생체이온 변화 유발 후 경혈과 비경혈에서의 생체 구조 성분 분석 및 비교를 통한 경혈 특이성 고찰 Body Composition Factor Comparisons of the Intracellular Fluid(ICW), Extracellular Fluid(ECW) and Cell Membrane at Acupuncture Points and Non-Acupuncture Points by Inducing Multiple Ionic Changes원문보기
목적 : 경혈의 임피던스를 측정하여 경혈의 특이성을 확보하고자 다수 연구가 진행되어왔다. 직류전압과 교류전압을 자극하여 단순히 경혈이 위치한 피부 임피던스를 측정하는 방식이 아닌 Multi-Frequency Body Impedance analysis(MF BIA) 기법을 이용하여 생체 구조 성분(세포 외액, 세포내액의 저항성분 그리고 세포막의 용량성분)을 추출하는 방법을 이용하여 경혈의 특이성을 확보하고자 한다. 인체 내 생체 이온 변화가 발생하였을 시, 경혈이 비경혈에 발생 전/후 높은 변화율이 관찰될 것이라는 가정을 하에, 생체 이온 변화를 유도하기 위하여 근피로를 유발하였으며, 유도 전/후의 생체 구조 성분을 비교 분석하였다. 방법: 대퇴직근에 근피로를 유도하기 위하여 건강한 대학생에게 Knee extension/flexion의 등속도 운동을 통하였다. 생체 이온 변화를 확인하기 위하여 젖산을 측정하였으며, 피험자마다 동일한 근피로를 유발하기 위하여 EMG(electromyogram) 분석을 통하여 peak torque와 median frequency를 분석하였다. 근피로 유발 24시간 이후까지 젖산과 peak torque와 median frequency을 측정하였으며, 각 단계마다 복토(ST32), 음시(ST33) 과 인접한 비경혈 2개에 대하여 생체 구조 성분 또한 측정하였다. 결과 : 젖산과 peak torque와 median frequency은 24시간 이후 근피로 유발 전으로 회복되었다. 세포외액 저항성분의 경우 비경혈에 비하여 복토(ST32)에서 생체 이온 변화에 따라 높은 변화율이 관찰되었으나, 음시(ST33) 에서는 비경혈에 비하여 낮은 변화율이 관찰되었다. 세포내액 저항성분은 경혈과 비경혈 사이 유의한 차이가 관찰되지 않았다. 복토(ST32)에서 세포막의 용량성분이 높은 변화율이 관찰되었지만, 음시(ST33)와 인접한 비경혈간의 뚜렷한 차이가 확인되지 않았다. 결론 : 생체 이온 변화에 따라 인접한 비경혈과 비교해보았을 시, 경혈에서의 상대적으로 높고 낮은 혹은 유사한 변화율이 관찰되었다. 따라서 경혈의 특이성을 확보하지 못하였으며, 생체 구조 성분 추출을 통하여 세포 이온 변화에 따른 경혈의 특이성을 확보하기에는 한계점을 가지고 있다고 결론을 내렸다.
목적 : 경혈의 임피던스를 측정하여 경혈의 특이성을 확보하고자 다수 연구가 진행되어왔다. 직류전압과 교류전압을 자극하여 단순히 경혈이 위치한 피부 임피던스를 측정하는 방식이 아닌 Multi-Frequency Body Impedance analysis(MF BIA) 기법을 이용하여 생체 구조 성분(세포 외액, 세포내액의 저항성분 그리고 세포막의 용량성분)을 추출하는 방법을 이용하여 경혈의 특이성을 확보하고자 한다. 인체 내 생체 이온 변화가 발생하였을 시, 경혈이 비경혈에 발생 전/후 높은 변화율이 관찰될 것이라는 가정을 하에, 생체 이온 변화를 유도하기 위하여 근피로를 유발하였으며, 유도 전/후의 생체 구조 성분을 비교 분석하였다. 방법: 대퇴직근에 근피로를 유도하기 위하여 건강한 대학생에게 Knee extension/flexion의 등속도 운동을 통하였다. 생체 이온 변화를 확인하기 위하여 젖산을 측정하였으며, 피험자마다 동일한 근피로를 유발하기 위하여 EMG(electromyogram) 분석을 통하여 peak torque와 median frequency를 분석하였다. 근피로 유발 24시간 이후까지 젖산과 peak torque와 median frequency을 측정하였으며, 각 단계마다 복토(ST32), 음시(ST33) 과 인접한 비경혈 2개에 대하여 생체 구조 성분 또한 측정하였다. 결과 : 젖산과 peak torque와 median frequency은 24시간 이후 근피로 유발 전으로 회복되었다. 세포외액 저항성분의 경우 비경혈에 비하여 복토(ST32)에서 생체 이온 변화에 따라 높은 변화율이 관찰되었으나, 음시(ST33) 에서는 비경혈에 비하여 낮은 변화율이 관찰되었다. 세포내액 저항성분은 경혈과 비경혈 사이 유의한 차이가 관찰되지 않았다. 복토(ST32)에서 세포막의 용량성분이 높은 변화율이 관찰되었지만, 음시(ST33)와 인접한 비경혈간의 뚜렷한 차이가 확인되지 않았다. 결론 : 생체 이온 변화에 따라 인접한 비경혈과 비교해보았을 시, 경혈에서의 상대적으로 높고 낮은 혹은 유사한 변화율이 관찰되었다. 따라서 경혈의 특이성을 확보하지 못하였으며, 생체 구조 성분 추출을 통하여 세포 이온 변화에 따른 경혈의 특이성을 확보하기에는 한계점을 가지고 있다고 결론을 내렸다.
