목적 : 이 연구는 흰쥐 염증성 통증 모델에서 족궐음간경의 형혈 전침의 진통효과를 조사하고 이 전침의 진통효과에 내인성오피오이드가 관계되어 있는지 조사하고자 하였다. 방법 : 무릎 관절염은 $125{\mu}l$ complete Freund's adjuvant를 흰쥐의 한쪽 무릎관절강에 주입하여 유도하였다. 통증 정도를 검사하기 위해 관절염을 유도한 쪽 뒷다리의 weight bearing forces(WBFs)를 측정하였다. 전침은 건측 행간, 합곡, 또는 뒷다리의 비경혈에 가스 마취하에서 2 Hz, 2 mA 자극조건으로 처치하였다. 결과 : 관절염을 유도한 이후에, 점차적으로 무릎에 통증이 증가하기 때문에 관절염을 유발한 쪽 뒷발로 딛는 압력이 감소하였다. 건측 행간에 전침 자극을 한 군에서 전침 자극 후 최소 2시간까지 유의하게 WBF가 증가하였으며, 이 효과는 구강으로 투여한 indomethacin 5 mg/kg의 효과에 해당하였으며, 이 전침의 효과는 합곡이나 비경혈에서는 나타나지 않았다. 아울러 이 진통효과는 오피오이드 길항제인naltrexone(10 mg/kg, i.p.)을 전처치했을 때 차단되었다. 결론 : 이상의 결과는 족궐음간경의 형혈인 행간 자침이 흰쥐 관절염 모델에서 염증성 통증을 억제하는 효과가 있음을 보여주며, 이 효과는 내인성 오피오이드 시스템이 매개하는 것으로 보인다.
목적 : 이 연구는 흰쥐 염증성 통증 모델에서 족궐음간경의 형혈 전침의 진통효과를 조사하고 이 전침의 진통효과에 내인성 오피오이드가 관계되어 있는지 조사하고자 하였다. 방법 : 무릎 관절염은 $125{\mu}l$ complete Freund's adjuvant를 흰쥐의 한쪽 무릎관절강에 주입하여 유도하였다. 통증 정도를 검사하기 위해 관절염을 유도한 쪽 뒷다리의 weight bearing forces(WBFs)를 측정하였다. 전침은 건측 행간, 합곡, 또는 뒷다리의 비경혈에 가스 마취하에서 2 Hz, 2 mA 자극조건으로 처치하였다. 결과 : 관절염을 유도한 이후에, 점차적으로 무릎에 통증이 증가하기 때문에 관절염을 유발한 쪽 뒷발로 딛는 압력이 감소하였다. 건측 행간에 전침 자극을 한 군에서 전침 자극 후 최소 2시간까지 유의하게 WBF가 증가하였으며, 이 효과는 구강으로 투여한 indomethacin 5 mg/kg의 효과에 해당하였으며, 이 전침의 효과는 합곡이나 비경혈에서는 나타나지 않았다. 아울러 이 진통효과는 오피오이드 길항제인naltrexone(10 mg/kg, i.p.)을 전처치했을 때 차단되었다. 결론 : 이상의 결과는 족궐음간경의 형혈인 행간 자침이 흰쥐 관절염 모델에서 염증성 통증을 억제하는 효과가 있음을 보여주며, 이 효과는 내인성 오피오이드 시스템이 매개하는 것으로 보인다.
Objectives : The present study aimed to examine the analgesic effect of electroacupuncture(EA) applied to the brook point of the Liver meridian in the rat model of inflammatory pain and to investigate involvement of endogenouse opioid in the EA-induced analgesia. Methods : Knee arthritis was induced...
Objectives : The present study aimed to examine the analgesic effect of electroacupuncture(EA) applied to the brook point of the Liver meridian in the rat model of inflammatory pain and to investigate involvement of endogenouse opioid in the EA-induced analgesia. Methods : Knee arthritis was induced by injection of $125{\mu}l$ of complete Freund's adjuvant into the knee joint cavity unilaterally. To examine the level of pain, weight bearing forces(WBFs) of affected limb was measured. EA treatment was given at the LR2, LI4 or non-acupoint on the contralateral limb with frequency of 2 Hz and intensity of 2 mA under gaseous anesthesia. Results : After induction of arthritis, rats subsequently showed a reduced stepping force of the affected hindlimb due to a painful knee. EA applied to the LR2 point on the contralateral hind limb produced a significant improvement of stepping force of the hind limb lasting for at least 2 h, and this effect was equivalent to that obtained by 5 mg/kg of oral indomethacin. The effect of EA was specific to the acupoint since it could not be mimicked by EA applied to the LI4 point or the arbitrary non-acupoint. The analgesic effect was blocked by pretreatment with naltrexone(10 mg/kg, i.p.). Conclusions : These results suggest that acupuncture applied to LR2, brook point of Liver meridian suppresses inflammatory pain in a rat model of knee arthritis and this effect seems to be mediated by endogenous opioid systems.
