[논문]Effects of standardized extracts of Lamium album and Urtica dioica on rat tracheal smooth muscle contraction

Arefani, Samane; Mehran, Seyyed Mohammad Mohseni; Moladoust, Hassan; Norasfard, Mohammad Reza; Ghorbani, Ahmad; Abedinzade, Mahmood

doi:10.3831/kpi.2018.21.008

Effects of standardized extracts of Lamium album and Urtica dioica on rat tracheal smooth muscle contraction 원문보기

Journal of pharmacopuncture, v.21 no.2, 2018년, pp.70 - 75

Arefani, Samane (Department of Physiology, Faculty of Medicine, Guilan University of Medical Sciences) , Mehran, Seyyed Mohammad Mohseni (Department of Physiology, Faculty of Medicine, Guilan University of Medical Sciences) , Moladoust, Hassan (Department of Biophysics and Biochemistry, Faculty of Medicine, Guilan University of Medical Sciences) , Norasfard, Mohammad Reza (Department of Physiology, Faculty of Medicine, Guilan University of Medical Sciences) , Ghorbani, Ahmad (Pharmacological Research Center of Medicinal Plants, Mashhad University of Medical Sciences) , Abedinzade, Mahmood (Medical Biotechnology Research Center, Faculty of Paramedicine, Guilan University of Medical Sciences)

Abstract ▼ AI-Helper

Objective: Diseases of the respiratory system are one of the main causes of death and include situation such as chronic obstructive pulmonary disease, pneumonia or asthma. Medicinal plants have beneficial effects on multiple diseases include respiratory disorders like asthma and bronchitis. The aim of this study was to evaluate the effects of U. dioica and L. Album on tracheal smooth muscle contraction. Material and methods: Hydroalcoholic extracts of L. Album and U. Dioica aerial parts were prepared by maceration method and standardized based on their total phenol content. The effect of the extracts on the KCl-induced contraction of rat trachea was examined in an organ bath chamber. Data was analyzed with spss software 22. Results: The extract of L. Album (5 mg/ml), similar to theophylline (20 mM), significantly reduced the KCl-induced tracheal contraction. On the other hand, U. Dioica (1 mg/ml and 5 mg/ml) augmented the KCl-induced contraction. Conclusion: The relaxant effect of L. Album on the trachea makes it as a candidate for the managing patients with asthma and obstructive pulmonary diseases. But because of U. Dioica potential constrictive effect on the trachea it is suggested that patients avoid consuming it.

주제어

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문제 정의

Dioica on airway muscle contraction.18 Since there was no available data about the effects of L. Album and U. Dioica extracts on the trachea, the aim of this work was to test the potential relaxant effect of these extracts on the trachea in organ bath condition.

대상 데이터

Male albino Wistar rats (200-250 g) were used in this study. They were kept in a standard room with controlled lighting (12 h light and 12 h darkness) at a temperature of 22 ± 2°C.
1) were collected from Rasht region in Guilan Province, Iran. They were identified by Fatemeh Yousefbeyk, Department of Pharmacognosy, Guilan University of Medical Sciences, Iran (voucher specimen No. 202HGUM and 156HGUM for L. Album and U. Dioica, respectively). For each plant, the hydroalcoholic extract was prepared by macerating the powder of stems and leaves in 50% ethanol (10 ml/g powder) for 72 h at 40 °C.

데이터처리

Data were analyzed with Spss software 22 with One-way analysis of variance and Turkey’s tests.

이론/모형

The extracts were standardized based on their total phenol content by the Folin-Ciocalteu method. A sample of each extract (20 μL of 10 mg/ml) was mixed with the Folin-Ciocalteu reagent (100 μL) and sodium carbonate solution (300 μL, 1 M), and the volume was adjusted to 2 ml with deionized water.

후속연구

25 These controversies may be due to the differences in the method of extraction, parts of the plant used for extraction, animal species, and source of tissues for smooth muscle isolation. In conclusion, the results of the present study suggest that the album has a relaxant effect on the tracheal smooth muscle and might be a candidate for the management of patients with asthma and obstructive pulmonary diseases. Also, it is suggested that these patients, avoid consumption of the U.

참고문헌 (25)

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