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논문 상세정보

도라지 부탄올 추출물의 항산화 및 nitric oxide 생성 저해 효과

Inhibitory Effect of Extracts of Platycodon grandiflorum (the Ballon Flower) on Oxidation and Nitric Oxide Production

초록

본 연구에서는 도라지 분말을 유기용매로 추출한 후 도라지의 추출물과 분획물들에 의한 세포 내 활성산소종 및 glutathione (GSH)를 측정하여 항산화효과를 검토하였고 NO 생성 저해 효과를 알아보았다. 세포 내 활성산소종 생성억제 실험에서 건조 도라지의 A+M 및 MeOH 추출물과 추출물을 n-hexane, 85% aq. MeOH, n-BuOH, water로 다시 추출하여 얻어진 각각의 분획물들을 농도별로 HT1080 세포에 처리하였을 때 A+M과 MeOH 추출물 모두 측정시간 120분 동안 $500\;{\mu}M$ $H_2O_2$만을 처리한 control군에 비해 세포 내 활성산소종을 크게 억제시켰으며, MeOH 추출물에 의한 항산화 효과가 더 높게 나타났다. 또한, 각 분획물들 중 n-BuOH 분획물이 다른 분획물들에 비해 우수한 항산화 활성을 보였다. GSH 농도 측정 실험에서 A+M 및 85% aq. MeOH 분획물를 처리했을 때 GSH 함량이 증가하였다. NO 생성 저해 실험에서는 A+M 및 MeOH 추출물이 0.01 및 0.05 mg/mL의 농도에서 NO 생성을 억제하는 것으로 나타났으며, A+M 추출물에 의한 저해 효과가 높았다. 각 분획물들은 모두 control보다 낮은 NO 생성량을 나타내었으며, 특히 85% aq. MeOH 및 n-BuOH 분획물은 0.05 mg/mL 농도에서 blank에 가까운 NO 생성 억제율을 나타냈다. 이상의 연구결과로부터 n-BuOH 분획물에 의한 세포 내 활성산소종 생성 억제 효과 및 NO 생성 저해 효과가 우수함을 알 수 있었으며, 향후 분획물의 분리 정제를 통한 새로운 기능성 물질의 개발이 필요할 것으로 사료된다.

Abstract

We explored the effect of extracts of dried Platycodon grandiflorum on production of reactive oxygen species (ROS), glutathione (GSH) and nitric oxide (NO). To determine antioxidant activity in the presence of $H_2O_2$-induced oxidative stress, DCFH-DA (dichlorodihydrofluorescin diacetate) assay was employed. Acetone/methylene chloride (A+M) and methanolic (MeOH) extracts of P. grandiflorum reduced intracellular ROS levels. Of the various tested fractions, n-BuOH fraction showed the highest protective effect in terms of lipid peroxide production. Total GSH levels were measured after treatment of HT1080 cells with the A+M and MeOH extracts, and other solvent fractions, at various concentration. The A+M extacts and 85% (v/v) aqueous MeOH fraction significantly increased GSH levels (p<0.05). When lipopolysaccharide (LPS)-induced NO production was evaluated, all tested crude extracts, and fractions thereof, significantly reduced NO production (p<0.05), and the n-BuOH and 85% (v/v) aqueous MeOH fractions (at 0.05 mg/mL) showed the strongest inhibitory effects. The results showed that the n-BuOH fraction inhibited both cellular oxidation and NO production, and this fraction may thus contain valuable active compounds.

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  1. Kim, Geum-Soog ; Park, Chun-Geun ; Lee, Kyeong-Hee ; Choi, Jehun ; Lee, Seung-Eun ; Noh, Hyung-Jun ; Lee, Jeong-Hoon ; Kim, Seung-Yu 2011. "Investigation of Shikonin Pigments and Antioxidant activity of the Roots from Lithospermum erythrorhizon according to the Different Growth Stages and Areas of Cultivation" 韓國藥用作物學會誌 = Korean journal of medicinal crop science, 19(6): 435~440 
  2. Kwon, Hoo-Ja ; Park, Chan-Sung 2011. "Development and Quality Characteristics of Bellflower Root Jeonggwa Added Omija (Schizandra chinensis Baillon) during Storage" 한국식품저장유통학회지 = Korean journal of food preservation, 18(3): 279~287 
  3. Kim, Hye-Rim ; Gim, Seon-Bin ; Yun, Mi-Young ; Lee, Ki-Moo ; Kim, Dong-Hee 2012. "Effect of CST on atopic dermatitis related inflammatory cytokines" 論文集 : 大田大學校 韓醫學硏究所. 韓醫學編, 20(2): 41~52 
  4. Jung, Kyung-Ae ; Park, Chan-Sung 2012. "Physiological Activities of Fermented Garlic Broth during Fermentation" 한국식품저장유통학회지 = Korean journal of food preservation, 19(3): 406~412 
  5. Hwang, Seong Yeon ; Choi, Hyang Mi ; Lim, Sun-Young 2013. "Total Phenolics of Dried Platycodon grandiflorum and Its Effect on Growth of Human Cancer Cell Lines" 한국식품과학회지 = Korean journal of food science and technology, 45(1): 84~89 
  6. Ko, Yu-Jin ; Seol, Hui-Gyeong ; Lee, Gyeong-Ran ; Jeong, Gye-Im ; Ryu, Chung-Ho 2013. "Anti-inflammatory Effect and Antioxidative Activities of Ingredients used in Bibimbab" 생명과학회지 = Journal of life science, 23(2): 213~221 
  7. Jeong, Seung-Il ; Yu, Hyeon Hee 2013. "Quality Characteristics of Sikhe Prepared with the Roots Powder of Doraji (Platycodon grandiflorum A. DE. Candolle)" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 42(5): 759~765 
  8. Lee, Soo-Jin ; Shin, Seung-Ryeul ; Yoon, Kyung-Young 2013. "Physicochemical Properties of Black Doraji (Platycodon grandiflorum)" 한국식품과학회지 = Korean journal of food science and technology, 45(4): 422~427 
  9. Lee, Sang Hoon ; Song, Eun Mi ; Jang, Gwi Yeong ; Li, Meishan ; Kim, Min Young ; Park, Hye Jin ; Kang, Tae Su ; Jeong, Heon Sang 2013. "Physicochemical Characteristics and Antioxidant Activities of Doragi (Platycodon grandiflorum) at Different Aging Temperatures and for Various Durations" 한국식품영양과학회지 = Journal of the Korean Society of Food Science and Nutrition, 42(9): 1405~1411 
  10. Kim, Jung 2014. "Antibacterial and anti-inflammatory effects of Platycodon grandiflorum extracts" 디지털융복합연구 = Journal of digital convergence, 12(3): 359~366 
  11. Bae, Man-Jong ; Kim, Soo-Jung ; Cho, Min-Seog ; Um, Yong-Bin ; Bae, Myung-In 2014. "Fermentation characteristic of Yeongdeok Bobsikhae to which a natural substance (Bellflower) was added" 한국식품저장유통학회지 = Korean journal of food preservation, 21(3): 350~356 

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