RAPD마커를 이용한 국내골프장의 잔디 13 품종의 유전적 다양성 분석 Analysis of Genetic Diversity in Thirteen Turfgrass Cultivars Cultivated at Golf Courses Using RAPD Markers원문보기
본 연구는 무작위 분자마커(RAPD)를 이용한 우리나라 골프장에서 이용되고 있는 잔디 13개 잔디품종의 유전적 다형성을 조사하여 보다 효과적인 골프장 관리를 위한 유전적 정보를 제공하고자 조사하였다. 본 연구에서 사용한 54개의 random hexamer primer를 이용하여 RAPD분석을 실시한 결과 13~54개의 다형성 밴드를 형성하였으며 primer당 평균 30.7개의 다형성 밴드를 확인할 수 있었다. RAPD분석 결과 13개의 잔디품종은 크게 3개의 그룹으로 나눌 수 있었다. Group I은 Shadow II, Aurora Gold, Little Big Horn Blue, PennA-1, PennA-4, Group II는 Midnight II, Prosperity, Moonlight SLT, Bright Star SLT, Silver Dollar, Group III은 Olympic Gold Turf-Type, Silver Star Turf-Type, Tar Heel II Turf-Type을 포함하였다. 13개 잔디 품종의 유전적 근연 정도는 0.039~1.0으로 나타났으며, Group III이 유전적 근연 정도가 가장 높게 나타났다. 이상의 결과를 통해 향후, 잔디 종 또는 이종간의 유전적 다양성의 상호관계나 차이점을 규명하기 위해서는 형태적인 특성과 SCARs 마커와 같은 특이적인 분자마커에 대한 연구가 추가적으로 필요할 것으로 사료된다.
본 연구는 무작위 분자마커(RAPD)를 이용한 우리나라 골프장에서 이용되고 있는 잔디 13개 잔디품종의 유전적 다형성을 조사하여 보다 효과적인 골프장 관리를 위한 유전적 정보를 제공하고자 조사하였다. 본 연구에서 사용한 54개의 random hexamer primer를 이용하여 RAPD분석을 실시한 결과 13~54개의 다형성 밴드를 형성하였으며 primer당 평균 30.7개의 다형성 밴드를 확인할 수 있었다. RAPD분석 결과 13개의 잔디품종은 크게 3개의 그룹으로 나눌 수 있었다. Group I은 Shadow II, Aurora Gold, Little Big Horn Blue, PennA-1, PennA-4, Group II는 Midnight II, Prosperity, Moonlight SLT, Bright Star SLT, Silver Dollar, Group III은 Olympic Gold Turf-Type, Silver Star Turf-Type, Tar Heel II Turf-Type을 포함하였다. 13개 잔디 품종의 유전적 근연 정도는 0.039~1.0으로 나타났으며, Group III이 유전적 근연 정도가 가장 높게 나타났다. 이상의 결과를 통해 향후, 잔디 종 또는 이종간의 유전적 다양성의 상호관계나 차이점을 규명하기 위해서는 형태적인 특성과 SCARs 마커와 같은 특이적인 분자마커에 대한 연구가 추가적으로 필요할 것으로 사료된다.
This study was carried our to examine the genetic relationship of 13 commercial turfgrass cultivars using Random Amplified Polymorphic DNA to provide genetic informations more efficient golf course management. Analysis of 56 random hexamer primers generated 13 to 54 polymorphic bands among the 13 cu...
This study was carried our to examine the genetic relationship of 13 commercial turfgrass cultivars using Random Amplified Polymorphic DNA to provide genetic informations more efficient golf course management. Analysis of 56 random hexamer primers generated 13 to 54 polymorphic bands among the 13 cultivars with an average of 30.7 bands per primer. The results of cluster analysis based on RAPDs revealed that three major variety groups: Group I - 'Shadow II', 'Aurora Gold', 'Little Bighorn Blue', 'PennA-1', and 'PennA-4'; Group II - 'Midnight II', 'Prosperity', 'Moon light SLT', 'Bright star SLT', and 'Silver dollar'; and Group III - 'Olympic Gold', 'Silver Star', and 'Tar Heel II'. The genetic similarity coefficients among 13 turfgrass cultivars ranged from 0.039 to 1.0 with highest coefficient in Group III. Studies on morphological characters and the effective molecular markers such as sequence characterized amplified regions are further needed to identify relationships and genetic diversities within species and among species.
