Shin, Won-Sub
(Department of Chemical and Biomolecular Engineering, KAIST)
,
Jung, Simon MoonGeun
(Korea Research Institute of Chemical Technology)
,
Cho, Chang-Ho
(Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
,
Woo, Do-Wook
(Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
,
Kim, Woong
(Department of Environmental Engineering, Kyungpook National University)
,
Kwon, Jong-Hee
(Department of Food Science and Technology, Institute of Agriculture and Life Science, Gyeongsang National University)
The spectral composition of light can affect the growth and biochemical composition of photosynthetic microalgae. This study examined the use of light filtering through a solution of soluble colored additives, a cost-effective method to alter the light spectrum, on the growth and lipid production of...
The spectral composition of light can affect the growth and biochemical composition of photosynthetic microalgae. This study examined the use of light filtering through a solution of soluble colored additives, a cost-effective method to alter the light spectrum, on the growth and lipid production of an oleaginous microalga, Nannochloropsis gaditana (N. gaditana). Cells were photoautotrophically cultivated under a white light emitting diode (LED) alone (control) or under a white LED that passed through a solution of red and yellow color additive (4:1 ratio) that blocked light below 600 nm. The specific growth rate was significantly greater under filtered light than white light ($0.2672d^{-1}$ vs. $0.1930d^{-1}$). Growth under filtered light also increased the fatty acid methyl ester (FAME) yield by 22.4% and FAME productivity by 80.0%, relative to the white light control. In addition, the content of saturated fatty acids was greater under filtered light, so the biodiesel products had better stability. These results show that passing white light through an inexpensive color filter can simultaneously enhance cellular growth and lipid productivity of N. gaditana. This approach of optimizing the light spectrum may be applicable to other species of microalgae.
The spectral composition of light can affect the growth and biochemical composition of photosynthetic microalgae. This study examined the use of light filtering through a solution of soluble colored additives, a cost-effective method to alter the light spectrum, on the growth and lipid production of an oleaginous microalga, Nannochloropsis gaditana (N. gaditana). Cells were photoautotrophically cultivated under a white light emitting diode (LED) alone (control) or under a white LED that passed through a solution of red and yellow color additive (4:1 ratio) that blocked light below 600 nm. The specific growth rate was significantly greater under filtered light than white light ($0.2672d^{-1}$ vs. $0.1930d^{-1}$). Growth under filtered light also increased the fatty acid methyl ester (FAME) yield by 22.4% and FAME productivity by 80.0%, relative to the white light control. In addition, the content of saturated fatty acids was greater under filtered light, so the biodiesel products had better stability. These results show that passing white light through an inexpensive color filter can simultaneously enhance cellular growth and lipid productivity of N. gaditana. This approach of optimizing the light spectrum may be applicable to other species of microalgae.
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제안 방법
The purpose of this study was to test the effect of filtered illumination on the growth and lipid production of Nannochloropsis, as previously reported for monochromatic illumination by blue, red, and green LEDs. Nannochloropsis contains chlorophyll a, β -carotene, and violaxanthin as major pigments [30], and absorbs blue light (400-500 nm) and red light (600-700 nm).
This study investigated the effect of using a simple and inexpensive light filter, based on tar color additives, on the photoautotrophic growth of N. gaditana. Relative to cells grown under white light, filtered light, obtained by a 4:1 mixture red and yellow pigments, improved biomass productivity by 44.
이론/모형
The lipid content was determined using a modified version of the Folch method. The 10 mg freeze-dried biomass sample was mixed with 2 mL of a chloroform-methanol solution (2:1 v/v) in a screw-cap tube and vortexed for 20 min.
성능/효과
In particular, growth under filtered light significantly increased the amount of palmitic acid (C16:0), and significantly decreased the amounts of eicosa pentaenoic acid (EPA, C20:5n3) and tetradecanoic acid (C14:0). As a result, the proportion of saturated fatty acids (SFAs) increased and the proportion of PUFAs decreased, although there was no significant change in the proportion of MUFAs (Fig. 3). Similar results were reported in previous studies of N.
In conclusion, the results presented here indicate that use of an inexpensive pigment solution can mimic the effect of different colored LEDs in increasing lipid content, lipid quality, and biomass productivity of N. gaditana. This strategy of light filtering using different pigments may also improve biodiesel productivity and quality of other microalgal species.
The red pigment had maximal absorption at 506 nm (red line) and the yellow pigment at 455 nm (yellow line). b) A mixture of the red and yellow pigments (4:1 ratio) had broad absorption in the blue region, with half-maximal absorbance at about 550 nm.
후속연구
gaditana. Further studies are needed to determine the optimal spectral composition for FAME production in N. gaditana and other microalgal species, and the applicability of this method in outdoor mass cultivation systems as a cost-effective replacement for artificial light sources.
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