[논문]CLA 첨가사료가 감성돔(Acanthopagrus schlegeli) 성장과 지방산 조성 및 내장 추출지방이 지방세포 3T3-L1에 미치는 영향

곽뢰; 쥴리야티 로마; 최광수; 박시향; 하영래; 강석중; 최병대

doi:10.5352/jls.2015.25.5.548

CLA 첨가사료가 감성돔(Acanthopagrus schlegeli) 성장과 지방산 조성 및 내장 추출지방이 지방세포 3T3-L1에 미치는 영향
Influence of Dietary Conjugated Linoleic Acid (CLA) and Carotenoids on Growth, Fatty Acid Composition, and 3T3-L1 Cells in Black Seabream (Acanthopagrus schlegeli) 원문보기

생명과학회지 = Journal of life science, v.25 no.5 = no.181, 2015년, pp.548 - 556

곽뢰 (경상대학교 해양식품생명의학과) , 쥴리야티 로마 (경상대학교 해양식품생명의학과) , 최광수 (경상대학교 해양환경공학과) , 박시향 (선마린바이오) , 하영래 (경상 대학교 환경생명화학과) , 강석중 (경상대학교 해양식품생명의학과) , 최병대 (경상대학교 해양식품생명의학과)

초록
AI-Helper

공액리놀레산(CLA)과 카로테노이드 일정량을 함유하는 두 가지 사료구와 대조구를 감성돔(Acanthopagrus schlegeli)에 급여하였다. 대조구에는 CLA와 카로테노이드가 포함되지 않았고, CP10구에는 CLA 1%와 카로테노이드 0.1%가 포함되었고, CP25구에는 CLA 2.5%와 카로테노이드 0.1%가 포함되었다. 대조구, CP10 및 CP25구의 일일성장률(SGR)은 0.74, 0.81, 0.97이었고, 사료요구율은 2.65, 2.46 및 2.04이었다. 사육 후 근육 내에 축적된 CLA 함량은 CP10 및 CP25구에서 각각 2.8% 및 5.6%였으며, 내장에 축적된 CLA 함량은 4.0% 및 8.3%였다. 한편, CP25구의 에서 추출된 지용성 물질(VLE)을 첨가한 3T3-L1 지방세포 생존율은 대조구와 비교하였을 때 처리량에 따라 영향을 받는 것으로 나타났다. 따라서 CP25구의 VLE 추출물 농도를 1, 10, 50 및 $100{\mu}g/ml$으로 처리하였을때 각각 $85.9{\pm}0.8%$, $74.8{\pm}0.9%$, $75.5{\pm}0.6%$ 및 $72.1{\pm}0.7%$ 감소시켰다. 이와 같은 사료로 감성돔을 사육하는 경우 CP10 및 CP25구의 내장에서 추출한 지질함량 중 CLA함량은 각각 4.0% 및 8.5%, 카로테노이드는 각각 0.62 mg/g 및 0.56 mg/g, EPA 및 DHA는 각각 11.7%와 10.8% 및 11.8%와 12.7%로 증가하였고, 총 PUFA 함량도 각각 48.4% 및 50.5%로 증가하여 인체에 긍정적인 영향을 미치는 성분을 동시에 섭취할 수 있어 기능성 어류의 생산이 가능한 것으로 나타났다.

Abstract ▼ AI-Helper

Three groups of black seabream (Acanthopagrus schlegeli) were fed with treatment diets containing certain concentrations of conjugated linoleic acid (CLA) and carotenoids. The control group feed contained 0% CLA and 0% carotenoids, the CP10 group feed contained 1% CLA and 0.1% carotenoids, and the CP25 group feed contained 2.5% CLA and 0.1% carotenoids. The CP10 and CP25 groups demonstrated the enhanced growth and increased feed conversion efficiency of black seabream. The specific growth rates (SGRs) were 0.74, 0.81, and 0.97, while the feed conversion ratios (FCRs) were 2.65, 2.46, and 2.04 for the control, CP10, and CP25 groups, respectively. The total contents of high unsaturated fatty acid (HUFA) for the control, CP10, and CP25 groups were 41.0%, 41.7%, and 43.5%, respectively. CLA was deposited to the extent of 2.8% and 5.6% in the muscle, and 4.0% and 8.3% in the viscera of the CP10 and CP25 groups, respectively. Meanwhile, treatment with the viscera lipid extract (VLE) from CP25 fish evidently lowered 3T3-L1 adipocytes viability. The lipid extract from the muscle and viscera of black seabream contained ample amounts of beneficial substances, such as CLA, carotenoids, EPA, and DHA. CLA, which enriched black seabream muscle, could be categorized as a functional food and serve as a well-being food. Meanwhile, the fish oil from its viscera could serve as a high function supplement.

