• 검색어에 아래의 연산자를 사용하시면 더 정확한 검색결과를 얻을 수 있습니다.
  • 검색연산자
검색연산자 기능 검색시 예
() 우선순위가 가장 높은 연산자 예1) (나노 (기계 | machine))
공백 두 개의 검색어(식)을 모두 포함하고 있는 문서 검색 예1) (나노 기계)
예2) 나노 장영실
| 두 개의 검색어(식) 중 하나 이상 포함하고 있는 문서 검색 예1) (줄기세포 | 면역)
예2) 줄기세포 | 장영실
! NOT 이후에 있는 검색어가 포함된 문서는 제외 예1) (황금 !백금)
예2) !image
* 검색어의 *란에 0개 이상의 임의의 문자가 포함된 문서 검색 예) semi*
"" 따옴표 내의 구문과 완전히 일치하는 문서만 검색 예) "Transform and Quantization"
쳇봇 이모티콘
ScienceON 챗봇입니다.
궁금한 것은 저에게 물어봐주세요.

논문 상세정보

비파 잎의 추출용매별 항산화성 검증과 활성물질의 분리 및 동정

Isolation and Identification of Antioxidant Compounds of Various Solvents Extracted from Eriobotrya japonica Leaves


비파 잎의 항산화 활성을 탐색하고 활성물질을 분리하여 규명하기 위하여, 동결 건조한 비파 잎을 마쇄하여 n-hexane, ethyl acetate (EtOAc)와 methanol (MeOH)로 용매 분획하였다. 각 추출물에 대하여 정량적으로 DPPH 소거활성, SOD 유사활성을 측정하고, 페놀 화합물과 플라보노이드 함량을 측정하였으며, 각 용매에 따른 추출물 중 비교적 활성이 강한 EtOAc 추출물과 MeOH 추출물에 대하여 column chromatography를 이용하여 얻은 분획물들 중 강한 활성을 보인 각 1개의 분획물에서 3개의 화합물을 분리하였으며, GC/MS 분석에서 phytol, ${\beta}$-sitosterol 및 (-)-Loliolide으로 동정하였다.


To investigate potential medicinal or functional uses of Eriobotrya japonica, this study focused on the isolation and identification of antioxidant compounds from Eriobotrya japonica leaves. Various solvents were extracted from the leaves, and their scavenging effect on 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals was measured, in addition to their superoxide dismutase-like activity, polyphenol compounds, and flavonoid content. Ethyl acetate extract exhibited the strongest scavenging effect in a 0.2 mM solution of DPPH ($63.24{\pm}2.20%$, $81.83{\pm}2.10%$, and $93.15{\pm}2.31%$ in 0.3, 0.7, and 1.0 mg/ml sample concentrations, respectively). The antioxidant effect of the ethyl acetate extract and methanol extract were generally stronger than that of n-hexane extract. The extracts were further purified by repeated silica gel column chromatography. The antioxidant compounds were identified as phytol, ${\beta}$-sitosterol, and (-)-loliolide using GC/MS.

참고문헌 (43)