Objectives : The present study aimed to examine the analgesic effect of electroacupuncture(EA) applied to the brook point of the Liver meridian in the rat model of inflammatory pain and to investigate involvement of endogenouse opioid in the EA-induced analgesia. Methods : Knee arthritis was induced by injection of $125{\mu}l$ of complete Freund's adjuvant into the knee joint cavity unilaterally. To examine the level of pain, weight bearing forces(WBFs) of affected limb was measured. EA treatment was given at the LR2, LI4 or non-acupoint on the contralateral limb with frequency of 2 Hz and intensity of 2 mA under gaseous anesthesia. Results : After induction of arthritis, rats subsequently showed a reduced stepping force of the affected hindlimb due to a painful knee. EA applied to the LR2 point on the contralateral hind limb produced a significant improvement of stepping force of the hind limb lasting for at least 2 h, and this effect was equivalent to that obtained by 5 mg/kg of oral indomethacin. The effect of EA was specific to the acupoint since it could not be mimicked by EA applied to the LI4 point or the arbitrary non-acupoint. The analgesic effect was blocked by pretreatment with naltrexone(10 mg/kg, i.p.). Conclusions : These results suggest that acupuncture applied to LR2, brook point of Liver meridian suppresses inflammatory pain in a rat model of knee arthritis and this effect seems to be mediated by endogenous opioid systems.
* AI 자동 식별 결과로 적합하지 않은 문장이 있을 수 있으니, 이용에 유의하시기 바랍니다.
제안 방법
We reasoned that the reduced weight bearing on the foot after the induction of knee arthritis was because rats were trying to avoid bearing weight on the affected foot due to pain associated with the knee arthritis. To evaluate the validity of using the reduction of foot stepping force as an index for arthritic pain, the effect of oral indomethacin on the stepping force was examined. As shown in Fig.
데이터처리
Asterisks indicate values significantly different from the preoperative value(Pre)(p＜0.05, one-way repeated measures ANOVA followed by Dunnett’s post-hoc test).
Statistical analyses were conducted by the one-way repeated measures analysis of Varience(ANOVA) followed by the Dunnett’s post-hoc test, or two-way repeated measures ANOVA followed by the Duncan’s all pairwise multiple comparison.
Symbols*and＋ denote values significantly different from the pre-injection value (−1 h) and from the control(DW) group, respectively(p＜0.05, two-way repeated measures ANOVA followed by Duncan’s multiple comparison).
Symbols*and＋denote values significantly different from the pre-stimulus value(−1 h) and from the control (Anes Only) group, respectively(p＜0.05, two-way repeated measures ANOVA followed by Duncan’s multiple comparison).
Symbols*and＋denote values significantly different from the pre-treatment value(−1 h) and from NTX＋EA group, respectively(p＜0.05, two-way repeated measures ANOVA followed by Duncan’s multiple comparison).
참고문헌 (26)
Deadman P, Al-Khafaji M, Baker K. A Manual of Acupuncture. Hove: Journal of Chinese Medicine Publications. 2007.
Burckhardt CS. The use of the McGill Pain Questionnaire in assessing arthritis pain. Pain. 1984 ; 19 : 305-14.
Meridians and Acupoints Compilation Committee of Korean Medical Colleges. Principles of Meridians & Acupoints; A Guidebook for College Students. Daejeon: JongRyeoNaMu Publishing Co. 2015.
Colpaert FC. Evidence that adjuvant arthritis in the rat is associated with chronic pain. Pain. 1987 ; 28 : 201-22.
Struthers GR, Scott DL, Scott DG. The use of 'alternative treatments' by patients with rheumatoid arthritis. Rheumatology international. 1983 ; 3 : 151-2.
Stux G, Pomeranz B. Acupuncture Textbook and Atlas. Berlin Heidelberg New York Tokyo: Springer-Verlag. 1987.
Qiao LN, Liu JL, Tan LH, Yang HL, Zhai X, Yang YS. Effect of electroacupuncture on thermal pain threshold and expression of calcitonin-gene related peptide, substance P and gamma-aminobutyric acid in the cervical dorsal root ganglion of rats with incisional neck pain. Acupunct Med. 2017 ; 35 : 276-83.
Chen XH, Han JS. Analgesia induced by electroacupuncture of different frequencies is mediated by different types of opioid receptors: another cross-tolerance study. Behav Brain Res. 1992 ; 47 : 143-9.
Das S, Chatterjee TK, Ganguly A, Ghosh JJ. Role of adrenal steroids on electroacupuncture analgesia and on antagonising potency of naloxone. Pain. 1984 ; 18 : 135-43.
Arichi S, Arichi H, Toda S. Acupuncture and rehabilitation(III) effects of acupuncture applied to the normal side on osteoarthritis deformans and rheumatoid arthritis of the knee and on disorders in motility of the knee joint after cerebral hemorrhage and thrombosis. Am J Chin Med. 1983 ; 11 : 146-9.
Cheng RS, Pomeranz BH. Electroacupuncture analgesia is mediated by stereospecific opiate receptors and is reversed by antagonists of type I receptors. Life Sci. 1980 ; 26 : 631-8.
Min SS, Han JS, Kim YI, Na HS, Yoon YW, Hong SK et al. A novel method for convenient assessment of arthritic pain in voluntarily walking rats. Neurosci Lett. 2001 ; 308 : 95-8.
Schott E, Berge OG, Angeby-Moller K, Hammarstrom G, Dalsgaard CJ, Brodin E. Weight bearing as an objective measure of arthritic pain in the rat. J Pharmacol Toxicol Methods. 1994 ; 31 : 79-83.
※ AI-Helper는 부적절한 답변을 할 수 있습니다.