[논문]전탕 압력과 전탕 시간의 차이에 따른 곽향정기산 전탕액 비교

김정훈; 이나리; 신현규; 서창섭

doi:10.14374/hfs.2014.22.2.015

전탕 압력과 전탕 시간의 차이에 따른 곽향정기산 전탕액 비교
Investigation of difference of Gwakhyangjeonggi-san decoctions produced by different pressure levels and various extraction times 원문보기

大韓韓醫學方劑學會誌 = Herbal formula science, v.22 no.2, 2014년, pp.15 - 24

김정훈 (한국한의학연구원 한약방제연구그룹) , 이나리 (한국한의학연구원 한약방제연구그룹) , 신현규 (한국한의학연구원 한약방제연구그룹) , 서창섭 (한국한의학연구원 한약방제연구그룹)

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Objectives : Gwakhyangjeonggi-san (GJS) which consists of 13 herbal medicines has been used to treat gastrointestinal disorders caused by common cold. This study was performed to compare GJS decoctions produced using different pressure levels for various extraction times. Methods : Decoctions were prepared by the pressure levels of $0kgf/cm^2$ (non-pressurized) or $1kgf/cm^2$ (pressurized) for 30-180 min. The extraction yield, total soluble solid content (TSSC), and hydrogen ion concentration (pH) were measured, and the contents of the nine marker compounds were determined using high performance liquid chromatography. Results : The higher pressure and longer extraction time significantly increased TSSC value, while decreased the pH value. However, only extraction time affected the extraction yield of pressurized decoction. Variation of the amounts of chemical compounds was shown in pressurized and non-pressurized decoctions during extraction time. The result of regression analysis showed that pressure and extraction time can influence to extraction yield, TSSC, pH, and the content of chemical compounds. Conclusions : This study suggests that the pressure and extraction time can significantly affect the extraction efficiency of components from GJS decoctions.

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가설 설정

1. A longer extraction time, not pressure, positively affected the extract yield of GJS decoction.
2. Higher pressure and longer extraction time significantly influenced total soluble solid content (TSSC) and the hydrogen ion concentration (pH) of GJS decoction.

제안 방법

The quantification of GJS decoction was performed using high performance liquid chromatography coupled with photodiode array detector. The regression analysis was performed to investigate the influence of extraction factors (pressure and extraction time) on extraction variables, such as extraction yield, TSSC, pH, or the content of each chemical compound.

대상 데이터

1. Chemical structures of 9 standard compounds in Gwakhyangjeonggi-san (GJS).
(Phillipsburg, NJ, USA). Chlorogenic acid and rosmarinic acid were purchased from Sigma-Aldrich (St Louis, MO, USA). Caffeic acid and hesperidin were purchased from Acros Organics (Morris, NJ, USA).
Caffeic acid and hesperidin were purchased from Acros Organics (Morris, NJ, USA). Liquiritin were obtained from NPC Biotechnology (Geumsan, Chungnam, Korea). Apigetrin, oxypeucedanin hydrate, and byakangelicin were supplied from Chengdu Biopurify Phytochemicals Ltd (Chengdu, China), and glycyrrhizin was purchased from Wako Pure Chemical Industries Ltd (Osaka, Japan).
The HPLC-PDA system comprised a Shimadzu LC-20A (Shimadzu Corporation, Kyoto, Japan) equipped with a solvent delivery unit (LC-20AT), autosampler (SIL-20AC), column oven (CTO-20A), degasser (DGU-20A₃), and PDA (SPD-M20A). The acquired data were processed using LabSolutions software (Ver.
The herbal medicines were purchased from the herbal medicine company Kwangmyungdang Medicinal Herbs (Ulsan, Korea) (Table 1). A voucher specimen (2014-KE32-1–13) has been deposited in the Herbal Medicine Formulation Research Group of the Korea Institute of Oriental Medicine.

데이터처리

The influence of pressure and extraction time on the variables, such as extraction yield, TSSC, pH, and the content of each compound, was investigated by regression analysis.
Two-tailed t-tests and Dunnett’s test were conducted for the two-group and the multi-group comparisons using Microsoft Excel (Microsoft, Redmond, WA, USA) and SYSTAT 10 (SPSS Inc., Chicago, IL, USA).

이론/모형

Linear equation, correlation coefficients (r²), LOD, and LOQ in previous study (Kim et al. Nat Prod Commun. 2014) were applied to this study.

성능/효과

These results indicate that the amounts of chemical compounds from aerial parts of herbal medicines showed decreasing patterns with increasing extract time and non-pressurized method produced higher amounts of those compounds, while the amounts of the compounds from roots generally showed increasing patterns and non-pressurized method also produced higher amounts with longer extraction time.
However, the extraction yield of pressurized decoction was increased with longer extraction time. Unlike the difference between extraction yields of decoctions, TSSC was significantly higher in the decoction produced by pressurized method after initial extraction time, 30 min, and it was increased as extraction time increased. The difference of pH between the pressurized and non-pressurized decoctions was also significant after 30 min, showing that pH of GJS decoction was higher when extracted with non-pressurized method (Fig.

참고문헌 (17)

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상세보기
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상세보기
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전탕 압력과 전탕 시간의 차이에 따른 곽향정기산 전탕액 비교
Investigation of difference of Gwakhyangjeonggi-san decoctions produced by different pressure levels and various extraction times 원문보기

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전탕 압력과 전탕 시간의 차이에 따른 곽향정기산 전탕액 비교 Investigation of difference of Gwakhyangjeonggi-san decoctions produced by different pressure levels and various extraction times 원문보기

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가설 설정

제안 방법

대상 데이터

데이터처리

이론/모형

성능/효과

참고문헌 (17)

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저자의 다른 논문 :

김정훈 (63) 이나리 (7) 신현규 (215) 서창섭 (112)

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전탕 압력과 전탕 시간의 차이에 따른 곽향정기산 전탕액 비교
Investigation of difference of Gwakhyangjeonggi-san decoctions produced by different pressure levels and various extraction times 원문보기

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