$\require{mediawiki-texvc}$

연합인증

연합인증 가입 기관의 연구자들은 소속기관의 인증정보(ID와 암호)를 이용해 다른 대학, 연구기관, 서비스 공급자의 다양한 온라인 자원과 연구 데이터를 이용할 수 있습니다.

이는 여행자가 자국에서 발행 받은 여권으로 세계 각국을 자유롭게 여행할 수 있는 것과 같습니다.

연합인증으로 이용이 가능한 서비스는 NTIS, DataON, Edison, Kafe, Webinar 등이 있습니다.

한번의 인증절차만으로 연합인증 가입 서비스에 추가 로그인 없이 이용이 가능합니다.

다만, 연합인증을 위해서는 최초 1회만 인증 절차가 필요합니다. (회원이 아닐 경우 회원 가입이 필요합니다.)

연합인증 절차는 다음과 같습니다.

최초이용시에는
ScienceON에 로그인 → 연합인증 서비스 접속 → 로그인 (본인 확인 또는 회원가입) → 서비스 이용

그 이후에는
ScienceON 로그인 → 연합인증 서비스 접속 → 서비스 이용

연합인증을 활용하시면 KISTI가 제공하는 다양한 서비스를 편리하게 이용하실 수 있습니다.

생체외 모델에서 아우레오바지디움 유래 베타 글루칸이 피부창상 치유에 미치는 영향

Effect of ${\\beta}$-glucan Originated from Aureobasidium on the Dermal Wound Repair in Vitro Model

Journal of veterinary clinics = 한국임상수의학회지, v.30 no.6, 2013년, pp.403 - 408  

이정우 (경북대학교 수의과대학) ,  권영삼 (경북대학교 수의과대학) ,  장광호 (경북대학교 수의과대학)

초록
AI-Helper 아이콘AI-Helper

본 연구의 목적은 in vitro 실험에서 human dermal fibroblast cell을 이용한 섬유아세포 증식과 콜라겐 생성을 통한 전층피부창상치유에 대한 효과를 알아보는 것이다. In vitro 실험에서, 베타글루칸 및 양성대조물질 oncostatin M을 사용하여 처치 후 24 또는 48시간 이후 MTT assay와 세포수 관찰을 통해 섬유모세포 증식효과에 대해 평가하였다. Procollagen 생산에 대한 효과를 평가하기 위해 베타글루칸 및 양성대조물질 oncostatin M 처치 48시간 후 hydroxyproline을 HPLC를 이용하여 정량하여 평가하였다. 창상재구축에 대한 효과를 평가하기 위해 베타글루칸 및 양성대조물질 fucoidan fraction 7을 FPCL 모델을 이용하여 평가하였다. 베타글루칸 처치군은 음성대조군 대비 0.1 mg/ml 이상의 농도에서 용량의존적이고 유의적인 OD 또는 섬유아세포수의 증가가 관찰되었다. 게다가 베타글루칸 처치군은 창상면으로 유주된 세포수가 음성대조군 대비 1 mg/ml 이상의 농도에서 용량의존적이고 유의적인 증가가 관찰되었다. 그러나 베타글루칸 처치군은 음성대조군 대비 모든 용량에서 procollagen 생산에 대한 유의적인 변화가 관찰되지 않았다.

Abstract AI-Helper 아이콘AI-Helper

The objective of the present study is to detect the effect of ${\beta}$-glucan originated from Aureobasidium on the proliferation and collagen production in human dermal fibroblast cells with wound repopulation in vitro. The proliferative effects were assessed using a MTT assay as well as...

주제어

AI 본문요약
AI-Helper 아이콘 AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

  • Ten microliters of un-polymerised collagen was used to adhere the FPCL to the underlying collagen lattice. Defect repopulation was then measured by counting the number of cells which had migrated from the cut edge of the lattice at 3 days post wounding and treatment of test articles. Results are expressed as a mean of cell counts in ten identical photographic fields from ten replicated wounds
  • This study, therefore, was carried out to examine the effect of β-glucan originated from Aureobasidium, on the proliferation and collagen production human dermal fibroblast cells with wound repopulation in in vitro models.

