남한산 큰자색호랑꽃무지는 그 동안 일본에 분포하는 Osmoderma opicum으로 동정되어 왔으며, 국내에서는 그 희귀성에 의해 멸종위기종으로 다루어지고 있지만, 남한 개체군에 대한 면밀한 종의 실체에 대한 구명은 없었다. 이에 대해, 남한산 큰자색호랑꽃무지와 유라시안 근연종에 대해 형태 및 COI유전자 염기서열을 비교를 실시하였다. 결과적으로, 남한산 개체군은 형태적으로 극동러시아에 분포하는 O. caeleste와 동일하였다. 따라서, 남한산 큰자색호랑꽃무지는 O. caeleste임을 제안한다.
남한산 큰자색호랑꽃무지는 그 동안 일본에 분포하는 Osmoderma opicum으로 동정되어 왔으며, 국내에서는 그 희귀성에 의해 멸종위기종으로 다루어지고 있지만, 남한 개체군에 대한 면밀한 종의 실체에 대한 구명은 없었다. 이에 대해, 남한산 큰자색호랑꽃무지와 유라시안 근연종에 대해 형태 및 COI유전자 염기서열을 비교를 실시하였다. 결과적으로, 남한산 개체군은 형태적으로 극동러시아에 분포하는 O. caeleste와 동일하였다. 따라서, 남한산 큰자색호랑꽃무지는 O. caeleste임을 제안한다.
The South Korean hermit beetle has previously been identified as Osmoderma opicum, which is distributed in Japan. Because of its rarity, this species is classified as an endangered species. To date, however, the identity of this species in South Korea has not been conclusively confirmed. To assess t...
The South Korean hermit beetle has previously been identified as Osmoderma opicum, which is distributed in Japan. Because of its rarity, this species is classified as an endangered species. To date, however, the identity of this species in South Korea has not been conclusively confirmed. To assess the taxonomic status of the hermit beetle occurring in South Korea, we performed a comparative study with the beetle's Eurasian congeners, based on morphological examination and molecular analysis using COI gene sequences. The results clearly showed that the South Korean hermit beetle is identical to Osmoderma caeleste, which has been described from the Russian Far East. Therefore, we suggest that the taxonomic identity of the South Korean hermit beetle is O. caeleste.
The South Korean hermit beetle has previously been identified as Osmoderma opicum, which is distributed in Japan. Because of its rarity, this species is classified as an endangered species. To date, however, the identity of this species in South Korea has not been conclusively confirmed. To assess the taxonomic status of the hermit beetle occurring in South Korea, we performed a comparative study with the beetle's Eurasian congeners, based on morphological examination and molecular analysis using COI gene sequences. The results clearly showed that the South Korean hermit beetle is identical to Osmoderma caeleste, which has been described from the Russian Far East. Therefore, we suggest that the taxonomic identity of the South Korean hermit beetle is O. caeleste.
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제안 방법
, 2012) (Table 2). PCR was conducted using AccuPower ProFi Taq PCR PreMix (Bioneer, Daejeon, Korea). The amplification profile consisted of pre-denaturation for 5 min at 95℃, 35-45 cycles of 30 s at 95℃ for denaturation, 30 s at 48-53℃ for primer annealing, and then 45 s at 72℃ for extension, with a final extension for 5 min at 72℃.
대상 데이터
Sequences were finally trimmed to 657-bp fragments. COI sequences generated in this study are available from GenBank under the accession numbers KX346561-76 (Table 1). For molecular species identification, a neighbor-joining (NJ) analysis (Saitou and Nei 1987) was performed using MEGA 5.
이론/모형
Genetic distances within and between species of hermit beetle were calculated using Kimura’s two-parameter test (Kimura, 1980) in accordance with Nei’s empirical guidelines (Nei 1991, 1996).
성능/효과
A total of 16 COI sequences representing three morphospeices, O. opicum, O. barnabita, and O. caeleste including the Korean population, were successfully generated. Before analyzing the COI sequences, we confirmed the absence of
후속연구
Among them, the distributions of China, Mongolia and Korea were listed having no the basis of examined specimen records. Therefore, it is needed that the exact distributional range of this species has to reexamine in the further study.
참고문헌 (36)
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