본 연구에서는 경상북도 울릉군에서 분리한 토양 방선균에 대해 생리학적 특징과 다양성에 대해 연구하였다. ULS1 및 ULS2로 명명한 2개의 토양 시료를 채취하여 다양한 배지에 배양하여 분리하였으며, 평균 생균수는 ULS1 토양은 $1.28{\times}10^7CFU/g$, ULS2 토양은 $2.05{\times}10^7CFU/g$였다. 16S rRNA 유전자에 기반한 염기서열 분석 결과, 총 9개의 속에서 34개의 균주가 분리되었으며 해당 속은 Streptomyces (16 균주), Isoptericola (5 균주), Rhodococcus (4 균주), Agromyces (3 균주), Micrococcus (2 균주), Arthrobacter (1 균주), Williamsia (1 균주), Microbacterium (1 균주) 및 Oerskovia (1 균주)에 속하는 것을 알 수 있었다. 다양한 효소활성과 식물 생장 촉진 활성 측정 결과, 전체의 58.8%가 단백질 분해 활성을, 79.4%가 Tween 80 분해 활성을, 그리고 61.8%가 DNA 분해 활성을 각각 가지는 것으로 나타났다. Oerskovia, Williamsia, Isoptericola 및 Streptomyces 속에 속하는 분리주들로부터 인을 가용화시키는 능력을 확인할 수 있었으며, Agromyces, Oerskovia, Micrococcus, Rhodococcus, Streptomyces 및 Isoptericola 속에 속하는 분리주들은 식물호르몬인 3-indole-acetic acid (IAA)를 생산하는 것을 확인할 수 있었다. Streptomyces 속에 속하는 분리주들은 Candida albicans 뿐만 아니라 Staphylococcus aureus와 Bacillus subtilis에 항생활성을 나타내었다. 본 연구는 독특한 생태계를 구성하는 울릉도 지역의 토양 방선균 다양성 및 생리 활성에 대한 최초의 연구로서 의미를 가지며, 새로운 유용 생리 활성 물질의 좋은 원천이 될 것이라 사료된다.
본 연구에서는 경상북도 울릉군에서 분리한 토양 방선균에 대해 생리학적 특징과 다양성에 대해 연구하였다. ULS1 및 ULS2로 명명한 2개의 토양 시료를 채취하여 다양한 배지에 배양하여 분리하였으며, 평균 생균수는 ULS1 토양은 $1.28{\times}10^7CFU/g$, ULS2 토양은 $2.05{\times}10^7CFU/g$였다. 16S rRNA 유전자에 기반한 염기서열 분석 결과, 총 9개의 속에서 34개의 균주가 분리되었으며 해당 속은 Streptomyces (16 균주), Isoptericola (5 균주), Rhodococcus (4 균주), Agromyces (3 균주), Micrococcus (2 균주), Arthrobacter (1 균주), Williamsia (1 균주), Microbacterium (1 균주) 및 Oerskovia (1 균주)에 속하는 것을 알 수 있었다. 다양한 효소활성과 식물 생장 촉진 활성 측정 결과, 전체의 58.8%가 단백질 분해 활성을, 79.4%가 Tween 80 분해 활성을, 그리고 61.8%가 DNA 분해 활성을 각각 가지는 것으로 나타났다. Oerskovia, Williamsia, Isoptericola 및 Streptomyces 속에 속하는 분리주들로부터 인을 가용화시키는 능력을 확인할 수 있었으며, Agromyces, Oerskovia, Micrococcus, Rhodococcus, Streptomyces 및 Isoptericola 속에 속하는 분리주들은 식물호르몬인 3-indole-acetic acid (IAA)를 생산하는 것을 확인할 수 있었다. Streptomyces 속에 속하는 분리주들은 Candida albicans 뿐만 아니라 Staphylococcus aureus와 Bacillus subtilis에 항생활성을 나타내었다. 본 연구는 독특한 생태계를 구성하는 울릉도 지역의 토양 방선균 다양성 및 생리 활성에 대한 최초의 연구로서 의미를 가지며, 새로운 유용 생리 활성 물질의 좋은 원천이 될 것이라 사료된다.
Actinobacteria tolerating extreme conditions can be a rich source of bioactive compounds and enzymes. In this study filamentous actinobacteria were isolated from soils of Ulleung Island, and their physiological properties were examined. Soil samples were collected, serially diluted and spread on var...
