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제안 방법
- Among different barcoding methods, those targeting the ITS region have the greatest probability of successful identification of the broadest range of fungi[20, 26-28]. In this study, molecular identification of 11 Trichoderma isolates was conducted using the ITS sequence (Table 1). The results revealed that the Korea Agricultural Culture Collection (KACC) strains 40552 and 40557 shared 99% sequence identity with each other and were 100% homologous with both T.
- Five Trichoderma isolates (KACC 40552, 40557, 40929, 40931, and 41717) showed strong inhibitory activity against seven Phytophthora species. In this study, we further characterized the selected isolates through analyses of the ITS region, metabolic profiling, and CWDE activities.
- In this study, we identified and characterized selected Trichoderma isolates by metabolic profiling and enzyme assay to evaluate their potential for use as biocontrol agents against plant pathogens.
- The cell walls of most fungi are chitin-based, and disruption of the cell walls has profound effects on cell growth and morphology. In this study, we measured the activities of various CWDEs from five Trichoderma isolates (KACC 40552, 40557, 40929, 40931, and 41717) to determine which isolates had the potential for use as biocontrol agents with the ability to degrade cell walls of fungal pathogens (Table 3). High enzyme activities for Avicel, CMC, xylan, β-glucan, pectin, and chitin degradation were found in KACC 41717, 40929, 41717, 40552/40557, 41717, and 41717, respectively.
- Metabolic profiling of the EtOAc extracts of five Trichoderma isolates (KACC 40552, 40557, 40929, 40931, and 41717) was conducted using an Agilent 6890N GC (Agilent, USA) fitted with a HP-5MS capillary column (30 m × 0.25 mm × 0.25 μm) linked to a JMS 700 mass spectrometer (Jeol, Japan) at the Korea Basic Science Institute (Korea).
대상 데이터
- All Trichoderma isolates were obtained from the Rural Development Administration (RDA) Genebank Information Center (GIC) (Wanju, Republic of Korea). Isolates were maintained on potato dextrose agar (PDA) (Difco, USA) at 25℃ for 14 days under dark conditions.
- atroviride. The results of the present study matched the identification determined by the RDA GIC for KACC 40776 (T. atroviride), 40871 (T. harzianum), and 40929 (T. virens). Three isolates (KACC 40931, 41707, and 41717) were homologous with T.
데이터처리
- Duncan’s multiple-range test was applied to identify differences among groups at a significance of p ≤ 0.05 using the SAS software (SAS Inc., USA).
이론/모형
- Cell-free supernatants were then harvested by centrifugation at 6,300 ×g for 20 min at 4℃, after which the protein concentrations of the Trichoderma liquid cultures were measured by the Bradford method.
- Phylogenetic tree based on internal transcribed spacer (ITS) sequences of the selected Trichoderma isolates. The tree was generated from the ITS sequences of Trichoderma isolates by the neighbor-joining method using MEGA5 (http://www/ megasoftware.net/). The numbers at the nodes indicate bootstrap values from 1,000 replications.
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