Chitosan was prepared by deacetylation of chitin composed of an N-acetyl-D-glucosamine unit. In general, a fluorescent material contains atomic groups that exhibit a luminescent effect, and is known to fluoresce, owing to the excitation and emission processes at a low energy state. Organic compounds exhibiting such fluorescence can be applied in bio-imaging and detection. In a previous study, chitosan oligomers were reported to exhibit fluorescence in an aqueous solution, owing to their unique molecular structure properties. In this study, the effect of solution parameters on the fluorescence properties of chitosan were investigated in terms of the polymer parameters (molecular weight, degree of deacetylation, and concentration) and solvent parameter (concentration of acetic acid). The fluorescent intensity of chitosan increased at a lower molecular weight, higher degree of deacetylation, and lower acetic acid concentration.
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