Seven unrecorded species in the phylum Ascomycota, Emericellopsis pallida (KNU16-167), Scedosporium aurantiacum (KNU16-190), Duddingtonia flagrans (KNU16-279), Bionectria rossmaniae (KNU16-309), Exophiala xenobiotica (KNU16-79), Pseudocercosporella fraxini (KNU16-102), and Stachybotrys sansevieriae ...
Seven unrecorded species in the phylum Ascomycota, Emericellopsis pallida (KNU16-167), Scedosporium aurantiacum (KNU16-190), Duddingtonia flagrans (KNU16-279), Bionectria rossmaniae (KNU16-309), Exophiala xenobiotica (KNU16-79), Pseudocercosporella fraxini (KNU16-102), and Stachybotrys sansevieriae (KNU16-141), were isolated in 2016 from field soils collected from various locations in Korea. All of the species were identified and described based on morphological characteristics and rDNA internal transcribed spacer sequence data. Morphological features of these fungi were examined on potato dextrose agar, oatmeal agar, malt extract agar, Czapek yeast extract agar, and yeast extract sucrose agar. Full descriptions and illustrations of their morphological characteristics are provided.
Seven unrecorded species in the phylum Ascomycota, Emericellopsis pallida (KNU16-167), Scedosporium aurantiacum (KNU16-190), Duddingtonia flagrans (KNU16-279), Bionectria rossmaniae (KNU16-309), Exophiala xenobiotica (KNU16-79), Pseudocercosporella fraxini (KNU16-102), and Stachybotrys sansevieriae (KNU16-141), were isolated in 2016 from field soils collected from various locations in Korea. All of the species were identified and described based on morphological characteristics and rDNA internal transcribed spacer sequence data. Morphological features of these fungi were examined on potato dextrose agar, oatmeal agar, malt extract agar, Czapek yeast extract agar, and yeast extract sucrose agar. Full descriptions and illustrations of their morphological characteristics are provided.
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문제 정의
The purpose of this study was the morphological and molecular identification of these seven fungi that were newly discovered in Korea.
This research was funded by a grant from the National Institute of Biological Resources (NIBR), the Ministry of Environment of the Republic of Korea to survey and discover indigenous fungal species in Korea. This research was also sponsored by a Research Grant of the Industry-University Cooperation Foundation of Kangwon National University, Chuncheon, Korea.
제안 방법
For molecular phylogenetic identification, the isolates were grown on PDA for 1 week, and total genomic DNA was extracted using a DNeasy Plant mini kit (Qiagen, Germantown, MD, USA) following the manufacturer’s instructions.
대상 데이터
For the micro-morphological examination, microscopic mounts of all isolates were prepared in lactic acid from colonies grown on PDA, and a drop of alcohol was added to remove air bubbles and excess conidia. Photomicrographs of the isolates were taken with an HK 3.1 CMOS digital camera (KOPTIC, Seoul, Korea) attached to an Olympus BX50F-3 microscope (Olympus). The microscopic structures of the isolates were also examined under a scanning electron microscope (LEO Model 1450VP; Carl Zeiss, Oberkochen, Germany).
The statistical confidence of the tree topology was evaluated based on a bootstrap analysis of 1,000 replicates. The sequences of all of the newly discovered fungi were deposited in the National Institute of Biological Resources (NIBR), Korea, and in GenBank (Table 1).
The isolate KNU16-167 formed a monophyletic group with two reference sequences of Eme. pallida (CBS 624.73) and KU933708 (Fig. 8). Specimen KNU16-309 grouped with two reference sequences of B.
데이터처리
A phylogenetic tree was constructed using the neighbor-joining method in MEGA6 [12]. The statistical confidence of the tree topology was evaluated based on a bootstrap analysis of 1,000 replicates. The sequences of all of the newly discovered fungi were deposited in the National Institute of Biological Resources (NIBR), Korea, and in GenBank (Table 1).
이론/모형
gov/blast/]). A phylogenetic tree was constructed using the neighbor-joining method in MEGA6 [12]. The statistical confidence of the tree topology was evaluated based on a bootstrap analysis of 1,000 replicates.
The amplified PCR products were sequenced on an ABI Prism 3730 DNA analyzer (Applied Biosystems, Foster City, CA, USA). All sequence information was analyzed using the BLAST program (National Center for Biotechnology Information [NCBI], Bethesda, USA[http://www.ncbi.nlm.nih.gov/blast/]). A phylogenetic tree was constructed using the neighbor-joining method in MEGA6 [12].
성능/효과
Based on these morphological and molecular characteristics, the studied fungal isolates (Exo. xenobiotica KNU16-79, P. fraxini KNU16-102, Sta. sansevieriae KNU16-141, Emericellopsis pallida KNU16-167, Sce. aurantiacum KNU16-190, D. flagrans KNU16-279, and B. rossmaniae KNU16-309) were identified. This is the first report of these fungi from soil of Korea.
Seven species were identified within seven different genera: Emericellopsis pallida, Scedosporium aurantiacum, Duddingtonia flagrans, Bionectria rossmaniae, Stachybotrys sansevieriae, Exophiala xenobiotica and Pseudocercosporella fraxini. A neighbor-joining tree was constructed by combining all genera with a clear sequence identity.
참고문헌 (12)
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