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The Effect of GCSB-5 a New Herbal Medicine on Changes in Pain Behavior and Neuroglial Activation in a Rat Model of Lumbar Disc Herniation

Journal of Korean Neurosurgical Society = 대한신경외과학회지, v.59 no.2, 2016년, pp.98 - 105  

Cho, Hee Kyung (Department of Physical Medicine and Rehabilitation, College of Medicine, Catholic University of Daegu) ,  Kim, So-Yeon (Medical Devices Clinical Trial Center, College of Medicine, Yeungnam University) ,  Choi, Mi Jung (Medical Devices Clinical Trial Center, College of Medicine, Yeungnam University) ,  Baek, Seung Ok (Department of Physical Medicine and Rehabilitation, College of Medicine, Yeungnam University) ,  Kwak, Sang Gyu (Department of Medical Statistics, College of Medicine, Catholic University of Daegu) ,  Ahn, Sang Ho (Medical Devices Clinical Trial Center, College of Medicine, Yeungnam University)

Abstract AI-Helper 아이콘AI-Helper

Objective : Lumbar disc herniation can induce sciatica by mechanical compression and/or chemical irritation. The aim of this study was to compare the effects of GCSB-5 (Shinbaro$^{(R)}$) and NSAIDs on pain-related behavior and on the expressions of microglia, astrocytes, CGRP, TRPV1, IL-6...

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제안 방법

  • After nucleus pulposus implantation animals were divided into five treatment groups : a saline group (the vehicle control group) (n=27), a 10 mg/kg aceclofenac group (the aceclofenac group) (n=22), and 100, 300, and 600 mg/kg GCSB-5 groups (the GCSB-5 100, 300, and 600 groups) (n=21 for each group). Drugs were dissolved in 0.
  • Mechanical sensitivities of the plantar surfaces of ipsilateral hind paws were tested at 3 days after surgery and at 1 day, 3 days, 7 days, 14 days, 21 days, 28 days, 35 days, 42 days, 49 days, and 56 days after treatment commencement (Fig. 1). Mechanical allodynia was determined by measuring withdrawal response to mechanical stimulation of ipsilateral hind paws with von Frey filaments (North Coast Medical Inc.
  • This study was undertaken to compare the effects of aceclofenac (a NSAID) and GCSB-5 on pain-related behavior and on the expressions of Ionized calcium-binding adapter molecule 1 (Iba1), Glial fibrillary acidic protein (GFAP), calcitonin gene-related peptide (CGRP), transient receptor potential vanilloid 1 (TRPV1), IL-6, and CX3CL1 in a rat model of lumbar disc herniation.

대상 데이터

  • A total of 112 female Sprague-Dawley rats (200–220 g) were used.
  • The dilutions used were optimal, according to the manufacturers’ recommendations. Immunofluorescent images were acquired using a cooled charge-coupled device (CCD) camera (Olympus DP71, Tokyo, Japan) attached to a light microscope (Olympus BX51, Tokyo, Japan).

데이터처리

  • Paw withdrawal thresholds by time after treatment commencement, group, and interaction effects (time difference by group) were analyzed using repeated measures two factor analysis and multiple comparisons, and the results obtained were compared. One-way ANOVA was used to determine the significances of variable differences between D0 (the day of treatment commencement) and later times, and Scheffe test was used for multiple comparisons. p-values are presented for significant variables.
  • Quantitative variables are presented as means (SDs). Paw withdrawal thresholds by time after treatment commencement, group, and interaction effects (time difference by group) were analyzed using repeated measures two factor analysis and multiple comparisons, and the results obtained were compared. One-way ANOVA was used to determine the significances of variable differences between D0 (the day of treatment commencement) and later times, and Scheffe test was used for multiple comparisons.
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