주제어

AI 본문요약
AI-Helper

* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.

제안 방법

In addition of inducer mixture, 1, 10, 50, 100 μg/ml of black seabream viscera’s lipid extract (VLE) from control and CP25 group were added respectively. After 48 hr in- cubation, the medium was replaced with DMEM plus 10% BCS, 5 μg/ml insulin and VLE of control and CP25 0, 1, 10, 50, 100 μg/ml respectively. Reference group was a cul- ture without addition of VLE.
However, this method has a drawback, since CLA has prominent effect as body fat reducer thus it will lower harvest weight. This study aims are to inves- tigate the influence of dietary conjugated linoleic acid (CLA) in alliance with carotenoids on growth and fatty acid composition in black seabream, and to examine the impact of black seabream lipid extract on 3T3-L1 cells. The addition of carotenoids will feasibly maintain the weight of the fish as well as enhance meat coloring.
size. Treatments were conducted for 8 weeks, feed were given 3% of fish body weight, twice a day at 10:00 am and 17:00 pm until satiation. The experiment was conducted during summer (June-August) in sea area of Tongyeong, Korea.

대상 데이터

1% carotenoids). CLA (purity ≥ 77%) was supplied by HK Biotech Co. (Jinju, Korea), which consisted: 39% cis-9, trans-11 CLA; 39% trans-10, cis-12 CLA; and 2% other CLA isomers (cis-9, cis-11; cis-10, cis-12; trans- 9, trans-11, and trans-10, trans-12 CLA). Commercial car- otenoids that being used in this study was Carophyll Pink^® (contained 10% astaxanthin) obtained from Vixxol Co.
One thousand and five hundred fishes were randomly distributed into three groups (Control, CP10 and CP25) and allotted to cages of 175 m³ (5 m × 5 m × 7 m) in size. Treatments were conducted for 8 weeks, feed were given 3% of fish body weight, twice a day at 10:00 am and 17:00 pm until satiation.
Treatments were conducted for 8 weeks, feed were given 3% of fish body weight, twice a day at 10:00 am and 17:00 pm until satiation. The experiment was conducted during summer (June-August) in sea area of Tongyeong, Korea. Water quality was monitored twice a week at 08:00-09:00 am.

데이터처리

Hepatosomatic index (HI) was equal to viscera weight over body weight times 100. Data were analyzed for the degree of variation and significant differences, based on Analysis of Variance (ANOVA), along with the Tukey’s pair-wise comparison test between treatment means and Duncan’s multiple range test (DMRT) to determine the differences. Analyses were conducted by using the JMP statistical soft- ware (SAS Institute Inc.

이론/모형

hr during differentiation steps. Cells viability was de- termined with MTS assay. Fig.
were analyzed. Protein content was determined with Kjeldahl method [1]. Total Lipid content was analyzed with Bligh and Dyer [4] method.
Total Lipid content was analyzed with Bligh and Dyer [4] method. The moisture and ash of the samples were determined with methods according to AOAC [1]. For fatty acid methyl ester (FAME) analysis, Park et al.
Protein content was determined with Kjeldahl method [1]. Total Lipid content was analyzed with Bligh and Dyer [4] method. The moisture and ash of the samples were determined with methods according to AOAC [1].

성능/효과

In conclusion, the present research demonstrated dietary CLA in corporate with carotenoids will increase CLA de- posit in black seabream without hinder its growth. This diet regime generated higher final weight on black seabream, which are 205.
Result showed that SGR was 0.74, 0.81, and 0.97 and FCR was 2.65, 2.46, and 2.04 for control, CP10, and CP25 re- spectively (Table 2). VSI of treatment groups was sig- nificantly lower from control (p<0.
This study showed that CLA incorporated with car- otenoids on diets could augment PUFA level in black seabream. The percentages of CLA deposited in muscle of CP10 and CP25 feed group were 2.8% and 5.6%, while their levels in viscera were 4.0% and 8.3% respectively. These ex- hibited significant differences (p<0.
The effects of carotenoids on growth performance are consistent with the acknowl- edged relationship between carotenoids signals to growth and health [14]. This study demonstrated that even though the CLA has fat reducing activity, addition of 0.1% car- otenoids would diminish that effect and promoted growth on black seabream.
These were one of the immense problems with dietary CLA for fish. This study showed that CLA incorporated with car- otenoids on diets could augment PUFA level in black seabream. The percentages of CLA deposited in muscle of CP10 and CP25 feed group were 2.