  1. Bae, Y. I. and Shim, K. H. 1998. Nutrition components in different parts of Korean loquat (Eriobotrya japonica Lindl.). Kor. J. Postharvest Sci. Technol. 5, 57-63. 
  2. Bae, Y. I., Chung, Y. C. and Shim, K. H. 2002. Antimicrobial and antioxidant activities of various solvent extract from different parts of loquat (Eriobotrya japonica, Lindl.). Kor. J. Food Preserv. 9, 97-101. 
  3. Bae, Y. I., Jeong, C. H. and Shim, K. H. 2005. Antioxidative and antimicrobial activity of epicatechin isolated from leaves of loquat (Eriobotrya japonica). J. Food Sci. Nutr. 10, 118-121. 
  4. Bae, Y. I., Jeong, C. H. and Shim, K. H. 2002. Nitrite-scavenging and antimutagenic effects of various solvent extract from different parts of loquat (Eriobotrya japonica, Lindl.). Kor. J. Food Preserv. 9, 92-96. 
  5. Bae, Y. I., Moon, J. S. and Shim, K. H. 1998. Loquat (Eriobotrya japonica Lindl.) juice processing and its physicochemical properties. Kor. J. Postharvest Sci. Technol. 5, 270-274. 
  6. Bae, Y. I., Seo, K. I., Park, S. K. and Shim, K. H. 1998. Loquat (Eriobotrya japonica Lindl.) leaf tea processing and its physicochemical properties. Kor. J. Postharvest Sci. Technol. 5, 262-269. 
  7. Banno, N., Akihisa, T., Tokuda, H., Yasukawa, K., Taguchi, Y., Akazawa, H., Ukiya, M., Kimura, Y., Suzuki, T. and Nishino, H. 2005. Anti-inflammatory and antitumorpromoting effects of the triterpene acids from the leaves of Eriobotrya japonica. Biol. Pharm. Bull. 28, 1995-1999. 
  8. Bark, Y. S., Park, Y. J., Kim, H. J., Im, M. H., Lee, M. K., Kim, Y. M., Cho, J. Y. and Heo, B. G. 2008. Physioligical activity of ethanol extract from the different plant parts of loquat (Eriobotrya japonica Lindl.). Kor. J. Hort. Sci. Technol. 26, 75-80. 
  9. Bondet, V., Brand-Williams, W. and Berset, C. 1997. Kinetics and mechanisms of antioxidant activity using the DPPH free radical method. Food Sci. Technol.-Leb. 30, 609-615. 
  10. Brand-Williams, W., Cuvelier, M. E. and Berset, C. 1995. Use of a free radical method to evaluate antioxidant activity. Lebensm.-Wiss. u.-Technol. 28, 25-30. 
  11. Branen, A. L. 1975. Toxicology and biochemistry of butylated hydroxyanisole and butylated hydroxytoluene. J. Am. Oil Chem. Soc. 52, 59-63. 
  12. Calliste, C. A., Trouilla, P., Allais, D. P., Simon, A. and Duroux, J. L. 2001. Free radical scavenging activities measured by electron spin resonance spectroscopy and B16 cell antiproliferative behaviors of seven plants. J. Agric. Food Chem. 49, 3321-3327. 
  13. Chen, J., Li, W. L., Wu, J. L., Ren, B. R. and Zhang, H. Q. 2008. Hypoglycemic effects of a sesquiterpene glycoside isolated from leaves of loquat (Eriobotrya japonica (Thunb.) Lindl.). Phytomedicine 15, 98-102. 
  14. Cho, Y. S., Park, S. K. and Lee, H. Y. 1991. Composition of free sugars, organic acids and free amino acid in loquat flesh. Kor. J. Soc. Food Nutr. 20, 89-93. 
  15. Chung, H. S. 2001. Isolation of new bioactive phytochemicals from natural products. Food Ind. Nutr. 6, 53-59. 
  16. Eom, H. J., Kim, S. M., Pyo, B. S. and Lee, K. I. 2009. Changes of physiological activity by drying temperature in leaf of Eriobotrya japonica. Kor. J. Pharmacogn. 40, 178-183. 
  17. Folin, O. and Denis, W. 1912. On phosphotungstic-phosphomolybdic compounds as color reagents. J. Biol. Chem. 12, 239-243. 
  18. Go, J. K. and Park, S. I. 2005. Preparation of stirred yoghurt from milk added with Korean loquat (Eriobotrya japonica Lindley). Kor. J. Food Nutr. 18, 200-206. 
  19. Hamada, A., Yoshioka, S., Takuma, D., Yokota, J., Cui, T., Kusunose, M., Miyamura, M., Kuotani, S. and Nishioka, Y. 2004. The effect of Eriobotrya japonica seed extract on oxidative stress in adriamycin-induced nephropathy in rats. Biol. Pharm. Bull. 27, 1961-1964. 
  20. Huang, Y., Li, J., Meng, X. M., Jiang, G. L., Li, H., Cao, Q., Yu, S. C., Lv, X. W. and Cheng, W. M. 2009. Effect of triterpene acids of Eriobotrya japonica (Thunb.) Lindl. leaf and MAPK signal transduction pathway on inducible nitric oxide synthase expression in alveolar macrophage of chronic bronchitis rats. Am. J. Chin. Med. 37, 1099-1111. 
  21. Jeong, Y. S., Jung, H. K., Youn, K. S., Kim, M. O. and Hong, J. H. 2009. Physiological activity of the hot water extract from Eriobotrya japonica Lindl. Kor. Soc. Food Sci. Nutr. 38, 977-982. 
  22. Jung, H. A., Park, J. C., Chung, H. Y., Kim, J. and Choi, J. S. 1999. Antioxidant flavonoids and chlorogenic acid from the leaves of Eriobotrya japonica. Arch. Pharm. Res. 22, 213-218. 