Objectives : The specificity of acupuncture point has been a highly controversial subject. Existing researches said that ion-distribution differences are observed on the acupuncture point. This study was conducted under the assumption that multiple ionic changes induced by muscle fatigue would be di...
Objectives : The specificity of acupuncture point has been a highly controversial subject. Existing researches said that ion-distribution differences are observed on the acupuncture point. This study was conducted under the assumption that multiple ionic changes induced by muscle fatigue would be different between the acupuncture point with non-acupuncture point. Methods : To induce the identical fatigue, twenty subjects performed the knee extension/flexion exercise using the Biodex System 3. ST32 and ST33 as well as adjacent non-acupuncture points were selected. We measured blood lactate and analyzed the median frequency(MF) and peak torque. To obtain the information on the extracellular fluid(ECW), intracellular fluid(ICW) and cell membrane indirectly, we used the multi-frequency bioelectrical impedance analysis(MF-BIA) method. Results : MF, peak torque and blood lactate level of all measurement sites were gradually returned to normal. Re resistance of ST32 had a stronger response, but a non-acupuncture point adjacent to ST33 had a larger response up to 20 minutes post exercise. Ri resistances were similar for both acupoints and non-acupoints. The $C_m$ capacitance of ST32 had a stronger response after inducing fatigue, but ST33 had a smaller response than a non-acupuncture point adjacent to it. Conclusions : In comparison with before and after inducing fatigue, the specificity of acupuncture points was not clearly observed. Hence, we concluded that the body composition factors extraction method had the limitation as a method of finding the specificity of acupuncture points by inducing fatigue.
Objectives : The specificity of acupuncture point has been a highly controversial subject. Existing researches said that ion-distribution differences are observed on the acupuncture point. This study was conducted under the assumption that multiple ionic changes induced by muscle fatigue would be different between the acupuncture point with non-acupuncture point. Methods : To induce the identical fatigue, twenty subjects performed the knee extension/flexion exercise using the Biodex System 3. ST32 and ST33 as well as adjacent non-acupuncture points were selected. We measured blood lactate and analyzed the median frequency(MF) and peak torque. To obtain the information on the extracellular fluid(ECW), intracellular fluid(ICW) and cell membrane indirectly, we used the multi-frequency bioelectrical impedance analysis(MF-BIA) method. Results : MF, peak torque and blood lactate level of all measurement sites were gradually returned to normal. Re resistance of ST32 had a stronger response, but a non-acupuncture point adjacent to ST33 had a larger response up to 20 minutes post exercise. Ri resistances were similar for both acupoints and non-acupoints. The $C_m$ capacitance of ST32 had a stronger response after inducing fatigue, but ST33 had a smaller response than a non-acupuncture point adjacent to it. Conclusions : In comparison with before and after inducing fatigue, the specificity of acupuncture points was not clearly observed. Hence, we concluded that the body composition factors extraction method had the limitation as a method of finding the specificity of acupuncture points by inducing fatigue.
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문제 정의
The purpose of this study was to distinguish the difference between acupuncture points and non-acupuncture points in order to determine the therapeutic significance and application of acupuncture points. We generated muscle fatigue in the quadriceps after strenuous knee extension/flexion exercises to induce multiple ionic changes in gradients at acupuncture points or non-acupuncture point locations.