참고문헌 (40)

AOAC International. 2003. Official methods of analysis of AOAC International. 17 th edition, 2nd revision. Gaithersburg, MD, USA, Association of Analytical Communities.
Benitez-Santana, T., Masuda, R., Carrillo, J., Ganuza, E., Valencia, A., Hernandez-Cruz, C. M. and Izquierdo, M. S. 2007. Dietary n-3 HUFA deficiency induces a reduced visual response in gilthead seabream Sparusaurata larvae. Aquaculture 264, 408-417.

상세보기
Berge, G. M., Ruyter, B. and Asgard, T. 2004. Conjugated linoleic acid in diets for juvenile Atlantic salmon (Salmo salar); effects on fish performance, proximate composition, fatty acid and mineral content. Aquaculture 237, 365-380.

상세보기
Bligh, E. G. and Dyer, W. J. 1959. A rapid method of total lipid extraction and purification. Can. J. Biochem. Physiol. 37, 911-917.

상세보기
Breslow, J. L. 2006. Omega-3 fatty acids and cardiovascular disease. Am. J. Clin. Nutr. 83 S1477-S1482.

상세보기
Brown, I., Wahle, K. W., Cascio, M. G., Smoum-Jaouni, R., Mechoulam, R., Pertwee, R. G. and Heys, S. D. 2011. Omega-3 N-acylethanolamines are endogenously synthesised from omega-3 fatty acids in different human prostate and breast cancer cell lines. Prostaglandins Leukot.Essent. Fatty Acids 85, 305-310.

상세보기
Chin, S. F., Liu, W., Storkson, J. M., Ha, Y. L. and Pariza, M. W. 1992. Dietary sources of conjugated dienoic isomers of linoleic acid, a newly recognized class of anticarcinogens. J. Food Composit. Anal. 5, 185-197.

상세보기
Choi, B. D., Kang, S. J., Ha, Y. L. and Ackman, R. G. 1999. Accumulation of conjugated linoleic acid (CLA) in tissues of fish fed diets containing various levels of CLA, pp. 61-71. In: Xiong, Y. L., Ho, C. T. and Shahidi, F. (eds.), Quality Attributes of Muscle Foods. Kluwer Academic/Plenum Publishers, New York, USA.
Cockbain, A. J., Toogood, G. J. and Hull, M. A. 2012. Omega-3 polyunsaturated fatty acids for the treatment and prevention of colorectal cancer. Gut 61, 135-149.

상세보기
Ferrucci, L., Cherubini, A., Bandinelli, S., Bartali, B., Corsi, A., Lauretani, F., Martin, A., Andres-Lacueva, C., Senin, U., Jack, M. and Guralnik, J. M. 2006. Relationship of plasma polyunsaturatedfatty acids to circulating inflammatory markers. J. Clin. Endocrinol. Metab. 91, 439-446.

상세보기
Food and Agriculture Organization (FAO). 2010. World review of fisheries and aquaculture, pp. 3-89. In: FAO Fisheries and Aquaculture Department, (eds.), The State of World Fisheries and Aquaculture. FAO Sales and MarketingGroup, Rome, Italy.
Graham, I. A., Larson, T. and Napier, J. A. 2007. Rational metabolic engineering of transgenic plants for biosynthesis of omega-3 polyunsaturates. Curr. Opin. Biotechnol. 18, 142-147.

상세보기
Gu, Z., Suburu, J., Chen, H. and Chen, Y. Q. 2013. Mechanisms of omega-3 polyunsaturated fatty acids in prostate cancer prevention. Biomed. Res. Int. 2013, 824563.
Güroy, B., Sahin, I., Mantoglu, S. and Kayali, S. 2012. Spirulina as a natural carotenoid source on growth, pigmentation and reproductive performance of yellow tail cichlid Pseudotropheusacei. Aquacult. Int. 20, 869-878.

