Expression of collagenases (matrix metalloproteinase-1, 8, 13) and tissue inhibitor of metalloproteinase-1 of retrodiscal tissue in temporomandibular joint disorder patients원문보기
Objectives: The aim of this study was to reveal how collagenases (matrix metalloproteinase [MMP]-1, 8, 13) and tissue inhibitor of metalloproteinase 1 (TIMP-1) are expressed in immunohistochemistry of retrodiscal tissue in temporomandibular joint disorder patients. Materials and Methods: This study ...
Objectives: The aim of this study was to reveal how collagenases (matrix metalloproteinase [MMP]-1, 8, 13) and tissue inhibitor of metalloproteinase 1 (TIMP-1) are expressed in immunohistochemistry of retrodiscal tissue in temporomandibular joint disorder patients. Materials and Methods: This study was conducted on 39 patients who underwent discoplasty or discectomy. Immunohistochemical staining was undertaken and expression levels of MMP-1, 8, 13, and TIMP-1 were evaluated. The status of internal derangement of disc, osteoarthritis, and joint effusion were analyzed using magnetic resonance imaging (MRI). Disc status observed during operation was also categorized. Results: The more severe disc derangement was observed on MRI, the more increased expression of MMPs and TIMP-1 appeared. Regarding MMP-13 expression, 86.7% of late-stage disc displacement patients showed grade II or III. Expression level of MMPs or TIMP was not statistically significant associated with joint effusion level. In perforation and/or adhesion groups, all patients showed grade II or III expression of MMP-13. Once perforation occurred, MMP-13 showed increased expression with statistical significance. Conclusion: MMP-1 and MMP-13 expression seem to be related to progression of osteoarthritis whereas MMP-8 does not seem to have a specific role with regard to temporomandibular joint disorders. TIMP-1 is considered to be partly related to internal derangement rather than osteoarthritis, but it is not significant.
Objectives: The aim of this study was to reveal how collagenases (matrix metalloproteinase [MMP]-1, 8, 13) and tissue inhibitor of metalloproteinase 1 (TIMP-1) are expressed in immunohistochemistry of retrodiscal tissue in temporomandibular joint disorder patients. Materials and Methods: This study was conducted on 39 patients who underwent discoplasty or discectomy. Immunohistochemical staining was undertaken and expression levels of MMP-1, 8, 13, and TIMP-1 were evaluated. The status of internal derangement of disc, osteoarthritis, and joint effusion were analyzed using magnetic resonance imaging (MRI). Disc status observed during operation was also categorized. Results: The more severe disc derangement was observed on MRI, the more increased expression of MMPs and TIMP-1 appeared. Regarding MMP-13 expression, 86.7% of late-stage disc displacement patients showed grade II or III. Expression level of MMPs or TIMP was not statistically significant associated with joint effusion level. In perforation and/or adhesion groups, all patients showed grade II or III expression of MMP-13. Once perforation occurred, MMP-13 showed increased expression with statistical significance. Conclusion: MMP-1 and MMP-13 expression seem to be related to progression of osteoarthritis whereas MMP-8 does not seem to have a specific role with regard to temporomandibular joint disorders. TIMP-1 is considered to be partly related to internal derangement rather than osteoarthritis, but it is not significant.
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문제 정의
Since most human studies have been carried out using synovial fluid, the production site of these enzymes has never been investigated in degenerated disc tissue. The purpose of this study was to identify MMPs and TIMP-1 immunoreactive patterns with retrodiscal tissue samples obtained during surgery. This study also evaluated the association between these enzymes from retrodiscal tissue and the state of TMJ such as ID, OA, adhesion or perforation of disc.
This study was aimed to observe how collagenases (MMP1, 8, 13) and TIMP-1 are expressed in immunohistochemistry of retrodiscal tissue sections in order to understand how their expression pattern is associated with ID, OA, joint effusion, and adhesion or perforation among TMJ disorder patients.
가설 설정
(3) EDDsR: The disc displaced relative to the condylar head, regardless of jaw position. Disc slightly thickens at its posterior edge or begins to be deformed anatomically.
제안 방법
participated in the study design and performed the statistical analysis. K.H.P. and J.K.H. participated in the study design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
MRI findings for ID were classified into four groups—normal position, disc displacement with reduction (DDcR), early stage of disc displacement without reduction (EDDsR), and late stage of disc displacement without reduction (LDDsR), based upon the position and status of joint disc on T-1 weighted MRI9.
But because of difficulties in subject selection, current studies are insufficient to find out extracellular matrix-related factors for these changes. Therefore in this study, MMP-1, 8, 13 and TIMP-1 expression were investigated from retrodiscal tissue sections obtained during TMJ open surgery. To understand the relationship between expression of these enzymes and TMJ structural changes, immunohistochemical findings, magnetic resonance imaging (MRI), and operative findings were analyzed.
This study also evaluated the association between these enzymes from retrodiscal tissue and the state of TMJ such as ID, OA, adhesion or perforation of disc.
Therefore in this study, MMP-1, 8, 13 and TIMP-1 expression were investigated from retrodiscal tissue sections obtained during TMJ open surgery. To understand the relationship between expression of these enzymes and TMJ structural changes, immunohistochemical findings, magnetic resonance imaging (MRI), and operative findings were analyzed.
대상 데이터
This study was conducted on 39 TMJs of 39 patients who had undergone discoplasty or discectomy. Patients were diagnosed as ID or OA based upon clinical examination and MRI at the Department of Oral and Maxillofacial Surgery at Gangnam Severance Hospital (Seoul, Korea).
This study was conducted on 39 TMJs of 39 patients who had undergone discoplasty or discectomy. Patients were diagnosed as ID or OA based upon clinical examination and MRI at the Department of Oral and Maxillofacial Surgery at Gangnam Severance Hospital (Seoul, Korea).
데이터처리
Data analysis was performed by chi-square test and Fisher’s exact test of cross tabulation analysis using IBM SPSS Statistics ver. 12.0 for Windows (SPSS Inc., Chicago, IL, USA).
성능/효과
In conclusion, MMP-13 plays a critical role in degrading of OA as it is the predominant collagenase in collagen type II and can worsen ID as the collage breakdown enzyme of retrodiscal tissue in TMJ. MMP-1 is related to degeneration of OA.
19 reported it has been established that although individual enzymes have similar substrate specificities, their expression pattern is often distinct and characteristic in a certain tissue and cell type. In this study, all cases showed MMP-1, 8, 13 and TIMP-1 expression but the range or cell type of expression were different. MMP-13, so-called ‘osteoblast collagenase’, demonstrated high expression grade generally.
TIMP-1 seems to affect ID in comparison with OA expression, but it is not significant. In this study, the causal relation of the imbalance between MMP and TIMP could not be explained, but TIMP activity, which shows a difference with degeneration of TMJ disorders, suggests that TIMP should be considered in potential criteria for prevention of progressive disease via controlling collagenases.
The expression levels of MMPs and TIMP-1 are shown in Table 1. MMP1 and MMP-8 were expressed in all cases, and 64% of cases showed grade I expression. MMP-13 was expressed in all cases; however, 44% and 41% of cases showed grade II and III expression.
’s study24 demonstrated that structural modifications of articular disc could be specific responses to functional change of TMJ and variations in extrinsic stimuli may activate intrinsic factors, such as MMPs, that induce structural modifications in discal tissue. The examination found that only the areas with myxoid change or hyalinization were stained or these areas resulted in much more staining than others, and that cell numbers increased in areas with myxoid change. Such differences appeared when the retrodiscal tissue deteriorated or responded to passed-on loading caused by articular disc displacement, which suggests an association with MMPs.
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