Thi Ngo, Quynh-Mai
(College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
,
Cao, Thao Quyen
(College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
,
Woo, Mi Hee
(College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
,
Min, Byung Sun
(College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
,
Weon, Kwon-Yeon
(College of Pharmacy, Drug Research and Development Center, Daegu Catholic University)
Medicinal plants are potential sources of anticancer agents screening. A large number of phytochemicals, including triterpenoids, have been reported to have significant cytotoxic effects on cancer cells. From the fruits of Ligustrum japonicum Thunb., thirteen triterpenoids (1 - 13) were isolated and...
Medicinal plants are potential sources of anticancer agents screening. A large number of phytochemicals, including triterpenoids, have been reported to have significant cytotoxic effects on cancer cells. From the fruits of Ligustrum japonicum Thunb., thirteen triterpenoids (1 - 13) were isolated and evaluated for their cytotoxic activity against Hela and HL-60 cells. As results, 8 (oleanolic acid) showed significant effects on Hela with $IC_{50}$ values of $5.5{\mu}M$, and moderate effects on HL-60 cells with $IC_{50}$ values of $55.9{\mu}M$. Meanwhile, 10 (oleanderic acid) and 11 ($3{\beta}$-acetoxy-urs-12-en-28-oic acid) exhibited moderate inhibitory effects on Hela with $IC_{50}$ value of 55.0 and $68.8{\mu}M$, respectively. Moreover, 10 showed cytotoxic effect on HL-60 cell line with $IC_{50}$ value of $63.9{\mu}M$. To our knowledge, this is the first report that oleanderic acid was isolated from L. japonicum and investigated in cytotoxic effects on Hela and HL-60 cells.
Medicinal plants are potential sources of anticancer agents screening. A large number of phytochemicals, including triterpenoids, have been reported to have significant cytotoxic effects on cancer cells. From the fruits of Ligustrum japonicum Thunb., thirteen triterpenoids (1 - 13) were isolated and evaluated for their cytotoxic activity against Hela and HL-60 cells. As results, 8 (oleanolic acid) showed significant effects on Hela with $IC_{50}$ values of $5.5{\mu}M$, and moderate effects on HL-60 cells with $IC_{50}$ values of $55.9{\mu}M$. Meanwhile, 10 (oleanderic acid) and 11 ($3{\beta}$-acetoxy-urs-12-en-28-oic acid) exhibited moderate inhibitory effects on Hela with $IC_{50}$ value of 55.0 and $68.8{\mu}M$, respectively. Moreover, 10 showed cytotoxic effect on HL-60 cell line with $IC_{50}$ value of $63.9{\mu}M$. To our knowledge, this is the first report that oleanderic acid was isolated from L. japonicum and investigated in cytotoxic effects on Hela and HL-60 cells.
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제안 방법
Silica gel (Merck, 63 - 200 µm particle size) and RP C-18 (Merck, 75 µm particle size) were used for column chromatography. TLC was performed using Merck silica gel 60 F254 and RP C-18 F254 plates, and spots were visualized by spraying with 10% H2SO4 in ethanol, and then heating.
대상 데이터
A voucher specimen (CUD–14603) was deposited at the Herbarium of the College of Pharmacy, Daegu Catholic University, Korea.
UV spectra were recorded using a Thermo spectrometer. IR spectra were recorded using a JASCO FT/IR-4100 spectrometer. Mass spectra were recorded using an AB SCIEX TripleTOFTM 5600 spectrometer.
japonicum Thunb. used in this study were collected in Gyeongsan province, Republic of Korea in December 2015 and identified by Professor Byung Sun Min. A voucher specimen (CUD–14603) was deposited at the Herbarium of the College of Pharmacy, Daegu Catholic University, Korea.
이론/모형
Cytotoxic activity − The cytotoxic activity assay was carried out using the 3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide (MTT) assay.
참고문헌 (20)
Jensen, S. R.; Franzyk, H.; Wallander, E. Phytochemistry 2002, 60, 213-231.
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