상세보기
Ha, Y. L., Grimm, N. K. and Pariza, M. W. 1987. Anticarcinogens from fried ground beef: heat-altered derivatives of linoleic acid. Carcinogenesis 8, 1881-1887.

상세보기
Halade, G. V., Rahman, M. M., Williams, P. J. and Fernandes, G. 2010. High fat diet-induced animal model of age-associated obesity and osteoporosis. J. Nutr. Biochem. 21, 1162-1169.

상세보기
Han, C. Y., Kargi, A. Y., Omer, M., Chan, C. K, Wabitsch, M., O’Brien, K. D., Wight, T. N. and Chait, A. 2010. Differential effect of saturated and unsaturated free fatty acids on the generation of monocyte adhesion and chemotactic factors by adipocytes. Diabetes 59, 386-396.

상세보기
Hardy, R. W. and Barrows, F. T. 2002. Diet formulation and manufacturing, pp. 505-600. In: Halver, J. E. and Hardy, R. W. (eds.), Fish Nutrition. Academic Press Inc., New York, USA.
Inoue, M., Tanabe, H., Matsumoto, A., Takagi, M., Umegaki, K., Amagaya, S. and Takahashi, J. 2012. Astaxanthin functions differently as a selective peroxisome proliferator-activated receptor g modulator in adipocytes and macrophages. Biochem. Pharm. 84, 692-700.

상세보기
Kim, M. J., Chang, U. J. and Lee, J. S. 2009. Inhibitory effects of fucoidan in 3T3-L1 adipocyte differentiation. Mar. Biotechnol. 11, 557-562.

상세보기
Kim, H. K., Della-Fera, M. A., Lin, J. and Baile, C. A. 2006. Docosahexaenoic acid inhibits adipocyte differentiation and induces apoptosisin 3T3-L1 preadipocytes. J. Nutr. 136, 2965-2969.

상세보기
Leaver, M. J., Tocher, D. R., Obach, A., Jensen, L., Henderson, R. J., Porter, A. R. and Krey, G. 2006. Effect of dietary conjugated linoleic acid (CLA) on lipid composition, metabolism and gene expression in Atlantic salmon (Salmo salar) tissues. Comp. Biochem. Physiol. 145A, 258-267.
Lee, K. N., Kritchevsky, D. and Pariza, M. W. 1994. Conjugated linoleic acid and atherosclerosis in rabbits. Atherosclerosis 108, 19-25.

상세보기
Lowery, L. M., Appicelli, P. A. and Lemon, P. W. R. 1998. Conjugated linoleic acid enhances muscle size and strength gains in novice body builders. Med. Sci. Sports Exerc. 30, S182.

상세보기
Ostrowska, E., Muralitharan, M., Cross, R. F., Bauman, D. E. and Dunshea, F. R. 1999. Dietary conjugated linoleic acids increase lean tissue and decrease fat deposition in growing pigs. J. Nutr. 129, 2037-2042.

상세보기
Park, S. J., Park, C. W., Kim, S. J., Kim, J. K., Kim, Y. R., Park, K. A., Kim, J. O. and Ha, Y. L. 2002. Methylation methods for the quantitative analysis of conjugated linoleic acid (CLA) isomers in various lipid samples. J. Agric. Food Chem. 50, 989-996.

상세보기
Pischon, T., Hankinson, S. E., Hotamisligil, G. S., Rifai, N., Willett, W. C. and Timm, E. B. 2003. Habitual dietary in takeof n-3 and n-6 fatty acids in relation to inflammatory markers among US men and women. Circulation 108, 155-160.