This study was carried our to examine the genetic relationship of 13 commercial turfgrass cultivars using Random Amplified Polymorphic DNA to provide genetic informations more efficient golf course management. Analysis of 56 random hexamer primers generated 13 to 54 polymorphic bands among the 13 cultivars with an average of 30.7 bands per primer. The results of cluster analysis based on RAPDs revealed that three major variety groups: Group I - 'Shadow II', 'Aurora Gold', 'Little Bighorn Blue', 'PennA-1', and 'PennA-4'; Group II - 'Midnight II', 'Prosperity', 'Moon light SLT', 'Bright star SLT', and 'Silver dollar'; and Group III - 'Olympic Gold', 'Silver Star', and 'Tar Heel II'. The genetic similarity coefficients among 13 turfgrass cultivars ranged from 0.039 to 1.0 with highest coefficient in Group III. Studies on morphological characters and the effective molecular markers such as sequence characterized amplified regions are further needed to identify relationships and genetic diversities within species and among species.
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문제 정의
This study analyzed the genetic diversity of the commercial turfgrass cultivars used as mono and hybridization components on golf courses in Korea to provide basic information on developing turfgrass cultivars.
This study revealed the genetic diversity of 13 turfgrass cultivars commercially used on the golf courses in Korea. Turfgrass cultivars most widely used on the golf courses in Korea were effectively classified through morphological characterization.
제안 방법
1. Polymorphism patterns with random primers P127 (A), P128 (B), P129 (C), P130 (D), P131 (E), and P133 (F) to identify genetic differences for 13 turfgrass cultivars. M lane: Molecular marker (100 bp of ladder DNA plus); lane 1: Shadow II; lane 2: ‘PennA-1’; lane 3: ‘PennA-4’; lane 4: ‘Aurora Gold’; lane 5: ‘Little Big Horn Blue’; lane 6: ‘Midnight II’; lane 7: ‘Moonlight SLT’; lane 8: ‘Prosperity’; lane 9: ‘Bright Star SLT’; lane 10: ‘Silver Dollar’; lane 11: ‘Olympic Gold’; lane 12: ‘Silver Star’ and lane 13: ‘Tar Heel II’.
This matrix was analyzed by the “Power Marker” (http://statgen.ncsu.edu/powermarker/) software to construct a dendrogram and principal coordinate analysis.
대상 데이터
, Korea). A total of 56 random hexamer primers were selected for assessing RAPD analysis of 13 turfgrass cultivars (Table 2). Polymorphic DNA bands were amplified with Bioneer premix kit (Bioneer Co.
데이터처리
Sequential, agglomerative, hierarchical, and nested clustering was carried out with unweighted pairgroup method and arithmetic average (UPGMA) method. A simple matching coefficient was used to calculate the similarity coefficient matrix in NTSYS-pc.
3. The genetic dendrogram of 13 turfgrass cultivars was constructed from similarity coefficients and showing the clustering of the unweighted pair group average linkage of Pearson coefficients. SHII: ‘Shadow II’; PeA1: ‘PennA-1’; PeA4: ‘PennA-4’; AuGo: ‘Aurora Gold’; LBB: ‘Little Big Horn Blue; MiII: ‘Midnight II’; MSLT: ‘Moonlight SLT’; Pro: ‘Prosperity’; BSLT: ‘Bright Star SLT’; Silv: ‘Silver Dollar’; OGTF: ‘Olympic Gold’ ; STT: ‘Silver Star’ and HIITT: ‘Tar Heel II’.
Table 4. The genetic similarity matrix among 13 turfgrass cultivars used on golf courses in Korea based on Pearson coefficient (r).
이론/모형
Similarity matrix was calculated using SIMQUAL. Sequential, agglomerative, hierarchical, and nested clustering was carried out with unweighted pairgroup method and arithmetic average (UPGMA) method. A simple matching coefficient was used to calculate the similarity coefficient matrix in NTSYS-pc.
0, Numerical Taxonomy and Multivariate Analysis System) software. Similarity matrix was calculated using SIMQUAL. Sequential, agglomerative, hierarchical, and nested clustering was carried out with unweighted pairgroup method and arithmetic average (UPGMA) method.
edu/powermarker/) software to construct a dendrogram and principal coordinate analysis. Using this similarity coefficient matrix, genetic distance was analyzed by a UPGMA method.
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