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NTIS 바로가기생명과학회지 = Journal of life science, v.27 no.1 = no.201, 2017년, pp.50 - 56
김태영 (부경대학교 수산과학대학 생물공학과) , 고혜진 (부경대학교 수산과학대학 생물공학과) , 박남규 (부경대학교 수산과학대학 생물공학과)
An antibacterial peptide was purified from an acidified gill extract of the pufferfish Takifugu pardalis. The acidified gill extract was put through a Sep-Pak C18 solid phase extraction cartridge using a stepwise gradient and divided into a flow-through (F.T.) and 60% methanol fraction (RM 60). Amon...
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핵심어 | 질문 | 논문에서 추출한 답변 |
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Histone은 무엇인가? | Histone은 염색질을 구성하는 중심 단백질이며, H1, H2, H2A, H2B, H3 및 H4와 같이 6종류의 histone이 알려져 있다. 이들은 DNA의 응축을 도우며, 유전자 발현조절에 중요한 역할을 한다. | |
PFG의 분자량은 얼마인가? | 정제한 펩타이드의 분자량 및 아미노산 서열을 알아보기 위해 MALDI-TOF Mass와 아미노산서열 분석기로 측정하였다. PFG의 분자량은 1171.595 Da이었고(Fig. 4B), 이 물질의 일차구조는 10개의 아미노산 잔기로 구성된 STKEKAPRKQOH로 판명되었다. | |
향균활성 펩타이드 정제 물질에 단백질 분해효소 trypsin 처리 시, PFG의 활성은 어떻게 나타나는가? | 정제한 물질의 화학적 특성인 안정성 효과를 알아보기 위해서 pH, 온도 및 효소처리를 하여 항균활성을 측정하였다(Table 1). PFG는 단백질 분해효소인 trypsin을 처리하기 전에는 항균활성을 나타내었지만(Table 1A), trypsin을 처리한 후에는 대부분 활성이 소실되었다(Table 1B). 그러나 열(100℃)을 가하거나 산성 및 염기성 조건하에서는 활성이 그대로 유지되었다(Table 1C - Table 1E). |
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