상세보기
Rodriguez-Amaya, D. B. and Kimura, M. 2004. Harvest Plus Handbook for Carotenoid Analysis. Washington DC, WD, USA.
Sapieha, P., Stah, A., Chen, J., Seaward, M. R., Willett, K. L., Krah, N. M., Dennison, R. J., Connor, K. M., Aderman, C. M., Liclican, E., Carughi, A., Perelman, D., Kanaoka, Y., San Giovanni, J. P., Gronert, K. and Smith, L. E. H. 2011. 5-Lipoxygenase metabolite 4-HDHA is a mediator of the antiangiogenic effect of n-3 polyunsaturated fatty acids. Sci. Transl. Med. 3, 69 ra12.
Simopoulos, A. P. 2008. The omega-6/omega-3 fatty acid ratio, genetic variation, and cardiovascular disease. Asia Pac. J. Clin. Nutr. 17, 131-134.
Tan, X. Y., Luo, Z., Xie, P., Li, X. D., Liu, X. J. and Xi, W. Q. 2010. Effect of dietary conjugated linoleic acid (CLA) on growth performance, body composition and hepatic intermediary metabolism in juvenile yellow catfish Pelteobagrus fulvidraco. Aquaculture 310, 186-191.

상세보기
Trichet, V. V. 2010. Nutrition and immunity: an update. Aquacult. Res. 41, 356-372.

상세보기
Twibell, R. G. and Wilson, R. P. 2003. Effects of dietary conjugated linoleic acids and total dietary lipid concentrations on growth responses of juvenile channel catfish, Ictaluruspuncatus. Aquaculture 221, 621-628.

상세보기
Twibell, R. G., Watkins, B. A., Rogers, L. and Brown, P. B. 2000. Effects of dietary conjugated linoleic acids on hepatic and muscle lipids in hybrid striped bass. Lipids 35, 155-161.

상세보기
Valente, L. M. P., Bandarra, N. M., Figueiredo-Silva, A. C, Rema, P., Vaz-Pires, P., Martins, S., Prates, J. A. M. and Nunes, M. L. 2007. Conjugated linoleic acid in diets for large-size rainbow trout (Oncorhynchus mykiss): effects on growth, chemical composition and sensory attributes. Br. J. Nutr. 97, 289-297.

상세보기
Watras, A. C., Buchholz, A. C., Close, R. N., Zhang, Z. and Schoeller, D. A. 2007. The role of conjugated linoleic acid in reducing body fat and preventing holiday weight gain. Int. J. Obes. 31, 481-487.

상세보기
Wijendran, V. and Hayes, K. C. 2004. Dietary n-6 and n-3 fatty acid balance and cardiovascular health. Annu. Rev. Nutr. 24, 597-615.

상세보기
Williams, C. D., Whitley, B. M., Hoyo, C., Grant, D. J., Iraggi, J. D., Newman, K. A., Gerber, L., Taylor, L. A., McKeever, M. G. and Freedland, S. J. 2011. A high ratio of dietary n-6/n-3 polyunsaturated fatty acids is associated with increased risk of prostate cancer. Nutr. Res. 31, 1-8.

상세보기
Yalpani, M. 2002. New technologies for health foods and nutraceuticals. J. Food Sci. Nutr. 7, 222-229.
Yasmin, A. and Takeuchi, T. 2002. Influence of dietary levels of conjugated linoleic acid (CLA) on juvenile tilapia Oreochromis niloticus. J. Zhejang Univ. Sci. 9B, 691-700.

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

내보내기 구분	파일저장 인쇄 메일전송
구성항목	기본정보 상세정보 관리번호, 논문명, 저널/프로시딩명, 저자 , 발행년, 권, 호, 시작페이지, 끝페이지, 발행기관 관리번호, 논문명, 대등논문명, 저자 , 저널/프로시딩명, 발행기관, 발행년, 발행언어, 권, 호, 시작페이지, 끝페이지, ISBN, ISSN, 주제분야, 키워드, 초록(한글), 초록(영문), 저자(소속기관)
저장형식	Text(ASCII format) Excel format RefWorks Direct Export RIS format (for Reference Manager, ProCite, EndNote), Scholar's Aids, Mendeley
메일정보	받는사람 (필수) @ 보내는사람 (선택) @ 제목 내용 KISTI 검색결과 이메일 서비스
안내	총 건의 자료가 검색되었습니다. 다운받으실 자료의 인덱스를 입력하세요. (1-10,000) 검색결과의 순서대로 최대 10,000건 까지 다운로드가 가능합니다. 데이타가 많을 경우 속도가 느려질 수 있습니다.(최대 2~3분 소요) 다운로드 파일은 UTF-8 형태로 저장됩니다. 파일의 내용이 제대로 보이지 않을실 때는 웹브라우저 상단의 보기 -> 인코딩 -> 자동선택 여부를 확인하십시오. ~ Text(ASCII format) Excel format