Development of a Practical and Cost-Effective Medium for Bioethanol Production from the Seaweed Hydrolysate in Surface-Aerated Fermentor by Repeated-Batch Operation원문보기
To develop a practical and cost-effective medium for bioethanol production from the hydrolysate of seaweed Sargassum sagamianum, we investigated the feasibility and performance of bioethanol production in CSL (corn-steep liquor)-containing medium, where yeast Pichia stipitis was used and the repeate...
To develop a practical and cost-effective medium for bioethanol production from the hydrolysate of seaweed Sargassum sagamianum, we investigated the feasibility and performance of bioethanol production in CSL (corn-steep liquor)-containing medium, where yeast Pichia stipitis was used and the repeated batch was carried out in a surface-aerated fermentor. The optimal medium replacement time during the repeated operation was determined to be 36 h, and the surface aeration rates were 30 and 100 ml/min. Under these conditions, the repeated-batch operation was successfully carried out for 6 runs (216 h), in which the maximum bioethanol concentrations reached about 11-12 g/l at each batch operation. These results demonstrated that bioethanol production could be carried out repeatedly and steadily for 216 h. In these experiments, the total cumulative bioethanol production was 57.9 g and 58.0 g when the surface aeration rates were 30 ml/min and 100 ml/min, respectively. In addition, the bioethanol yields were 0.43 (about 84% of theoretical value) and 0.44 (about 86% of theoretical value) when the surface aeration rates were 30 ml/min and 100 ml/min, respectively. CSL was successfully used as a medium ingredient for the bioethanol production from the hydrolysate of seaweed Sargassum sagamianum, indicating that this medium may be practical and cost-effective for bioethanol production.
To develop a practical and cost-effective medium for bioethanol production from the hydrolysate of seaweed Sargassum sagamianum, we investigated the feasibility and performance of bioethanol production in CSL (corn-steep liquor)-containing medium, where yeast Pichia stipitis was used and the repeated batch was carried out in a surface-aerated fermentor. The optimal medium replacement time during the repeated operation was determined to be 36 h, and the surface aeration rates were 30 and 100 ml/min. Under these conditions, the repeated-batch operation was successfully carried out for 6 runs (216 h), in which the maximum bioethanol concentrations reached about 11-12 g/l at each batch operation. These results demonstrated that bioethanol production could be carried out repeatedly and steadily for 216 h. In these experiments, the total cumulative bioethanol production was 57.9 g and 58.0 g when the surface aeration rates were 30 ml/min and 100 ml/min, respectively. In addition, the bioethanol yields were 0.43 (about 84% of theoretical value) and 0.44 (about 86% of theoretical value) when the surface aeration rates were 30 ml/min and 100 ml/min, respectively. CSL was successfully used as a medium ingredient for the bioethanol production from the hydrolysate of seaweed Sargassum sagamianum, indicating that this medium may be practical and cost-effective for bioethanol production.
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문제 정의
stipitis was conducted as previously described [30, 31] and we investigated whether both the hydrolysate of seaweed and CSL could be used for bioethanol production. Thus, the aim of this study was to examine the feasibility and the performance of bioethanol production from medium containing the hydrolysate of seaweed S. sagamianum and CSL. The use of CSL as a medium ingredient is expected to make bioethanol production more practical and cost-effective.
가설 설정
In this study, repeated-batch operation in the surface-aerated fermentor using P. stipitis was conducted as previously described [30, 31] and we investigated whether both the hydrolysate of seaweed and CSL could be used for bioethanol production. Thus, the aim of this study was to examine the feasibility and the performance of bioethanol production from medium containing the hydrolysate of seaweed S.
sagamianum. Therefore, in this study, we investigated the potential of using corn-steep liquor (CSL) as a substitute for yeast extract and peptone. CSL is a by-product of corn wet-milling, and an important constituent in microbial growth media as an excellent source of organic nitrogen.
제안 방법
4B and 4D). The bioethanol yields were calculated based on sugar levels using the DNS assay and quantitative TLC analysis. The CSC, which was measured using the DNS assay, and the TLC analysis produced very similar values.
대상 데이터
Dinitrosalicylic acid and α-naphtol were obtained from Samchun Chemical Co. (Seoul, Republic of Korea) and Sigma-Aldrich (St. Louis, MO, USA), respectively.
이론/모형
5% α-naphtol and 5% H2SO4 in ethanol and then dried in an oven at 80℃ for 15 min. The amount of glucose and xylose in the culture broth were quantitatively determined via TLC using the method described by Robyt and Mukerjea [23]. Using this approach, the intensity and density of the glucose, isomaltodextrin, and maltodextrin on the TLC plate was the same based on weight, and the density was linearly proportional to the weight of the sugar compound.
성능/효과
4B and 4D, this was not observed, which may have occurred if the DNS assay underestimated the CSC. The bioethanol yields, which were calculated from the slopes of Fig. 4B and 4D and were averaged with the values determined from both the DNS assay and quantitative TLC analysis, were 0.43 (about 84% of theoretical value) and 0.44 (about 86% of theoretical value) when the surface aeration rates were 30 ml/min and 100 ml/min, respectively (Fig. 4B and 4D). These two values were very close to those reported in a previous study [31], in which the bioethanol yield was determined to be approximately 0.
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