대상 데이터

  • The normal diploid human fetal dermal Fibroblast cell line (FW20-2) was obtained from National Institute of General Medical Sciences (NIGMS, NJ, USA) and was used between passages 12 and 18. Primary human dermal fibroblasts were established from explant cultures (28).

데이터처리

  • (n = 10). Statistical analyses were conducted using Mann-Whitney U-Wilcoxon Rank Sum W test (MW test) with SPSS for Windows (Release 6.1.3., SPSS Inc., USA). The inhibition rate compared to that of vehicle control was calculated to help the understanding of the efficacy of test materials on differences between vehicle control and test groups
본문요약 정보가 도움이 되었나요?

참고문헌 (46)

  1. Bamber B, Reife RA, Haugen HS, Clegg CH. Oncostatin M stimulates excessive extracellular matrix accumulation in a transgenic mouse model of connective tissue disease. J Mol Med 1998; 76: 61-69. 

  2. Bayat A, McGrouther DA, Ferguson MW. Skin scarring. BMJ 2003; 326: 88-92. 

  3. Bell S, Goldman VM, Bistrian BR, Arnold AH, Ostroff G, Forse RA. Effect of beta-glucan from oats and yeast on serum lipids. Crit Rev Food Sci Nutr 1999; 39: 189-202. 

  4. Berteau O, Mulloy B. Sulfated fucans, fresh perspectives: structures, functions, and biological properties of sulfated fucans and an overview of enzymes active toward this class of polysaccharide. Glycobiology 2003; 13: 29R-40R 

  5. Border WA, Noble NA. Transforming growth factor beta in tissue fibrosis. N Engl J Med 1994; 331: 1286-1292. 

  6. Brewster CE, Howarth PH, Djukanovic R, Wilson J, Holgate ST, Roche WR. Myofibroblasts and subepithelial fibrosis in bronchial asthma. Am J Respir Cell Mol Biol 1990; 3: 507-511. 

  7. Campa JS, McAnulty RJ, Laurent GJ. Application of highpressure liquid chromatography to studies of collagen production by isolated cells in culture. Anal Biochem 1990; 186: 257-263. 

  8. Cattaruzza S, Perris R. Proteoglycan control of cell movement during wound healing and cancer spreading. Matrix Biol 2005; 24: 400-417. 

  9. Chambers RC, Dabbagh K, McAnulty RJ, Gray AJ, Blanc- Brude OP, Laurent GJ. Thrombin stimulates fibroblast procollagen production via proteolytic activation of proteaseactivated receptor 1. Biochem J 1998; 333: 121-127. 

  10. Chung KF, Barnes PJ. Cytokines in asthma. Thorax 1999; 54: 825-857. 

  11. Clark RA. Biology of dermal wound repair. Dermatol Clin 1993; 11: 647-666. 

  12. Czaja W, Krystynowicz A, Bielecki S, Brown RM Jr. Microbial cellulose--the natural power to heal wounds. Biomaterials 2006; 27: 145-151. 

  13. Czop JK. The role of beta-glucan receptors on blood and tissue leukocytes in phagocytosis and metabolic activation. Pathol Immunopathol Res 1986; 5: 286-296. 

  14. Delatte SJ, Evans J, Hebra A, Adamson W, Othersen HB, Tagge EP. Effectiveness of beta-glucan collagen for treatment of partial-thickness burns in children. J Pediatr Surg 2001; 36: 113-118. 

  15. Deroanne CF, Colige AC, Nusgens BV, Lapiere CM. Modulation of expression and assembly of vinculin during in vitro fibrillar collagen-induced angiogenesis and its reversal. Exp Cell Res 1996; 224: 215-223. 

