[논문]발모효과를 가지는 한방복합처방단

이준영; 임경란; 정택규; 이명희; 윤경섭

doi:10.15230/scsk.2012.38.4.277

발모효과를 가지는 한방복합처방단
Medicinal Herbal Complex Extract with Potential for Hair Growth-Promoting Activity 원문보기

大韓化粧品學會誌 = Journal of the society of cosmetic scientists of Korea, v.38 no.4, 2012년, pp.277 - 287

이준영 ((주)사임당화장품 기술연구소) , 임경란 ((주)사임당화장품 기술연구소) , 정택규 ((주)사임당화장품 기술연구소) , 이명희 (대전보건대학교 화장품과학과) , 윤경섭 ((주)사임당화장품 기술연구소)

초록
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탈모방지 및 발모효과를 가지는 소재를 개발하기 위해 한의학에서 전통적으로 사용되는 23가지 한방소재를 선정하여 한방복합처방단을 개발하였다. 한방복합처방단을 구성하는 한방소재들은 예로부터 전통적으로 발모 및 탈모방지, 흰머리방지, 염증 치료 및 혈액순환개선 효과를 가진 것으로 알려져 있는 당귀, 보골지, 측백엽, 한련초, 구기자, 복분자, 상백피, 숙지황, 여정실, 적하수오, 흑지마, 고삼, 백지, 익모초, 단삼, 도인, 몰약, 감국, 유향, 인삼, 천궁, 합환피, 현호색 등이다. 또한, 한방복합처방단의 발모효과를 확인하기 위해 in vitro와 in vivo 평가모델을 이용하여 모발성장 및 촉진에 미치는 영향을 실험하였다. In vitro 상에서는 모유두세포, 각질형성세포 및 섬유아세포의 증식을 확인하였다. 또한, 흰머리방지 효과와 관련하여 멜라노마 세포에서의 멜라닌 합성능력을 확인하였다. 한방복합처방단의 in vitro 상에서의 육모효과는 C57BL/6 마우스를 이용한 in vivo 상에서도 확인하였다. 연구 결과 한방복합처방단은 $50{\mu}g/mL$의 농도에서 모유두 세포의 증식을 175 %까지, 섬유아세포인 NIH3T3 세포의 증식은 120 %까지 증가시켰으며, $20{\mu}g/mL$의 농도에서 각질형성세포인 HaCaT 세포의 증식을 133 %까지 증가시켰다. 멜라닌 합성의 경우, $50{\mu}g/mL$의 농도에서 154 %까지 증가시켰다. 또한, C57BL/6 마우스를 이용한 육모효과에 있어서는 한방복합 처방단 처리 4주 후 98 % 이상의 육모효과를 나타내는 것을 확인하였다. 이상의 결과로 볼 때 본 연구에서 개발한 한방복합처방단은 모발의 성장 촉진에 유용하게 활용될 수 있는 처방으로 사료된다.

Abstract ▼ AI-Helper

To develop new therapeutic materials to prevent hair loss and enhance hair growth, we developed a medicinal herbal complex extract (MHCE) using 23 herbs traditionally used in oriental medicine. Medicinal Herbal complex extract was consist of Angelica gigas Nakai, Psoralea corylifolia Linne, Biota orientalis Endlicher, and Eclipta prostrata Linne, Rehmannia glutinosa Liboschitz var. purpurea Makino, Ligustrum lucidum Aiton, Polygonum multiflorum Thunberg, and Sesamum indicum Linne, Sophora angustifolia Sieboldet Zuccarini, Angelica dahurica Benthamet Hooker, and Leonurus sibiricus Linne, Salvia miltiorrhiza Bunge, Prunus persica Batsch, Commiphora molmol Engler, Chrysanthemum indicum Linne, Boswellia carterii Birdwood, Panax ginseng C. A. Meyer, Cnidium officinale Makino, Albizia julibrissin Durazzini, and Corydalis ternata Nakai that have traditionally been used for treating hair loss, preventing gray hair, anti-inflammation, and blood circulation in oriental medicine. In addition, we examined the hair growth effect of MHCE in vitro and in vivo. In vitro, we evaluated the effects of MHCE on cultured HFDPC, HaCaT cells, and murine embryonal fibroblasts (NIH3T3 cells). Also, we evaluated the ability of MHCE to prevent gray hair on murine melanoma cells (B16F1 cells). The hair growth-promoting effect of MHCE in vitro was also observed in vivo using C57BL/6 mice. Our results showed that MHCE significantly increased the proliferation of HFDPC (175 % proliferation at $50{\mu}g/mL$), HaCaT cells (133 % proliferation at $20{\mu}g/mL$), and NIH3T3 cells (120 % proliferation at $50{\mu}g/mL$). MHCE also showed consistent melanogenesis in B16F1 cells (154 % melanin synthesis at $50{\mu}g/mL$). Moreover, MHCE showed potential for hair growth stimulation in C57BL/6 mice experiments (98 % hair growth area on 4 weeks). These results indicate that MHCE may be a good candidate for promotion of hair growth.

