대추 및 포도를 이용하여 고부가가치성 소재 개발 및 특화작물로서 개발하기 위하여 이들을 이용한 알코올 발효와 초산 발효를 한 후 발효물들의 생리활성을 검증하였다. 항산화 활성을 측정결과 초산 발효물보다 알코올 발효물이 우수한 활성을 나타내었으며, 그 중 대추의 알코올 발효물이 다른 발효물보다 높은 항산화 활성을 나타내었다. 미백효과 측정결과 또한 대추의 알코올 발효물이 우수한 효능을 나타내었으나, immunofluorecence를 이용한 미백효과측정결과 대추의 초산 발효물이 미백에 관련된 단백질 발현을 저해하는 것으로 나타내었다. 대추 및 포도의 발효물들은 대식세포에서 lipopolysaccharide에 의해 생성된 nitric oxide를 농도 의존적으로 감소시켰으며, 알코올 발효물이 초산 발효물보다 우수한 효능을 나타내었다. 발효물들의 항균력을 측정한 결과 포도의 초산 발효물이 Propionibacterium acnes를 제외한 모든 균주에 항균력을 나타내었으며, Escherichia coli에 대해서는 포도의 알코올 발효물과 대추의 알코올 발효물이 높은 항균력을 나타내었다.
For the development of high value consumables utilizing jujube and grape, we investigated the biological activities of a variety of existing fermentation products of jujube and grape. The results revealed that ethanol fermentation products of jujube and grape had a higher antioxidative activity than acetic acid fermentation products. In addition, the ethanol fermentation products of jujube (JEF) had the highest antioxidative activity, with it being greater than that of the ethanol fermentation products of grape (GEF), the acetic acid fermentation products of jujube (JAF) and the acetic acid fermentation products of grape (GAF). As regards tests on whitening effects, JEF exhibited the highest tyrosinase inhibition effects amongst the test groups. However, when immunofluorecence was employed, JAF was seen to inhibit the expression of proteins related to the whitening effect. In the lipopolysaccharide-stimulated mode peritoneal macrophage model, all tested groups of fermentation products (JEF, GEF, JAF and GAF) suppressed nitric oxide production dose-dependently, with ethanol fermentation products demonstrating a higher nitric oxide expression inhibition effect than acetic acid fermentation products. When subjected to antibacterial activity tests, GAF exhibited antibacterial activity against all tested strains except Propionibacterium acnes. Both GAF and JEF revealed high antibacterial activity against Escherichia coli.
Park JG, Kramer BS, Steinberg SM, Carmichael J, Collins JM, Minna JD, et al. 1987. Chemosensitivity testing of human colorectal carcinoma cell lines using a tetrazolium-based colorime- tric assay. Cancer Res. 47: 5875-5879.
Okamura N, Nohara T, Yagi A, Nishioka I. 1981. Studies of dammarane-type saponin of Zizyphus fructus. Chem. Pharm. Bull. 29: 675-683.
Palma M, Taylor LT. 1999. Extraction of polyphenolic compounds from grape seeds with near critical carbon dioxide. J. Chromatogr. A. 849: 117-124.
Piver B, Berthou F, Dreano Y, Lucas D. 2001. Inhibition of CYP3A, CYP1A and CYP2E1 activities by resveratrol and other non volatile red wine components. Toxicol. Lett. 125: 83-91.
Pryor WA. 1986. Oxy-radicals and related species : their formation, lifetimes, and reactions. Annu. Rev. Physiol. 48: 657-667.
Rhee YK, Kim DH, Han MJ. 1998. Inhibitory effect of Zizyhi frutus on glucuronidase and tryptophanase of human intesteria. Korean J. Food Sci. Technol. 30: 199-205.
Roberta R, Nicoletta P, Anna P, Ananth P, Min Y, Catherine RE. 1999. Antioxidant acitivity applying an improved ABTS radical cation decolorization assay. Free Rad. Biol. Med. 26: 1231-1237.
Saha B, Singh SK, Sarkar C, Bera R, Ratha J, Tobin DJ, et al. 2006. Activation of the Mitf promoter by lipid-stimulated activation of p38-stress signalling to CREB. Pigm. Cell Res. 19: 595-605.
Sassone-Corsi P. 1998. Coupling gene expression to cAMP signalling: role of CREB and CREM. Int. J. Biochem. Cell Biol. 30: 27-38.
Yagi A, Kanbara T, Morinobu N. 1986. The effect of tyrosinase inhibition for aloe. Planta Medica. 3981: 517-519.
Shirataki Y, Kawase M, Saito S, Kurihara T, Tanaka W, Satoh K, et al. 2000. Selective cytotoxic activity of grape peel and seed extracts against oral tumor cell lines. Anticancer Res. 20: 423-426.
