Biological Application of Two Protozoan Species, Euplotes sp. and Vorticella sp., for the Stable Culture of the Rotifer Brachionus rotundiformis in Laboratory Experiments of Inter- and Tripartite-Specific Relations
Members of the ciliate group of protozoans are often observed in mass cultures of rotifers. In particular, Euplotes and Vorticella are common contaminating species. In this study, I examined the effect of the ciliates Euplotes sp. and Vorticella sp. on the growth of the rotifer Brachionus rotundiformis by conducting inter-specific and tripartite-specific mixed-culture experiments. The growth of rotifers was suppressed in co-existence with Euplotes sp. compared with monocultures of rotifers. However, Vorticella sp. promoted rotifer growth. Moreover, Vorticella sp. improved the growth of rotifers suppressed by Euplotes sp. contaminants. In 5-L semi-mass cultures of rotifers, growth of the contaminating protozoan Euplotes sp. was heavily suppressed by Vorticella sp. The stable maintenance of the rotifer culture ecosystem can be achieved by manipulating the types of contaminating protozoan species.
Gilbert JJ and Jack JD. 1993. Rotifers as predators on small ciliates. Hydrobiologia 255/256, 247-253.
Hagiwara A, Hamada K, Hori S and Hirayama K. 1994. Increased sexual reproduction in Brachionus plicatilis (Rotifera) with the addition of bacteria and rotifer extracts. J Exp Mar Biol Ecol 181, 1-8.
Hagiwara A, Jung MM, Sato T and Hirayama K. 1995. Interspecific relations between marine rotifer Brachionus rotundiformis and zooplankton species contaminating in the rotifer mass culture tank. Fish Sci 61, 623-627.
Hamilton RD and Preslan JE. 1969. Cultural characteristics of a pelagic marine Hymenostome ciliate, Uronema sp. J Exp Mar Biol Ecol 4, 90-99.
Inoki S. 1981. An Illustrated of the Protozoa. Kodansha Publishing, Tokyo, JP.
Jung MM, Hagiwara A and Hirayama K. 1997. Interspecific interactions in the marine rotifer microcosm. Hydrobiologia 358, 121-126.
Jung MM, Moon TS, Kim HS and Ji YJ. 2008. Role of contaminant a ciliate Euplotes (Ciliate, Protozoa) in the rotifer Brachionus rotundiformis culture tanks. J Aquac 21, 167-171.
Kawabata Z, Matsui K, Okazaki K, Nasu M, Nakano N and Sugai T. 1995. Synthesis of a species-defined microcosm with protozoa. J Protozool Res 5, 23-26.
Maeda M and Hino A. 1991. Environmental management for mass culture of the rotifer, Brachionus plicatilis. Rotifer and microalgae culture systems. In: Proceeding of a U.S.-Asia Workshop. Fulls W and Main KL, eds. The Oceanic Institute, Honolulu, HI, US, pp. 125-133.
Pace ML and Orcutt JD. 1981. The relative importance of protozoans, rotifers, and crustaceans in a freshwater zooplankton community. Limnol Oceanogr 26, 822-830.
Pierce RW and Turner JT. 1992. Ecology of planktonic ciliates in marine food webs. Rev Aquat Sci 6, 139-181.
Reguera B. 1984. The effect of ciliate contamination in mass cultures of the rotifer, Brachionus plicatilis O. F. Muller. Aquaculture 40, 103-108.