[논문]청국장에서 분리한 Bacillus subtilis GS-2에 의한 Poly(γ-Glutamic Acid) 생산의 최적 배양조건

방병호; 이문수; 김관필; 이동희

doi:10.9799/ksfan.2012.25.3.677

청국장에서 분리한 Bacillus subtilis GS-2에 의한 Poly(γ-Glutamic Acid) 생산의 최적 배양조건
Influences of Culture Medium Components on the Production Poly (γ-Glutamic Acid) by Bacillus subtilis GS-2 Isolated Chungkookjang 원문보기

한국식품영양학회지 = The Korean journal of food and nutrition, v.25 no.3, 2012년, pp.677 - 684

방병호 (을지대학교 식품영양학과) , 이문수 (한국생명공학연구원) , 김관필 (롯데제과) , 이동희 (건국대학교 미생물공학과)

초록
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Poly-${\gamma}$-glutamic Acid(${\gamma}$-PGA)를 다량 생산하는 균주를 우리나라의 전통발효식품인 청국장으로부터 Bacillus subtilis GS-2를 분리하였다. 이 균은 glutamic acid 의존형 균으로, 이 균에 의한 ${\gamma}$-PGA 생산 최적 조건을 검토한 바, 단순배지(L-glutamic acid 2.0%, glucose 1.0%, $NH_4Cl$ 0.5%, $KH_2PO_4$ 0.05%, $MgSO_4$ $7H_2O$ 0.01%, pH 7.0)로 진탕배양(220 rpm) 하였을 때, 배양시간 48시간, 최적온도 $33^{\circ}C$, 그리고 초기 pH 6.5로 나타났다. 영양원으로 glutamic acid 3%, sucrose 3%, $NH_4Cl$ 0.25%, $KH_2PO_4$ 0.15%, $MgSO_4$ $7H_2O$ 0.015%에서 ${\gamma}$-PGA 최대 생산량이 31.0 $g/{\ell}$이었다.

Abstract ▼ AI-Helper

A bacterium strain GS-2 isolated from the Korean traditional seasoning food, Chungkookjang and was determined to produce large amounts of ${\gamma}$-PGA with high productivity when provided with simple nutrients (L-glutamic acid 2.0%, glucose 1.0%, $NH_4Cl$ 0.5%, $KH_2PO_4$ 0.05%, $MgSO_4{\cdot}7H_2O$ 0.01%, pH 7.0). In this study, the culture medium for this strain was optimized for the production of ${\gamma}$-PGA. The Bacillus subtilis GS-2 required supplementation with L-glutamic acid and other nutrients for maximal production of ${\gamma}$-PGA. The optimal culture conditions for ${\gamma}$-PGA production were a 48 hr culture time, a temperature of $33^{\circ}C$ and initial pH of 6.5 by rotary shaking (220 rpm). A maximum ${\gamma}$-PGA production of 31.0 $g/{\ell}$ was obtained with L-glutamic acid (30 $g/{\ell}$), sucrose (the main carbon source, 30 $g/{\ell}$), $NH_4Cl$ (the main nitrogen source, 2.5 $g/{\ell}$), $KH_2PO_4$ (1.5 $g/{\ell}$) and $MgSO_4{\cdot}7H_2O$ (0.15 $g/{\ell}$) in the culture medium.

주제어

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제안 방법

All experiments were carried out in triplicate and the data were expressed as mean±SD of triple determinations.

이론/모형

The residual protein content in the purified γ-PGA pre-paration was determined by the Bradford dye binding method (1976).

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