Jeong, Sin-Gu
(Department of Biology, College of Natural Science, Chosun University)
,
Lee, Jae-Joon
(Department of Food and Nutrition, College of Natural Science, Chosun University)
,
Kim, Ho-Tae
(Department of Biology, College of Natural Science, Chosun University)
,
Ahn, Min-Ji
(Department of Biology, College of Natural Science, Chosun University)
,
Son, Hee-Kyoung
(Department of Food and Nutrition, College of Natural Science, Chosun University)
,
Lee, Jun Sik
(Department of Biology, College of Natural Science, Chosun University)
,
Oh, Won Keun
(Korea Bioactive Natural Material Bank, Research Institute of Pharmaceutical Science, College of Pharmacy, Seoul National University)
,
Cho, Tae Oh
(Department of Biology, College of Natural Science, Chosun University)
,
Cho, Goang-Won
(Department of Biology, College of Natural Science, Chosun University)
The red algae Gracilaria vermiculophylla is widely spread around seaside areas across the globe, and has been used as a food resource in Southeast Asian countries. Previous studies have shown that Gracilaria red algae extracts have beneficial antihypercholesterolemic, antioxidant, anti-inflammatory,...
The red algae Gracilaria vermiculophylla is widely spread around seaside areas across the globe, and has been used as a food resource in Southeast Asian countries. Previous studies have shown that Gracilaria red algae extracts have beneficial antihypercholesterolemic, antioxidant, anti-inflammatory, and antimicrobial effects. In this study, we investigated the antioxidant effects of Gracilaria vermiculophylla extracts (GV-Ex) on human bone marrow mesenchymal stem cells (hBM-MSCs). The acetone and DMSO/ethanol solvents of the tested GV contain higher total flavonoid and polyphenolic contents that can strongly scavenge reactive oxygen species (ROS). Pre-treatment with GV-Ex protected hBM-MSCs against oxidative stress induced by hydrogen peroxide treatment. The protective effects of GV-Ex treatment were confirmed by MTT assay. The elevated levels of ROS in hBM-MSCs caused by hydrogen peroxide induced oxidative stress were significantly decreased by GV extract treatment. The levels of the antioxidant proteins superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), and catalase (CAT) were also restored or protected by GV-Ex treatment, suggesting that GV extracts moderate excess ROS levels and prevent cells from oxidative damage.
The red algae Gracilaria vermiculophylla is widely spread around seaside areas across the globe, and has been used as a food resource in Southeast Asian countries. Previous studies have shown that Gracilaria red algae extracts have beneficial antihypercholesterolemic, antioxidant, anti-inflammatory, and antimicrobial effects. In this study, we investigated the antioxidant effects of Gracilaria vermiculophylla extracts (GV-Ex) on human bone marrow mesenchymal stem cells (hBM-MSCs). The acetone and DMSO/ethanol solvents of the tested GV contain higher total flavonoid and polyphenolic contents that can strongly scavenge reactive oxygen species (ROS). Pre-treatment with GV-Ex protected hBM-MSCs against oxidative stress induced by hydrogen peroxide treatment. The protective effects of GV-Ex treatment were confirmed by MTT assay. The elevated levels of ROS in hBM-MSCs caused by hydrogen peroxide induced oxidative stress were significantly decreased by GV extract treatment. The levels of the antioxidant proteins superoxide dismutase 1 (SOD1), superoxide dismutase 2 (SOD2), and catalase (CAT) were also restored or protected by GV-Ex treatment, suggesting that GV extracts moderate excess ROS levels and prevent cells from oxidative damage.
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제안 방법
In this study, we examined the anti-oxidative effect of extracts from the red algae G. vermiculophylla using hBM-MSCs.
대상 데이터
G. vermiculophylla was harvested from Busan of Korea (TC17055; deposited in the Marine Brown Algae Resources Bank, Chosun University, Gwangju, Korea), washed three times with tap water to remove residual salts, and dried at room temperature. The seaweed powder was soaked in 80% ethanol (20 times volume) for 7 days, then filtering them through a Qualitative Filter Paper (150 mm diameter; Hyundai Micro, Gyeonggido, Korea).
Cells were incubated one more day at 37oC (reach to 80% confluent), and then treated with GV-Ex for 24 h at 37°C and incubated with 20 μM DCFH-DA at 37°C for 1 h. Images were taken by a DSRl1 digital camera (Nikon).
Total proteins were examined for immunoblot analysis with antioxidant proteins SOD1, SOD2, and CAT. Vehicle is a mixture of equal volumes ethanol and DMSO.
데이터처리
2IC50 values were determined by curve-fitting the data points using nonlinear regression. 3BHA was used as a positive control for DPPH and ABTS radical scavenging activities.
70. After adding 0.5 mL of GV extracts to 3mL of the diluted ABTS solution, the absorbance was measured at 734 nm using a spectrophotometer and the result was calculated in terms of its IC50 values by regression analysis. BHT was used as an ABTS scavenging positive control.
Statistical comparisons between groups were performed using a paired t-test.
Statistical comparisons between groups were performed using a paired t-test. The total polyphenolic and flavonoids contents, DPPH and ABTS radical scavenging activities were statistical analyzed using oneway ANOVA, followed by Tukey test for post hoc comparison.
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