주제어

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가설 설정

To investigate the effects of MHCE on cell growth in hair follicles, we examined whether MHCE influenced the proliferation of HFDPC and HaCaT cells.

제안 방법

For quantitative comparison of hair growth, the image files for the photographs were used to calculate hair growth areas. Hair growth area was expressed as the percent area of hair growth of the total depilated area of the back using Image Analysis Software(Image Partner; Saramsoft, Korea).
1 % and 1 % MHCE in 75 % Ethanol, respectively. From the following day (day 1), 0.2 mL of the minoxidil, 0.1 % MHCE, 1 % MHCE and vehicle was topically applied to the back twice daily for 28 days. Hair growth promotion was evaluated by observing darkening of the skin color, indicating telogen-to-anagen conversion, and photographed with a digital camera at 0, 1, 2, 3, and 4 weeks after depilation.
Figure 4. Hair growth area (%) in an alopecia model of C57BL/6 male mice treated with 75 % Ethanol, 3 % minoxidil, 0.1 % MHCE, and 1 % MHCE at 2 and 4 weeks. *p < 0.
1 % MHCE, 1 % MHCE and vehicle was topically applied to the back twice daily for 28 days. Hair growth promotion was evaluated by observing darkening of the skin color, indicating telogen-to-anagen conversion, and photographed with a digital camera at 0, 1, 2, 3, and 4 weeks after depilation.
This strict coupling leads to characteristic changes in skin pigmentation during the anagen phase development [25-27]. In this study, we examined the hair growth effect of MHCE in vitro and in vivo. First, we evaluated the effects of MHCE on cultured HFDPC, HaCaT cells, and murine embryonal fibroblasts (NIH3T3 cells).
Figure 5. Representative histological observations of hair growth in an alopecia model of C57BL/6 male mice treated with 75 % Ethanol, 3 % minoxidil, 0.1 % MHCE, and 1 % MHCE at 2 and 4 weeks.

대상 데이터

The anagen phase was then induced in the back skin of the six-week-old male C57BL/6 mice by shaving, which led to synchronized development of the anagen hair follicles. Forty-eight animals in four randomized groups (n = 12) were used for the study. The animals in group 1 received an equal volume of the vehicle (75 % Ethanol) as a control group.
HFDPC, HaCaT cells, NIH3T3 cells, and B16F1 cells were obtained from PromoCell, Cosmax Inc. (Korea), the Korean Collection for Type Cultures (Korea), and the American Type Culture Collection (USA), respectively. HFDPC was maintained in follicle dermal papilla cell growth medium.
Male C57BL/6 mice (5 weeks of age) were purchased from Koatech (Korea) and were acclimated for one week in a breeding room. Temperature and humidity were maintained at 22 ± 2 ℃ and 50 ± 20 % respectively, and the light cycle was automatically maintained (12 h light/darkness).

데이터처리

The data were analyzed using a Student’s t-test using SPSS (SPSS Inc., USA).

성능/효과

In conclusion, the results of this study demonstrate that MHCE promotes the hair growth in vitro via proliferation of dermal papilla cells, keratinocytes, and NIH3T3 cells. In addition, MHCE has a gray hair preventative effect via increase of melaninsynthesis in B16F1 melanoma cells.
Lycium chinense Miller, Rubus coreanus Miquel, Rehmannia glutinosa Liboschitz var. purpurea Makino, Sesamum indicum Linne, Angelica dahurica Benthamet Hooker, and Leonurus sibiricus Linne, Prunus persica Batsch, Panax ginseng C. A. Meyer, Cnidium officinale Makino, and Albizia julibrissin Durazzini were domestic and Morus alba Linne, Ligustrum lucidum Aiton, Polygonum multiflorum Thunberg, Sophora angustifolia Sieboldet Zuccarini, Salvia miltiorrhiza Bunge, Commiphora molmol Engler, Chrysanthemum indicum Linne, Boswellia carterii Birdwood, and Corydalis ternata Nakai were imported from China.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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