N-(p-Coumaroyl)serotonin과 N-Feruloylserotonin은 홍화씨에서 유래하는 특이적인 세로토닌유도체로 주로 식물이 병원균을 방어하기 위해서 만들어내는 hydroxycinnamic acid amides계 물질이다. 본 연구에서는 N-(p-Coumaroyl) serotonin과 N-Feruloylserotonin이 지방전구세포의 지방분화에 미치는 영향을 oil-red O염색과 triglyceride 양 측정, GPDH 활성 측정 등을 통해 알아보았고, 그 결과 두 물질 모두 유의적으로 지방세포분화를 억제함을 관찰하였다. 효능 비교물질로 세로토닌을 처리했을 때 세로토닌 자체로는 지방분화에 유의미한 효과는 관찰되지 않았다. N-(p-Coumaroyl) serotonin과 N-Feruloylserotonin은 또한 우수한 항산화능을 보여주었다. 이러한 결과를 통해, N-(p-Coumaroyl)serotonin과 N-Feruloylserotonin이 지방세포분화억제를 통한 항비만 소재로서의 가능성을 확인하였다.
N-(p-Coumaroyl)serotonin과 N-Feruloylserotonin은 홍화씨에서 유래하는 특이적인 세로토닌유도체로 주로 식물이 병원균을 방어하기 위해서 만들어내는 hydroxycinnamic acid amides계 물질이다. 본 연구에서는 N-(p-Coumaroyl) serotonin과 N-Feruloylserotonin이 지방전구세포의 지방분화에 미치는 영향을 oil-red O염색과 triglyceride 양 측정, GPDH 활성 측정 등을 통해 알아보았고, 그 결과 두 물질 모두 유의적으로 지방세포분화를 억제함을 관찰하였다. 효능 비교물질로 세로토닌을 처리했을 때 세로토닌 자체로는 지방분화에 유의미한 효과는 관찰되지 않았다. N-(p-Coumaroyl) serotonin과 N-Feruloylserotonin은 또한 우수한 항산화능을 보여주었다. 이러한 결과를 통해, N-(p-Coumaroyl)serotonin과 N-Feruloylserotonin이 지방세포분화억제를 통한 항비만 소재로서의 가능성을 확인하였다.
N-feruloylserotonin (FS) and N-(pcoumaroyl) serotonin (CS), serotonin derivatives, which have been isolated as major and unique phenolics of safflower seed extract (SSE), are member of hydroxycinnamic acid amides and are implicated in the defense against pathogen infection and insect feeding. In thi...
N-feruloylserotonin (FS) and N-(pcoumaroyl) serotonin (CS), serotonin derivatives, which have been isolated as major and unique phenolics of safflower seed extract (SSE), are member of hydroxycinnamic acid amides and are implicated in the defense against pathogen infection and insect feeding. In this study, we evaluate inhibitory effects of N-(p-Coumaroyl)serotonin and N-Feruloylserotonin on adipogenesis using oil-red O staining, triglyceride and GPDH activity. we found that while serotonin itself did not suppress differentiation of preadipocytes into adipocytes, N-(p-Coumaroyl)serotonin and N-Feruloylserotonin inhibited the differentiation of preadipocytes into adipocytes in a concentration-dependent manner. In addition, they showed antioxidant effects in DPPH assay. Taken together, these results show that N-feruloylserotonin (FS) and N-(pcoumaroyl) serotonin (CS) suppress differentiation of preadipocytes, suggesting the possibility that these serotonin derivatives can be utilized as an anti-obesity agent.
N-feruloylserotonin (FS) and N-(pcoumaroyl) serotonin (CS), serotonin derivatives, which have been isolated as major and unique phenolics of safflower seed extract (SSE), are member of hydroxycinnamic acid amides and are implicated in the defense against pathogen infection and insect feeding. In this study, we evaluate inhibitory effects of N-(p-Coumaroyl)serotonin and N-Feruloylserotonin on adipogenesis using oil-red O staining, triglyceride and GPDH activity. we found that while serotonin itself did not suppress differentiation of preadipocytes into adipocytes, N-(p-Coumaroyl)serotonin and N-Feruloylserotonin inhibited the differentiation of preadipocytes into adipocytes in a concentration-dependent manner. In addition, they showed antioxidant effects in DPPH assay. Taken together, these results show that N-feruloylserotonin (FS) and N-(pcoumaroyl) serotonin (CS) suppress differentiation of preadipocytes, suggesting the possibility that these serotonin derivatives can be utilized as an anti-obesity agent.
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제안 방법
Until now, no study has investigated the effect of serotonin derivatives on differentiation of preadipocytes. In this study, we investigated the inhibitory effects of serotonin derivatives on adipogenesis by measuring oil-red O staining, triglyceride and GPDH activity in 3T3-L1.
To further characterize the effects of N-feruloylserotonin (FS) and N-(pcoumaroyl) serotonin (CS) on differentiation, cellular triglyceride content and GPDH enzyme activity were measured. By the time of full differentiation at day 9, cells treated with N-feruloylserotonin (FS) and N-(pcoumaroyl) serotonin (CS) inhibited triglyceride accumulation compared with the controls (Figure 3).
Two-day postconfluent 3T3-L1 preadipocytes (day 0) were treated with the indicated concentrations of serotonin derivatives every 2 days for 9 days. No significant effect on cell viability has been observed with serotonin derivatives concentrations under 75 μM (Figure 2A).
대상 데이터
Serotonin, isobutylmethylxanthine, dexamethasone, and insulin were purchased from Sigma–Aldrich (St. Louis, MO, USA).
데이터처리
The statistical significance of the data was determined by Student’s t-test.
