본 연구에서는 감태 효소 추출물과 그것의 폴리페놀 추출물의 화장품 원료로서의 효능을 알아보기 위하여 항산화, 항당화, 미백, 항염 효과와 관련된 실험을 실시하였다. 감태 효소 추출물과 폴리페놀 추출물은 강력한 라디컬 소거능을 가지고 있으며 BSA/Glucose 시스템에서 최종당화생성물의 형성을 저해하는 항당화 활성과 타이로시네이즈 저해를 통한 우수한 미백력을 가지고 있음을 확인하였다. 또한 두 추출물 모두 세포 내에서 $PGE_2$와 NO 생성 저해를 통한 항염 효과를 나타내었다. 이러한 결과를 종합해 볼 때, 감태 효소 추출물과 그 폴리페놀 추출물은 화장품 원료로서의 응용 가능성이 있을 것으로 사료된다.
본 연구에서는 감태 효소 추출물과 그것의 폴리페놀 추출물의 화장품 원료로서의 효능을 알아보기 위하여 항산화, 항당화, 미백, 항염 효과와 관련된 실험을 실시하였다. 감태 효소 추출물과 폴리페놀 추출물은 강력한 라디컬 소거능을 가지고 있으며 BSA/Glucose 시스템에서 최종당화생성물의 형성을 저해하는 항당화 활성과 타이로시네이즈 저해를 통한 우수한 미백력을 가지고 있음을 확인하였다. 또한 두 추출물 모두 세포 내에서 $PGE_2$와 NO 생성 저해를 통한 항염 효과를 나타내었다. 이러한 결과를 종합해 볼 때, 감태 효소 추출물과 그 폴리페놀 추출물은 화장품 원료로서의 응용 가능성이 있을 것으로 사료된다.
To investigate the efficacy of enzymatic extract of Ecklonia cava and its polyphenol extract (AG-DK) as cosmetic ingredients, the anti-oxidative effect, anti-glycation effect, anti-melanogenic effect, and anti-inflammatory effect of the extracts were evaluated in vitro. The enzymatic extract of E. c...
To investigate the efficacy of enzymatic extract of Ecklonia cava and its polyphenol extract (AG-DK) as cosmetic ingredients, the anti-oxidative effect, anti-glycation effect, anti-melanogenic effect, and anti-inflammatory effect of the extracts were evaluated in vitro. The enzymatic extract of E. cava ($SC_{50}$ 42.9 ppm) and AG-DK ($SC_{50}$ 6.4 ppm) showed a strong DPPH free radical scavenging activity. The anti-glycation ability of the enzymatic extract of E. cava and AG-DK was tested using bovine serum albumin (BSA), which inhibited the formation of advanced glycation end-products (AGEs) in the BSA/glucose system. The enzymatic extract of E. cava ($IC_{50}$ 97.2 ppm) and AG-DK ($IC_{50}$ 7 ppm) had inhibitory effects on tyrosinase activity. Moreover, the enzymatic extract of E. cava and AG-DK had an anti-inflammatory effect through the inhibition of nitricoxide (NO) and prostaglandin E2 ($PGE_2$). These findings suggest that the enzymatic extract of E. cava and AG-DK can be applied to skin-care products as cosmetic ingredients.
To investigate the efficacy of enzymatic extract of Ecklonia cava and its polyphenol extract (AG-DK) as cosmetic ingredients, the anti-oxidative effect, anti-glycation effect, anti-melanogenic effect, and anti-inflammatory effect of the extracts were evaluated in vitro. The enzymatic extract of E. cava ($SC_{50}$ 42.9 ppm) and AG-DK ($SC_{50}$ 6.4 ppm) showed a strong DPPH free radical scavenging activity. The anti-glycation ability of the enzymatic extract of E. cava and AG-DK was tested using bovine serum albumin (BSA), which inhibited the formation of advanced glycation end-products (AGEs) in the BSA/glucose system. The enzymatic extract of E. cava ($IC_{50}$ 97.2 ppm) and AG-DK ($IC_{50}$ 7 ppm) had inhibitory effects on tyrosinase activity. Moreover, the enzymatic extract of E. cava and AG-DK had an anti-inflammatory effect through the inhibition of nitricoxide (NO) and prostaglandin E2 ($PGE_2$). These findings suggest that the enzymatic extract of E. cava and AG-DK can be applied to skin-care products as cosmetic ingredients.
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제안 방법
Anti-oxidant activity of the enzymatic extract of E. cava and AG-DK was determined by measuring free radical scavenging activity using the DPPH test. The E.
cava was collected from the shores of Jeju Island in Korea. In this study, E. cava was enzymatically hydrolyzed to prepare water-soluble extracts using carbohydrate enzyme (celluclast 1.5 L FG, Novozyme Nordisk, Bagsvaerd, Denmark), and its polyphenol extract was isolated from enzymatic extract. To briefly state the preparation procedure, 50 g of E.
Interestingly, this technique gains high bioactive compound yield and shows enhanced biological activity in comparison with water and organic extract counterparts. In this study, we evaluated various biological activities of the E. cava enzymatic extract and its polyphenol extract.
The enzymatic extract of E. cava and AG-DK were treated with RAW 264.7 cells to determine their cytotoxic effect, and cell viability was determined using MTT assay. Figure 4 shows that they were not cytotoxic at RAW 264.
