by the use of a stable free radical, Nature. 1958;181:1199.

상세보기
Lee SE, Son DW, Yoon YP, Lim TY, Kim HS. Screening of Traditional Herbral Medicines to Develop New Materials for Anti-aging and Anti-wrinkle in the skin. Soc. Cosmet. Scientists Korea. 2005;31(2):147-52.
Yu HJ, Choi IH. A Practical Application on the External Treatment of Acne (A Focus on the Medical Herbs Published in the Woman's Monthly Magazines). The Journal of Oriental Medical Ophthalmology & Otolaryngology & Dermatology 2004;17(1):34-44.
顧伯康. 中醫外科學. 北京:人民衛生出版社. 1987:60.
Du IS, Seo YJ, Woo WH, Oh HC, Park MC, Hwang CY, Lim KS, Kim NK. Clinical Study on the Effect of External Treatment of 5% Herba Houttuyniae Extract Solution on the Acne. Korean journal of oriental physiology & pathology. 2004;18(2):612-20.
Hong SH. The Clinical Study on the Effect of Jeondo-san(Diandao-san) on Acnes. Journal of Korean oriental medical society. 2005;26(3):74-9.
Lee SY, Seo HS. The Effects of Sulfur extract on Anti-Inflammation and Anti-Propionibacterium acnes. The journal of Korean oriental medical ophthalmology & otolaryngology & dermatology. 2007;20(2):68-76.
Yoo JG, Seo HS. The Effects of Yeouigeumhwang-san on Anti-Inflammation and Anti-Propionibacterium acnes. The journal of Korean oriental medical ophthalmology & otolaryngology & dermatology. 2007;20(2):77-88.
Choi KH, Seo HS. The Effects of Jeondo-san on Anti-Inflammation and Anti-Propionibacterium acnes. The journal of Korean oriental medical ophthalmology & otolaryngology & dermatology. 2007;20(2):89-101.
Kim MA, Kim MB, Shin SH, Byun SM, Ko WS, Lee HS, Park SG, Yoon HJ. The study on external treatment of oriental medical care on acne. The journal of Korean oriental medical ophthalmology & otolaryngology & dermatology. 2008;21(2):102-11.
The Korean Society of Pathologitis. Pathology. Seoul:Komoonsa. 2000:34,43.
Seo HS. The Comparative Study of Anti-inflammation and Anti-oxidation in Accodance with Extraction Solvents of Jeondo-san. The journal of Korean oriental medical ophthalmology & otolaryngology & dermatology. 2010;23(2):69-80.
Kim H, Lee MJ, Lee HS, Jung HJ, Choi SK, Lee CH, Park WH. Preventive Effects of GLEDITSIAE SPINA Ethanol Extracts and its Fraction in Oxidative Stress and Human LDL Oxidation. Korean J. Oriental Physiology & Pathology. 2009;23(3):631-8.
Seo HS. The experimental study on anti-inflammation and anti-oxidation of Indigo Naturalis and Rehmammiae Radix. The journal of Korean oriental medical ophthalmology & otolaryngology & dermatology. 2008;21(3):104-10.
Choi KH, Seo HS. The effects of Jeondo-san on anti-inflammation and anti-Propionibacterium acnes. The journal of Korean oriental medical ophthalmology & otolaryngology & dermatology. 2007;20(2):89-101.

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토사자(菟絲子)의 추출용매에 따른 항염, 항산화 및 항균 효과에 대한 비교 연구
The Comparative Study of Anti-inflammatory, Antioxidant and Antibacterial Effects with Regard to the Extraction Solvents of Cuscutae Semen 원문보기

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토사자(菟絲子)의 추출용매에 따른 항염, 항산화 및 항균 효과에 대한 비교 연구 The Comparative Study of Anti-inflammatory, Antioxidant and Antibacterial Effects with Regard to the Extraction Solvents of Cuscutae Semen 원문보기

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토사자(菟絲子)의 추출용매에 따른 항염, 항산화 및 항균 효과에 대한 비교 연구
The Comparative Study of Anti-inflammatory, Antioxidant and Antibacterial Effects with Regard to the Extraction Solvents of Cuscutae Semen 원문보기