성능/효과
In conclusion, N-feruloylserotonin (FS) and N-(pcoumaroyl) serotonin (CS) have anti-adipogenic and antioxidant activities, which are beneficial for preventing obesity.
참고문헌 (20)
M. J. Devlin, S. Z. Yanovski, and G. T. Wilson, Obesity: what mental health professionals need to know, Am. J. Psychiatry, 157, 854 (2000).
K. Fujioka, Management of obesity as a chronic disease: nonpharmacologic, pharmacologic, and surgical options, Obes. Res., 10, 116S (2002).
S. MJitrapakdee, G. Slawik, M. Medina-Gomez, J. C. Campbell, J. K. Wallace, S. Sethi, O'Rahilly, and A. J. Vidal-Puig, The peroxisome proliferator- activated receptor-gamma regulates murine pyruvate carboxylase gene expression in vivo, and in vitro, J. Biol. Chem., 280, 27466 (2005).
Z. Cao, R. M. Umek, and S. L. McKnight, Regulated expression of three C/EBP isoforms during adipose conversion of 3T3-L1 cells, Genes Dev., 5, 1538 (1991).
P. Tontonoz, J. B. Kim, R. A. Graves, and B. M. Spiegelman, ADD1: a novel helix-loop-helix transcription factor associated with adipocyte determination and differentiation, Mol. Cell. Biol., 13, 4753 (1993).
C. L. Hsu and G. C. Yen, Effects of capsaicin on induction of apoptosis and inhibition of adipogenesis in 3T3-L1 cells, J. Agric. Food. Chem., 55, 1730 (2007).
H. K. Kim, C. Nelson-Dooley, M. A. Della-Fera, J. Y. Yang, W. Zhang, and D. L. Hartzell, Genistein decreases food intake, body weight and fat pad weight and causes adipose tissue apoptosis in ovariectomized female mice, J Nutr., 136, 409 (2006).
C. Huang, Y. Zhang, Z. Gong, X. Sheng, Z. Li, and W. Zhang, Berberine inhibits 3T3-L1 adipocyte differentiation through the PPARgamma pathway, Biochem. Biophys. Res. Commun., 348, 571 (2006).
J. M. Brown, M. S. Boysen, S. S. Jensen, R. F. Morrison, J. Storkson, and R. L. Currie, Isomer-specific regulation of metabolism and PPAR $\gamma$ signaling by CLA in human preadipocytes, J. Lipid. Res., 44, 1287 (2003).
M. H. Cha, I. C. Kim, B. H. Lee, and Y. Yoon, Baicalein inhibits adipocyte differentiation by enhancing COX-2 expression, J. Med. Food, 9, 145 (2006).
H. S. Moon, H. G. Lee, Y. J. Choi, T. G. Kim, and C. S. Cho. Proposed mechanisms of (-)-epigallocatechin- 3-gallate for anti-obesity, Chem. Biol. Interact., 167(2), 85 (2007).
H. L. Zhang, A. Nagatsu, T. Watanabe, J. Sakakibara, and H. Okuyama, Antioxidative compoundsisolated from safflower (Carthamus tinctorius L.) oil cake, Chem. Pharm. Bull., 45, 1910 (1997).
S. H. Cho, H. R. Lee, T. H. Kim, S. W. Choi, W. J. Lee, and Y. Choi, Effects of defatted safflower seed extract and phenolic compounds in diet on plasma and liver lipid in ovariectomized rats fed high-cholesterol diets, J. Nutr. Sci. Vitaminol., 50, 32 (2004).
N. Koyama, K. Kuribayashi, T. Seki, K. Kobayashi, Y. Furuhata, K. Suzuki, H. Arisaka, and T. Nakano, Serotonin derivatives, major safflower (Carthamus tinctorius L.) seed antioxidants, inhibit low-density lipoprotein (LDL) oxidation and atherosclerosis in apolipoprotein E-deficient mice, J. Agric. Food. Chem., 54, 4970 (2006).
Y. Hotta, A. Nagatsu, W. Liu, T. Muto, C. Narumiya, X. Lu, M. Yajima, N. Ishikawa, K. Miyazeki, N. Kawai, H. Mizukami, and J. Sakakibara, Protective effects of antioxidative serotonin derivatives isolated from safflower against ostischemic myocardial dysfunction, Mol. Cell. Biochem., 238, 151 (2002).
J. S. Roh, J. Y. Han, J. H. Kim, and J. K. Hwang, Inhibitory effects of active compounds isolated from safflower (Carthamus tinctorius L.) seeds for melanogenesis, Biol. Pharm. Bull., 27(12), 1976 (2004).
S. M. Jang, A. Ishihara, and K. Back, Production of coumaroylserotonin and feruloylserotonin in transgenic rice expressing pepper hydroxycinnamoylcoenzyme A: serotonin N-(hydroxycinnamoyl) transferase, Plant. Physiol., 135(1), 346 (2004).
L. P. Kozak and J. T. Jensen, Genetic and developmental control of multiple forms of L-glycerol 3-phosphate dehydrogenase, J. Biol. Chem., 249(24), 7775 (1974).
J. C. Halford, J. A. Harrold, E. J. Boyland, C. Lawton, and J. E. Blundell, Serotonergic drugs : effects on appetite expression and use for the treatment of obesity, Drugs, 67(1), 27 (2007).
S. Furukawa, T. Fujita, M. Shimabukuro, M. Iwaki, Y. Yamada, Y. Nakajima, O. Nakayama, M. Makishima, M. Matsuda, and I. Shimomura, Increased oxidative stress in obesity and its impact on metabolic syndrome, J. Clin. Invest., 114(12), 1752 (2004).
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