To investigate the anti-inflammatory effect of the enzymatic extract of E. cava and AG-DK in RAW 264.7 cells, the inhibition of LPS-induced NO and PGE2 production were evaluated. NO and PGE2 production were greatly increased after LPS treatment for 24 h.
데이터처리
Statistical differences between two groups were determined by a two-tailed Student’s t-test.
참고문헌 (21)
V. K. Dhargalkar and X. N. Verlecar, Southern Ocean seaweeds: A resource for exploration in food and drugs, Aquaculture, 287, 229 (2009).
S. J. Heo, P. J. Park, E. J. Park, S. K. Cho, S. K. Kim, and Y. J. Jeon, Antioxidative effect of proteolytic hydrolysates from Ecklonia cava on radical scavenging using ESR and $H_2O_2$ -induced DNA damage, Food Sci. Biotechnol., 14, 614 (2005).
Y. Athukorala, W. K. Jung, T. Vasanthan, and Y. J. Jeon, Anticoagulative polysaccharide froman enzymatic hydrolysate of Ecklonia cava, Carbohydr. Polym, 66, 184 (2006).
K. N. Kim, K. W. Lee, C. B. Song, and Y. J. Jeon, Cytotoxic activity of green and brown seaweeds collected from Jeju Island against four tumor cell lines. J. Food Sci. Nutr., 11, 17 (2006).
M. M. Kim, Q. V. Ta, E. Mendis, N. Rajapakse, W. K. Jung, H. G. Gyun, Y. J. Jeon, and S. K. Kim, Phlorotannins in Ecklonia cava extract inhibit matrix metalloproteinase activity. Life Sci., 79, 1436 (2006).
M. J. Ahn, K. D. Yoon, S. Y. Min, J. S. Lee, J. H. Kim, T. G. Kim, S. H. Kim, N. G. Kim, H. Huh, and J. Kim, Inhibition of HIV-1 reverse transcriptase and protease by phlorotannins from the brown alga Ecklonia cava. Biol. Pharm. Bull., 27, 544 (2004).
K. A. Kang, K. H. Lee, S. Chae, Y. S. Koh, B. S. Yoo, J. H. Kim, Y. M. Ham, J. S. Baik, N. H. Lee, and J. W. Hyun, Triphlorethol-A from Ecklonia cava protects V79-4 lung fibroblast against hydrogen peroxide induced cell damage. Free Radic. Res., 39, 883 (2005).
K. A. Kang, K. H. Lee, J. W. Park, N. H. Lee, H. K. Na, Y. J. Surh, H. J. You, M. H. Chung, and J. W. Hyun, Triphlorethol-A induces heme oxygenase-1 via activation of ERK and NF-E2 related factor 2 transcription factor. FEBS Lett., 581, 2000 (2007).
C. S. Kong, J. A. Kim, N. Y. Yoon, and S. K. Kim, Induction of apoptosis by phloroglucinol derivative from Ecklonia cava in MCF-7 human breast cancer cells. Food Chem. Toxicol., 47, 1653 (2009).
K. Matsubara, Y. Matsuura, K. Hori, and K. Miyazawa, An anticoagulant proteoglycan from the marine green alga, Codium pugniformis. J. Appl. Phycol., 12, 9 (2000).
T. Kuda, E. Taniguchi, M. Nishizawa, and Y. Araki, Fate of watersoluble polysaccharides in dried Chorda filum a brown alga during water washing. J. Food Compos. Anal., 15, 3 (2002).
N. Matsuura, T. Aradate, C. Sasaki, H. Kojima, M. Ohara, and J. Hasegawa, Screening system for the Maillard reaction inhibitor from natural product extracts, J. Health Sci., 48, 520 (2002).
T. Mossman, Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays, J. Immunol. Meth., 65, 55 (1983).
K. S. Prabhu, F. Z. Davis, J. B. Stewart, J. T. Thompson, L. M. Sordillo, and C. C. Reddy, Selenium deficiency increases the expression of inducible nitric oxide synthase in RAW 264.7 macrophages: Role of nuclear factor- $\kappa$ B in up-regulation, Biochem. J., 366, 203 (2002).
Y. J. Kim and H. Uyama, Tyrosinase inhibitors from natural and synthetic sources: Structure, inhibition mechanism and perspective for the future. Cell. Mol. Life Sci., 62, 1707 (2005).
P. Aroca, K. Urabe, T. Kobayashi, K. Tsukamoto, and U. Hearing, Melanin biosynthesis patterns following hormonal stimulation. J. Biol. Chem., 268, 25650 (1993).
K. Iozumi, G. E. Hoganson, R. Pennella, M. A. Everett, and B. B. Fuller, Role of tyrosinase as the determinant of pigmentation in cultured human melanocytes free, J. Invest. Dermatol., 100, 806 (1993)
D. Latorre, P. Puddu, P. Valenti, and S. Gessani, Reciprocal Interactions between Lactoferrin and Bacterial Endotoxins and Their Role in the Regulation of the Immune Response, Toxins, 2, 54 (2010).
C. C. Wang, C. S. Choy, Y. H. Liu, K. P. Cheah, J. S. Li, J. T. Wang, W. Y. Yu, C. W. Lin, H. W. Cheng, and C. M. Hu, Protective effect of dried safflower petal aqueous extract and its main constituent, carthamus yellow, against lipopolysaccharideinduced inflammation in RAW 264.7 macrophages, J. Sci. Food Agric., 91, 218 (2011).
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