[논문]STZ으로 유도된 당뇨 마우스에서 오디열매추출물의 식후 고혈당 완화 효과

최경하; 강지혜; 한지숙

doi:10.5352/jls.2016.26.8.921

STZ으로 유도된 당뇨 마우스에서 오디열매추출물의 식후 고혈당 완화 효과
Alleviating Effects of Mulberry Fruit Extract on Postprandial Hyperglycemia in Streptozotocin-induced Diabetic Mice 원문보기

생명과학회지 = Journal of life science, v.26 no.8 = no.196, 2016년, pp.921 - 927

최경하 (부산대학교 식품영양학과신라대학교 식품영양학과) , 강지혜 (부산대학교 식품영양학과신라대학교 식품영양학과) , 한지숙 (부산대학교 식품영양학과신라대학교 식품영양학과)

초록
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식후 고혈당은 제2형 당뇨병 초기 진단과 항당뇨병의 중요한 바이오마커 중 하나이다. α-글루코시다아제 억제제는 소장에서 탄수화물 소화의 속도를 방해함으로써 식후 고혈당을 조절한다. 본 연구에서는 오디열매추출물이 α-글루코시다아제와 α-아밀라아제에 미치는 억제효과 및 스트렙토조토신(STZ)이 유발하는 당뇨병 생쥐의 식후고혈당에 미치는 완화 효과를 조사하였다. α-글루코시다아제와 α-아밀라아제에 대한 오디열매추출물(MFE)의 IC₅₀ 값은 각각 0.16과 0.14 mg/ml의 결과값을 나타내어, 양성대조군인 acarbose보다 더 효과적이었다. STZ으로 유발된 당뇨병 흰쥐의 식후 혈당 수치는 정상 대조군에 비해 오디열매추출물에서 유의적으로 더 낮았다. 더욱이, 오디열매추출물 투여는 당뇨병 흰쥐에서 포도당 반응에 대한 곡선하면적 감소와 관련이 있었다. 결론적으로, 오디열매추출물은 α-글루코시다아제와 α-아밀라아제 활성의 강력한 억제제이며 식후 고혈당을 완화할 수 있다는 내용이다. 따라서 오디열매추출물은 당뇨병 치료제 및 기능성식품의 소재로 가치가 높다고 사료된다.

Abstract ▼ AI-Helper

Postprandial hyperglycemia is an early defect of type 2 diabetes and one of primary anti-diabetic targets. The alpha-glucosidase inhibitors regulate postprandial hyperglycemia by impeding the rate of carbohydrate (such as starch) digestion in the small intestine. This study was designed to investigate the inhibitory actions of mulberry fruit extract (MFE) on α-glucosidase and α-amylase activities, and its alleviating effect on postprandial hyperglycemia activities in vitro and in vivo. Male four-week old ICR mice and streptozotocin (STZ)-induced diabetic mice were treated with mulberry fruit extract. MFE showed strong inhibitory effects against α-glucosidase and α-amylase activities, with half-maximal inhibitory concentration (IC50) values of 0.16 and 0.14 mg/ml, respectively, and was more effective than acarbose, which was used as a positive control. The increase in postprandial blood glucose levels was more significantly attenuated in the MFE-administered group mice than in the control group mice of both STZ-induced diabetic and normal mice. Moreover, the area under the glucose response curve significantly decreased following MFE administration in diabetic mice. These results indicate that MFE may be a potent inhibitor of α-glucosidase and α-amylase, and helpful in suppressing postprandial hyperglycemia in diabetic mice. The mulberry fruit extracts may be considered as a potential candidate for the management of diabetes.

주제어

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가설 설정

Therefore, maintaining near-normal levels of glycemic control, both in the fasting and postprandial states, is a treatment goal for patients with type 2 diabetes [27]. In this study, we investigated the an- ti-hyperglycemic effects of MFE in STZ-induced diabetic mice and normal mice after the consumption of starch to- gether with MFE. Postprandial blood glucose levels were significantly decreased in STZ-induced diabetic mice.

대상 데이터

Four-week-old, male ICR mice (Orient Bio Inc., Seong- nam, Korea) were used as test animals and were housed individually in a light (12 hr on/12 hr off) and temper- ature-controlled room with ad libitum access to pelleted food and water. After a 2-week adjustment period, diabetes was induced as described below.

데이터처리

, Cary, NC, USA). Differences among groups were eval- uated by one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test. Differences between two groups were compared using Student’s t-test.
Differences among groups were eval- uated by one-way analysis of variance (ANOVA) followed by Duncan’s multiple range test. Differences between two groups were compared using Student’s t-test. Values of p less than 0.
Each value represents the mean ± SD of seven mice (n = 21). ^a–cValues denoted by different letters are significantly different at p<0.05 as analyzed by Duncan’s multiple range test.
Each value represents the mean ± SD of triplicate experiments. ^a–eValues denoted by different letters are significantly different at p<0.05 as analyzed by Duncan’s multiple range test. Acarbose was used as positive control.

이론/모형

Blood samples were taken from the tail vein at 0, 30, 60, and 120 min after oral administration. Blood glucose level was measured using a glucometer (Roche Diagnostics GmbH) and the area under the glucose response curve (AUC) was calculated using the trapezoidal rule [13].

성능/효과

In conclusion, MFE inhibited α-glucosidase and α-amylase activities and attenuated the rise in blood glucose levels, leading to a reduction in postprandial hyperglycemia. Moreover, MFE may delay the absorption of dietary carbo- hydrates in the gut, hence suppressing the increase in blood glucose levels after a meal.
The inhibitory activity of MFE at varying concentrations was expressed as 100 minus the ab- sorbance change of test compounds relative to the absorb- ance change of the control (%), where the test solution was replaced by the carrier solvent. The measurements were per- formed in triplicate and the IC₅₀ value (the concentration of MFE that results in 50% inhibition of maximal activity) was determined.
[28] reported that dietary anthocyanins in berries significantly reduced post- prandial glucose levels. Therefore, the results of our study suggest that anthocyanin contained in MFE may delay the absorption of dietary starch, thus attenuating the increase in postprandial blood glucose levels. This alleviating effect on postprandial hyperglycemia may be due to the inhibition of carbohydrate digestive enzymes in the small intestine of diabetic mice.

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