Actinobacteria tolerating extreme conditions can be a rich source of bioactive compounds and enzymes. In this study filamentous actinobacteria were isolated from soils of Ulleung Island, and their physiological properties were examined. Soil samples were collected, serially diluted and spread on various agar media. The average viable counts of total bacteria were $1.28{\times}10^7CFU/g$ for soil sample 1 (ULS1) and $2.05{\times}10^7CFU/g$ for soil sample 2 (ULS2). As a result, 34 strains of actinobacteria were isolated and assigned to the genera Streptomyces (16 strains), Isoptericola (5 strains), Rhodococcus (4 strains), Agromyces (3 strains), Micrococcus (2 strains), Arthrobacter (1 strain), Williamsia (1 strain), Microbacterium (1 strain), and Oerskovia (1 strain) based on 16S rRNA gene sequence analysis. Enzyme activity and plant growth promoting potential were tested for representative isolates. Multiple strains of Streptomyces degraded starch, casein and Tween 80. As for plant growth promoting potential, strains of Oerskovia, Williamsia, Isoptericola, and Streptomyces solubilized phosphate, and those of Agromyces, Oerskovia, Micrococcus, Rhodococcus, Streptomyces, and Isoptericola produced 3-indole-acetic acid (IAA), respectively. Selected strains of Streptomyces exhibited strong antagonistic activity against Staphylococcus aureus and Bacillus subtilis as well as Candida albicans. This study confirms that actinobacteria from Ulleung Island can be a good source of novel bioactive compounds.
Actinobacteria tolerating extreme conditions can be a rich source of bioactive compounds and enzymes. In this study filamentous actinobacteria were isolated from soils of Ulleung Island, and their physiological properties were examined. Soil samples were collected, serially diluted and spread on various agar media. The average viable counts of total bacteria were $1.28{\times}10^7CFU/g$ for soil sample 1 (ULS1) and $2.05{\times}10^7CFU/g$ for soil sample 2 (ULS2). As a result, 34 strains of actinobacteria were isolated and assigned to the genera Streptomyces (16 strains), Isoptericola (5 strains), Rhodococcus (4 strains), Agromyces (3 strains), Micrococcus (2 strains), Arthrobacter (1 strain), Williamsia (1 strain), Microbacterium (1 strain), and Oerskovia (1 strain) based on 16S rRNA gene sequence analysis. Enzyme activity and plant growth promoting potential were tested for representative isolates. Multiple strains of Streptomyces degraded starch, casein and Tween 80. As for plant growth promoting potential, strains of Oerskovia, Williamsia, Isoptericola, and Streptomyces solubilized phosphate, and those of Agromyces, Oerskovia, Micrococcus, Rhodococcus, Streptomyces, and Isoptericola produced 3-indole-acetic acid (IAA), respectively. Selected strains of Streptomyces exhibited strong antagonistic activity against Staphylococcus aureus and Bacillus subtilis as well as Candida albicans. This study confirms that actinobacteria from Ulleung Island can be a good source of novel bioactive compounds.
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문제 정의
This study is the first report on the culturable diversity of actinobacteria in Ulleung Island soils. High level of taxonomic diversity of actinobacteria, and also high activities of hydrolytic enzymes were confirmed through this study.
Streptomyces 속에 속하는 분리주들은 Candida albicans 뿐만 아니라 Staphylococcus aureus와 Bacillus subtilis에 항생활성을 나타내었다. 본 연구는 독특한 생태계를 구성하는 울릉도 지역의 토양 방선균 다양성 및 생리 활성에 대한 최초의 연구로서 의미를 가지며, 새로운 유용 생리 활성 물질의 좋은 원천이 될 것이라 사료된다.
본 연구에서는 경상북도 울릉군에서 분리한 토양 방선균에 대해 생리학적 특징과 다양성에 대해 연구하였다. ULS1 및 ULS2로 명명한 2개의 토양 시료를 채취하여 다양한 배지에 배양하여 분리하였으며, 평균 생균수는 ULS1 토양은 1.
제안 방법
The island is thus expected to have unique microbial communities, but little is known on the microbial diversity, in particular in soil environment. In this study, taxonomic diversity and physiological properties of filamentous actinobacteria isolated from Ulleung Island soils were examined, and the antimicrobial activities and hydrolysis of macromolecules were evaluated for the isolates.
ULS1 및 ULS2로 명명한 2개의 토양 시료를 채취하여 다양한 배지에 배양하여 분리하였으며, 평균 생균수는 ULS1 토양은 1.28 × 107 CFU/g, ULS2 토양은 2.05 × 107 CFU/g였다.
대상 데이터
Test organisms obtained from the Korea Collection for type Cultures (KCTC) and the Culture Collection of Antimicrobial Resistant Microbes (CCARM) were used. The isolates were challenged against three Gram-negative bacteria Klebsiella pneumoniae KCTC 2208, Salmonella enterica KCTC 12456, and Pseudomonas aeruginosa CCARM 0222, three Gram-positive bacteria, Bacillus subtilis CCARM 0003, Micrococcus luteus CCARM 0022, and Staphylococcus aureus CCARM 0022, and two yeasts, Candida albicans KCTC 7270 and Candida krusei CCARM 14017. On tryptic soy agar plates with lawns of test microbes, individual isolates were spotted and incubated for 7 days at 30°C.
Two soil samples were collected from Ulleung Island, Republic of Korea in February 2016, and designated ULS1 (GPS 37° 29′ 19.7″ N, 130° 54′ 14.7″ E) and ULS2 (GPS 37° 28′ 44.4″ N, 130° 51′ 16.8″ E) (Fig. 1).
이론/모형
The sequences were aligned with those of reference taxa retrieved from public databases. Distance based phylogenetic trees were generated using the distance model of Jukes and Cantor (1969) and neighbor-joining algorithm (Saitou and Nei, 1987). The tree reliability was estimated by bootstrapping with 1,000 replicates.