  16. Deters AM, Lengsfeld C, Hensel A. Oligo- and polysaccharides exhibit a structure-dependent bioactivity on human keratinocytes in vitro. J Ethnopharmacol 2005; 102: 391-399. 

  17. Duncan MR, Hasan A, Berman B. Oncostatin M stimulates collagen and glycosaminoglycan production by cultured normal dermal fibroblasts: insensitivity of sclerodermal and keloidal fibroblasts. J Invest Dermatol 1995; 104: 128-133. 

  18. Estrada A, Yun CH, Van Kessel A, Li B, Hauta S, Laarveld B. Immunomodulatory activities of oat beta-glucan in vitro and in vivo. Microbiol Immunol 1997; 41: 991-998. 

  19. Froget S, Barthelemy E, Guillot F, Soler C, Coudert MC, Benbunan M, Dosquet C. Wound healing mediator production by human dermal fibroblasts grown within a collagen- GAG matrix for skin repair in humans. Eur Cytokine Netw 2003; 14: 60-64. 

  20. Fujimura T, Tsukahara K, Moriwaki S, Kitahara T, Sano T, Takema Y. Treatment of human skin with an extract of Fucus vesiculosus changes its thickness and mechanical properties. J Cosmet Sci 2002; 53: 1-9. 

  21. Gan L, Fagerholm P, Kim HJ. Effect of leukocytes on corneal cellular proliferation and wound healing. Invest Ophthalmol Vis Sci 1999; 40: 575-581. 

  22. Genever PG, Wood EJ, Cunliffe WJ. The wounded dermal equivalent offers a simplified model for studying wound repair in vitro. Exp Dermatol 1993; 2: 266-273. 

  23. Grinnell F. Fibroblasts, myofibroblasts, and wound contraction. J Cell Biol 1994; 124: 401-404. 

  24. Harding KG, Morris HL, Patel GK. Science, medicine and the future: healing chronic wounds. BMJ 2002; 324: 160-163. 

  25. Ihn H, LeRoy EC, Trojanowska M. Oncostatin M stimulates transcription of the human alpha2(I) collagen gene via the Sp1/Sp3-binding site. J Biol Chem 1997; 272: 24666-24672. 

  26. Jeffery PK. Morphology of the airway wall in asthma and in chronic obstructive pulmonary disease. Am Rev Respir Dis 1991; 143: 1152-1158. 

  27. Jia Y, Turek JJ. Inducible nitric oxide synthase links NFkappaB to PGE2 in polyunsaturated fatty acid altered fibroblast in-vitro wound healing. Lipids Health Dis 2005; 4: 14-23. 

  28. Keerthisingam CB, Jenkins RG, Harrison NK, Hernandez- Rodriguez NA, Booth H, Laurent GJ, Hart SL, Foster ML, McAnulty RJ. Cyclooxygenase-2 deficiency results in a loss of the anti-proliferative response to transforming growth factor-beta in human fibrotic lung fibroblasts and promotes bleomycin-induced pulmonary fibrosis in mice. Am J Pathol 2001; 158: 1411-1422. 

  29. Lee SB, Jeon HW, Lee YW, Lee YM, Song KW, Park MH, Nam YS, Ahn HC. Bio-artificial skin composed of gelatin and (1-->3), (1-->6)-beta-glucan. Biomaterials 2003; 24: 2503-2511. 

  30. Lia A, Hallmans G, Sandberg AS, Sundberg B, Aman P, Andersson H. Oat beta-glucan increases bile acid excretion and a fiber-rich barley fraction increases cholesterol excretion in ileostomy subjects. Am J Clin Nutr 1995; 62: 1245-1251. 

  31. Lin YC, Grinnell F. Decreased level of PDGF-stimulated receptor autophosphorylation by fibroblasts in mechanically relaxed collagen matrices. J Cell Biol 1993; 122: 663-672. 