Therefore, it is necessary to approach the new research method to confirm the difference between acupuncture points and non-acupuncture points. This study conducted a comparative experiment on body composition at both acupuncture points and non-acupuncture points upon inducing fatigue. When fatigue occurs, the blood lactate is strongly affected by multiple ionic changes generated across the sarcolemma, transverse(t-) and tubular membranes.
Provided that an acupuncture point responds more quickly and dramatically than a nonacupuncture point, a stronger correlation between changes in body composition factors and blood lactate levels should be observed at an acupuncture point than at a non-acupuncture point. Thus, this study sought to distinguish the differences between acupuncture points and non-acupuncture points.
제안 방법
Twenty participants participated in six consecutive testing sessions. In the first session, twenty participants performed the knee extension/flexion exercise using the Biodex System 3(Biodex Medical Inc., Shirley, NY, USA) to familiarize the participants with the movement requirements. The preexercise was repeated for 10 sets to measure the maximal voluntary contraction(MVC).
In the fourth session, all participants repeated the exercise, which included five sets of 10 repetitions of the isokinetic movement at 115° S-1, in a range of 65% of the re-measured MVC, with 2 minutes of rest between sets.
The preexercise was repeated for 10 sets to measure the maximal voluntary contraction(MVC). In the second session, all participants performed the knee extension/flexion exercise, which is a form of a full-range isokinetic exercise, at 115o S-1 and in a range of 30% of the measured MVC. This exercise was repeated 10 times in a set.
It has also been reported that high frequency bands from 100 kHz to 200 kHz allow for the assessment of both the ICW and ECW by disrupting the cell membrane insulation properties. In this study, we used a frequency band of 5 kHz to assess the ECW, 50 kHz to assess the cell membrane, and 200 kHz to assess the amount of ICW and ECW. Fig.
Thus, the validity of significant therapeutic points remains controversial. Therefore, it is necessary to approach the new research method to confirm the difference between acupuncture points and non-acupuncture points. This study conducted a comparative experiment on body composition at both acupuncture points and non-acupuncture points upon inducing fatigue.
대상 데이터
2 cm in the transverse direction. All measurement sites were located on the rectus femoris. We used two circular silver/silver chloride(Ag/AgCl) surface electrodes( Noraxon Inc.
Twenty males(ages: 23±5.3 years, height: 174±8.7 cm, weight: 76±10.6 kg) participated in this experiment.
5 cm. Twenty participants participated in six consecutive testing sessions. In the first session, twenty participants performed the knee extension/flexion exercise using the Biodex System 3(Biodex Medical Inc.
데이터처리
5, the peak torque decreased after the knee extension/flexion exercise. Table 2 indicated the results of the contrast test based on the one-way repeated measures ANOVA on peak torque. We observed that the peak torque immediately post-exercise was significantly different(p=0.
7 shows the changing Re resistance patterns on all measurement sites. Table 4 indicated the results of the contrast test based on the one-way repeated measures ANOVA on Re resistances. The Re resistances on all measurement sites had increased at 20 minutes post-exercise.
rogram(IBM ctd, USA) for analysis. The contrast test based on the one-way repeated measures analysis of variance (ANOVA) was conducted with each dependent variable(MF, peak torque) obtained by EMG and blood lactate level to determine the recovery time. We also conducted the contrast test based on the one-way repeated measures analysis of variance(ANOVA) to assess the recovery time associated with each dependent variable(Ri, Re, Cm) at all acupuncture points and non-acupuncture points.
The contrast test based on the one-way repeated measures analysis of variance (ANOVA) was conducted with each dependent variable(MF, peak torque) obtained by EMG and blood lactate level to determine the recovery time. We also conducted the contrast test based on the one-way repeated measures analysis of variance(ANOVA) to assess the recovery time associated with each dependent variable(Ri, Re, Cm) at all acupuncture points and non-acupuncture points. The significance level of the statistical analysis was set at p＜0.
이론/모형
Therefore, we selected the multifrequency bioelectrical impedance analysis(MF-BIA) method that penetrates the human body with an alternating current of 800μA, using a frequency band range that varied from 1 kHz to 1 MHz.
성능/효과
05). After 24 hours, there were no significant differences between the ST32 and a non-acupuncture point adjacent to ST32(p＜0.05), but the other points were significantly different(p＜0.05).
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