The purified PCR products were sequenced using the service of Macrogen. The 16S rRNA gene sequences of the isolates were compared with other sequences in the EzTaxon database (http://eztaxon-e.ezbiocloud.net; Kim et al., 2012) based on the pairwise alignment method.
Clearing zones were detected after incubation at 30°C for 7 days for all tests. The production of indole-3-acetic acid (IAA), a plant growth hormone, was determined using the Salkowski colorimetric technique developed by Glickmann and Dessaux (1995). Actinobacterial isolates were inoculated to tryptic soy broth containing 0.
성능/효과
05 × 107 CFU/g였다. 16S rRNA 유전자에 기반한 염기서열 분석 결과, 총 9개의 속에서 34개의 균주가 분리되었으며 해당 속은 Streptomyces (16 균주), Isoptericola (5 균주), Rhodococcus (4 균주), Agromyces (3 균주), Micrococcus (2 균주), Arthrobacter (1 균주), Williamsia (1 균주), Microbacterium (1 균주) 및 Oerskovia (1 균주)에 속하는 것을 알 수 있었다. 다양한 효소활성과 식물 생장 촉진 활성 측정 결과, 전체의 58.
2~61 mg/L (Table 1). Among them, Streptomyces was the most dominant producers of IAA (32%), which was then followed by Agromyces (12%), Rhodococcus (12%), Isoptericola (8%), Micrococcus (8%), Microbacterium (4%), Oerskovia (4%), and Williamsia (4%), respectively. Micrococcus sp.
In summary, all actinobacterial isolates were assigned to the genera Streptomyces (16 strains), Isoptericola (5 strains), Rhodococcus (4 strains), Agromyces (3 strains), Micrococcus (2 strains), Arthrobacter (1 strain), Williamsia (1 strain), Microbacterium (1 strain), and Oerskovia (1 strain). Notably, most taxa were non-filamentous, pleomorphic groups except for Streptomyces and Oerskovia (Goodfellow, 2012).
8%가 DNA 분해 활성을 각각 가지는 것으로 나타났다. Oerskovia, Williamsia, Isoptericola 및 Streptomyces 속에 속하는 분리주들로부터 인을 가용화시키는 능력을 확인할 수 있었으며, Agromyces, Oerskovia, Micrococcus, Rhodococcus, Streptomyces 및 Isoptericola 속에 속하는 분리주들은 식물호르몬인 3-indole-acetic acid (IAA)를 생산하는 것을 확인할 수 있었다. Streptomyces 속에 속하는 분리주들은 Candida albicans 뿐만 아니라 Staphylococcus aureus와 Bacillus subtilis에 항생활성을 나타내었다.
Oerskovia, Williamsia, Isoptericola 및 Streptomyces 속에 속하는 분리주들로부터 인을 가용화시키는 능력을 확인할 수 있었으며, Agromyces, Oerskovia, Micrococcus, Rhodococcus, Streptomyces 및 Isoptericola 속에 속하는 분리주들은 식물호르몬인 3-indole-acetic acid (IAA)를 생산하는 것을 확인할 수 있었다. Streptomyces 속에 속하는 분리주들은 Candida albicans 뿐만 아니라 Staphylococcus aureus와 Bacillus subtilis에 항생활성을 나타내었다. 본 연구는 독특한 생태계를 구성하는 울릉도 지역의 토양 방선균 다양성 및 생리 활성에 대한 최초의 연구로서 의미를 가지며, 새로운 유용 생리 활성 물질의 좋은 원천이 될 것이라 사료된다.
16S rRNA 유전자에 기반한 염기서열 분석 결과, 총 9개의 속에서 34개의 균주가 분리되었으며 해당 속은 Streptomyces (16 균주), Isoptericola (5 균주), Rhodococcus (4 균주), Agromyces (3 균주), Micrococcus (2 균주), Arthrobacter (1 균주), Williamsia (1 균주), Microbacterium (1 균주) 및 Oerskovia (1 균주)에 속하는 것을 알 수 있었다. 다양한 효소활성과 식물 생장 촉진 활성 측정 결과, 전체의 58.8%가 단백질 분해 활성을, 79.4%가 Tween 80 분해 활성을, 그리고 61.8%가 DNA 분해 활성을 각각 가지는 것으로 나타났다. Oerskovia, Williamsia, Isoptericola 및 Streptomyces 속에 속하는 분리주들로부터 인을 가용화시키는 능력을 확인할 수 있었으며, Agromyces, Oerskovia, Micrococcus, Rhodococcus, Streptomyces 및 Isoptericola 속에 속하는 분리주들은 식물호르몬인 3-indole-acetic acid (IAA)를 생산하는 것을 확인할 수 있었다.
후속연구
Strains of Oerskovia and Agromyces were antagonistic against fungi or bacteria, although strains of Streptomyces formed the majority of isolates with antimicrobial activities. More detailed investigation is required to demonstrate the potential of these organisms for the biocontrol of pathogenic fungi or bacteria.
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