  32. Mekkes JR, Loots MA, Van Der Wal AC, Bos JD. Causes, investigation and treatment of leg ulceration. Br J Dermatol 2003; 148: 388-401. 

  33. Mochitate K, Pawelek P, Grinnell F. Stress relaxation of contracted collagen gels: disruption of actin filament bundles, release of cell surface fibronectin, and down-regulation of DNA and protein synthesis. Exp Cell Res 1991; 193: 198-207 

  34. O'Leary R, Arrowsmith M, Wood EJ. Characterization of the living skin equivalent as a model of cutaneous reepithelialization. Cell Biochem Funct 2002; 20: 129-141. 

  35. O'Leary R, Rerek M, Wood EJ. Fucoidan modulates the effect of transforming growth factor (TGF)-beta1 on fibroblast proliferation and wound repopulation in in vitro models of dermal wound repair. Biol Pharm Bull 2004; 27: 266-270. 

  36. Peplow PV. Glycosaminoglycan: a candidate to stimulate the repair of chronic wounds. Thromb Haemost 2005; 94: 4-16. 

  37. Rose TM, Bruce AG. Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. Proc Natl Acad Sci U S A 1991; 88: 8641-8645. 

  38. Rowling PJ, Raxworthy MJ, Wood EJ, Kearney JN, Cunliffe WJ. Fabrication and reorganization of dermal equivalents suitable for skin grafting after major cutaneous injury. Biomaterials 1990; 11: 181-185. 

  39. Scaffidi AK, Mutsaers SE, Moodley YP, McAnulty RJ, Laurent GJ, Thompson PJ, Knight DA. Oncostatin M stimulates proliferation, induces collagen production and inhibits apoptosis of human lung fibroblasts. Br J Pharmacol 2002; 136: 793-801. 

  40. Seo HP, Kim JM, Shin HD, Kim TK, Chang HJ, Park BR, Lee JW. Production of -1,3/1,6-glucan by Aureobasidium pullulans SM-2001. Korean J Bitechnol Bioeng 2002; 17: 376-380. 

  41. Singer AJ, Clark RA. Cutaneous wound healing. N Engl J Med 1999; 341: 738-746 

  42. Stephens P, Genever PG, Wood EJ, Raxworthy MJ. Integrin receptor involvement in actin cable formation in an in vitro model of events associated with wound contraction. Int J Biochem Cell Biol 1997; 29: 121-128. 

  43. Wei D, Zhang L, Williams DL, Browder IW. Glucan stimulates human dermal fibroblast collagen biosynthesis through a nuclear factor-1 dependent mechanism. Wound Repair Regen 2002; 10: 161-168. 

  44. Xu J, Zutter MM, Santoro SA, Clark RA. A three-dimensional collagen lattice activates NF-kappaB in human fibroblasts: role in integrin alpha2 gene expression and tissue remodeling. J Cell Biol 1998; 140: 709-719. 

  45. Yan J, Zong H, Shen A, Chen S, Yin X, Shen X, Liu W, Gu X, Gu J. The beta-(1-->6)-branched beta-(1-->3) glucohexaose and its analogues containing an alpha-(1-->3)-linked bond have similar stimulatory effects on the mouse spleen as Lentinan. Int Immunopharmacol 2003; 3: 1861-1871. 

  46. Zarling JM, Shoyab M, Marquardt H, Hanson MB, Lioubin MN, Todaro GJ. Oncostatin M: a growth regulator produced by differentiated histiocytic lymphoma cells. Proc Natl Acad Sci U S A 1986; 83: 9739-9743. 

저자의 다른 논문 :

섹션별 컨텐츠 바로가기

AI-Helper ※ AI-Helper는 오픈소스 모델을 사용합니다.

AI-Helper 아이콘
AI-Helper
안녕하세요, AI-Helper입니다. 좌측 "선택된 텍스트"에서 텍스트를 선택하여 요약, 번역, 용어설명을 실행하세요.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.

선택된 텍스트