  23. Kim, E., Kim, M. S., Rhyu, D. Y., Min, O. J., Baek, H. Y., Kim, Y. J. and Kim, H. A. 2009. Hypoglycemic effect of Eriobotrya japonica (E. japonica) in db/db mice. Kor. J. Food Nutr. 22, 159-165. 
  24. Kim, H. J., Jo, C. H., Kim, T. H., Kim, D. S., Park, M. Y. and Byun, M. W. 2006. Biological evaluation of the methanolic extract of Eriobotrya japonica and its irradiation effect. Kor. J. Food. Sci. Technol. 38, 684-690. 
  25. Kim, M. S., You, M. K., Rhyu, D. Y., Kim, Y. J., Baek, H. Y. and Kim, H. A. 2009. Loquat (Eriobotrya japonica) extracts suppress the adhesion, migration and invasion of human breast cancer cell line. Nutr. Res. Practice 3, 259-264. 
  26. Kim, S. H. and Shin, T. Y. 2009. Anti-inflammatory effect of leaves of Eriobotrya japonica correlating with attenuation of p38 MAPK, ERK, and NF-${\kappa}B$ activation in mast cells. J. Toxicol. in Vitro 23, 1215-1219. 
  27. Kim, T. H., Shin, S. R., Kim, T. W., Lee, I. C., Park, M. Y. and Jo, C. 2009. A tyrosinase inhibitor isolated from the seeds of Eriobotrya japonica. Kor. J. Food Preserv. 16, 435-441. 
  28. Koba, K., Matsuoka, A., Osada, K. and Huang, Y. S. 2007. Effect of loquat (Eriobotrya japonica) extracts on LDL oxidation. J. Food Chem. 104, 308-316. 
  29. Lee, B. Y., Park, E. M., Kim, E. J., Choi, H. D., Kim, I. H. and Hwang, J. B. 1996. Analysis of chemical components of Korean loquat (Eriobotrya japonica Lindl.) fruit. Kor. J. Food Sci. Technol. 28, 428-432. 
  30. Lee, C. B. 1982. Korean pictorial book of plants. pp. 684-687. Hyangmoonsa. Seoul. 
  31. Lee, K. I. and Kim, S. M. 2009. Antioxidative and antimicrobial activities of Eriobotrya japonica Lindl. leaf extracts. Kor. J. Soc. Food Sci. Nutr. 38, 267-273. 
  32. Lee, Y. S. 2008. Analysis of components in the different part of Ailanthus altissima. Kor. J. Food Preserv. 15, 261-268. 
  33. Marklund, S. and Marklund, G. 1974. Involvement of superoxide onion radical in the oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biol. Chem. 47, 468-474. 
  34. Min, O. J., Oh, J., Kim, H. A., Kim, M. S., Baek, H. Y., Kim, Y. J. and Rhyu, D. Y. 2010. Effect of Eriobotrya japonica leaf and seed extracts on adipogenesis. Kor. J. Pharmacogn. 41, 270-274. 
  35. Nozato, N., Matsumoto, K. and Uemitsu, N. 1994. Triterpenes from leaves of Eriobotrya japonica. J. Nat. Med. 48, 336. 
  36. Ruomei, G., Zhuobin, Y., Zhiqiang, Z. and Xiurui, G. 1998. Mechanism of pyrogallol autoxidation and determination of superoxide dismutase enzyme activity. J. Bioelectrochem. Bioenergetics 45, 41-45. 
  37. Shimizu, M., Uemitsu, N., Shirota, M., Matsumato, K. and Tezuka, Y. 1996. A new triterpene ester from Eriobotrya japonica. J. Chem. Pharm. Bull. 44, 2191-2182. 
  38. Sun, G., Zhang, Y., Takuma, D., Onogawa, M., Yokota, J., Hamada, A., Yoshioka, S., Kusunose, M., Miyamura, M., Kyotani, S. and Nishioka, Y. 2007. Effect of orally administered Eriobotrya japonica seed extract on allergic contact dermatitis in rats. J. Pharm. Pharmacol. 59, 1405-1412. 
  39. Takuma, D., Guangchen, S., Yokota, J., Hamada, A., Onogawa, M., Yoshioka, S., Kusunose, M., Miyamura, M., Kyotani, S. and Nishioka, Y. 2008. Effect of Eriobotrya japonica seed extract on 5-fluorouracil-induced mucositis in hamsters. J. Biol. Pharm. Bull. 31, 250-254. 
  40. Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T. D., Mazur, M. and Telser, J. 2007. Free radical and antioxidants in normal physiological function and human disease. J. Biochem. Cell Biol. 39, 44-84. 
  41. Whang, T. E., Lim, H. O. and Lee, J. W. 1996. Anticancer effect of Eriobotrya japonica Lindl by specificity test with several cancer cell lines. Kor. J. Medicinal Crop Sci. 4, 314-320. 
  42. Yokota, J., Takuma, D., Hamada, A., Onogawa, M., Yoshioka, S., Kusunose, M., Miyamura, M., Kyotani, S. and Nishioka, Y. 2006. Scavenging of reactive oxygen species by Eriobotrya japonica seed extract. J. Biol. Pharm. Bull. 29, 467-471. 
  43. Zong, W. and Zhao, G. 2007. Corosolic acid isolation from the leaves of Eriobotrta japonica showing the effects on carbohydrate metabolism and differentiation of 3T3-L1 adipocytes. Asia Pac. J. Clin. Nutr. 16, 346-352. 

이 논문을 인용한 문헌 (1)

  1. 2014. "" 원예과학기술지 = Korean journal of horticultural science & technology, 32(4): 558~570 


원문 PDF 다운로드

  • ScienceON :
  • KCI :

원문 URL 링크

원문 PDF 파일 및 링크정보가 존재하지 않을 경우 KISTI DDS 시스템에서 제공하는 원문복사서비스를 사용할 수 있습니다. (원문복사서비스 안내 바로 가기)

상세조회 0건 원문조회 0건

이 논문과 연관된 기능

DOI 인용 스타일