Shon MY. 2007. Antioxidant and anticancer activities of Poria cocos and Machilus thunbergii fermented with Mycelial Mushrooms. Food Ind. Nutr. 12: 51-57.
Wang MF, Shao Y, Li JG, Zhu NQ, Rngarajan M, LaVoie EJ, et al. 1999. Antioxidative phenolic compound from sage (Salivia officinalis). J. Agric. Food Chem. 62: 454-456.
Yamaguchi N. 1969. Studies on the browning reaction products from reducing sugars and amino acids, Part IX. Antioxidative activities of browning reaction products and several reductones. J. Japanese Soc. Food Sci. Technol. 16: 140-144.
Zhao J, Wang J, Chen Y, Agarwal R. 1999. Anti-tumor-promoting activity of a polyphenolic fraction isolated from grape seeds in the mouse skin two-stage initiation-promotion protocol and identification of procyanidin B5-3'-gallate as the most effective antioxidant constituent. Carcinogenesis 20: 1737- 1745.
Zryaev R, Irgasheve T, Israilov IA, Abdullaev ND, Yunusov MS, Yunusov S. 1977. Alkaloids of Zizyphus jujuba structure of yuziphine and yuzirine. Khim. Prir. Soedin. 2: 239-243.
Bagchi D, Garg A, Krohn RL, Bagchi M, Tran MX, Stohs SJ. 1997. Oxygen free radical scavenging abilities of vitamins C and E, and a grape seed proanthocyanidin extract in vitro. Res. Commun. Mol. Pathol. Pharmacol. 95: 179-189.
Bal JS, Jawanoda JS, Singh SN. 1979. Development physiology of ber (Zizyphus mauritina) var. urman. IV. Change in amino acids and sugar (sucrose, glucose and fructose) at different stages of fruit ripening. Ind. Food. Pack. 33: 3335-3337.
Conner DE, Beuchat LR. 1984. Sensitivity of heat-stressed yeasts to essential oils of plants. Appl. Environ. Microbiol. 47: 229-233.
Blois MS. 1958. Antioxidant determination by the use of a stable free radical. Nature. 26: 1199-1120.
Busca R, Ballotti R. 2000. Cyclic AMP a key messenger in the regulation of skin pigmentation. Pigm. Cell Res. 13: 60-90.
Carmichael J, DeGraff WG, Gazdar AF, Minna JD, Mitchell JB. 1987. Evaluation of a tetrazolium-based semiautomated colorimetric assay: assessment of chemosensitivity testing. Cancer Res. 47: 936-942.
Han HS, Park JH, Choi HJ, Son JH, Kim YH, Kim S, et al. 2004. Biochemical analysis and physiological activity of perilla leaves. Korean J. Food Culture. 19: 94-105.
Hong JY, Nam HS, Shin SR. 2010. Changes on the antioxidant activities of extracts from the Ziziphus jujube Miller Fruits during maturation. Korean J. Food Preserv. 17: 712-719.
Imokawa G, Mishima Y. 1980. Isolation and characterization of tyrosinase inhibitors and their differential action on melanogenic subcellular compartments in amelanotic and melanomas. Br. Japan Dermatol. 103: 625-633.
Imokawa G, Mishima Y. 1981. Biochemical characterization of tyrosinase inhibitors using tyrosinase binding affinity chromatography. Br. Japan Dermatol. 104: 531-539.
Kameyama KC, Sakai C, Kuge S, Nishiyama S, Tomita Y, Ito S, et al. 1995. The expression of tyrosinase, tyrosinaserelated proteins 1 and 2 (TRP 1 and TRP 2), the silver protein, and a melanogenic inhibitor in human melanoma cells of differing melanogenic activies. Pigm. Cell Res. 8: 97-104.
Kim WS. 1995. Grape processing industries. In New Cultivation Method of Grape, pp. 58-92. Munun Publishing Co, Seoul.
Krigaya N, Kato H, Fujimaki M. 1971. Studieson antioxidant activity of nonenzymic browning reaction products, Part III. Fractionation of browning reaction solution between ammonia and D-glucose and antioxidant activity of the resulting fractions. J. Agric. Chem. Soc. Japan 45: 292-298.
Kwon OW, Kim CH, Kim HS, Kwon MC, Ahn JH, Lee HJ, et al. 2007. Comparison of immuno modulatary and anticancer activities according to the parts of the Styrax japonica Sieb. et Zucc. Korean J. Med. Crop Sci. 15: 170-176.
Marklund S, Marklund G. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47: 469-474.