[논문]맥문동(麥門冬)의 증숙(蒸熟)에 따른 항산화 효능 및 고지혈증 유발 흰쥐에 대한 효능 연구

구가람; 이현인; 김수지; 신미래; 이아름; 박해진; 노성수; 서영배

doi:10.6116/kjh.2018.33.5.89.

맥문동(麥門冬)의 증숙(蒸熟)에 따른 항산화 효능 및 고지혈증 유발 흰쥐에 대한 효능 연구
Effects of Steaming Process on Liriopis Tuber to Antioxidant Activities and Hyperlipidemia Induced Rats. 원문보기

大韓本草學會誌 = The Korea journal of herbology, v.33 no.5, 2018년, pp.89 - 103

구가람 (대전대학교 한의과대학 본초학교실) , 이현인 (대전대학교 한의과대학 본초학교실) , 김수지 (대구한의대학교 한의과대학 본초약리학교실) , 신미래 (대구한의대학교 한의과대학 본초약리학교실) , 이아름 (호산대학교 뷰티디자인학과) , 박해진 (대구한의대학교 산학협력단 코스메디컬센터) , 노성수 (대구한의대학교 한의과대학 본초약리학교실) , 서영배 (대전대학교 한의과대학 본초학교실)

Abstract ▼ AI-Helper

Objectives : This study is aimed to compare the changes in Antioxidative capacity of Liriopis Tuber by steaming process and to compare the effects in hyperlipidemia induced rats fed high cholesterol diet between Simvastatin and Liriopis Tuber by steaming process. Methods : The SD rats were divided into six groups: normal diet (Nor), high cholesterol diet (Veh), high cholesterol diet plus Simvastatin 5 mg/kg (Sim), high cholesterol diet plus LT0 extract 200 mg/kg (LT0), high cholesterol diet plus LT6 extract 200 mg/kg (LT6) and high cholesterol diet plus LT9 extract 200 mg/kg (LT9). We compared the total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL), low density lipoprotein cholesterol (LDL) contents and reactive oxygen species (ROS) from each serums. Protein expression in liver tissues related to antioxidant and cholesterol was analyzed. Results : The Antioxidant activity of Liriopis Tuber increased by steaming process. In vivo, TC, TG, LDL-c, atherogenic index (AI) and cardiac risk factor (CRF) decreased and HDL-c increased with increasing steaming frequency. aspartate aminotransferase (AST), alanine aminotransferase (ALT), creatinine, and blood urea nitrogen (BUN) decreased with increasing steaming frequency. ROS decreased only in LT9, and SOD, catalase and glutathione peroxidase (GPx) increased with increasing steaming frequency. phospho-AMP-activated protein kinase (p-AMPK) increased and sterol regulatory element-binding protein 2 (SREBP-2), Phospho-Acetyl-CoA Carboxylase (p-ACC) and HMG-CoA reductase (HMGCR) decreased with increasing steaming frequency. Liver staining showed a decrease in hepatic fat accumulation of LT9. LT9 showed significant results in all experiments. Conclusions : LT9 showed significance of anti-lipid effect and improved fatty liver of hyperlipemia induced rats fed on high cholesterol diet, In conclusion, LP9 can be effectively used for the treatment of hyperlipidemia.

주제어

표/그림 (13)

표 Table 1. The Ingredients of High Cholesterol Diet
그림 Fig 1. Evaluation of antioxidant efficacy of Liriopis Tuber. LT0; Liriopis Tuber without steaming process, LT6; Liriopis Tuber with six times steaming process, LT9; Liriopis Tuber with nine times steaming process.
표 Table 2. Initial Body Weight, Body Weight Gain, Food Intake and Food Efficiency Ratio in High Cholesterol Fed Rats
그림 Fig 2. Liver weights of high cholesterol fed rats Nor; normal diet fed rats, Veh; high cholesterol fed rats, Sim; high cholesterol diet plus simvastatin 5 ㎎/㎏ body weight, LT0; high cholesterol diet plus Liriopis Tuber extract 200 ㎎/㎏ body weight, LT6; high cholesterol diet plus Liriopis Tuber by steaming for six times extract 200 ㎎/㎏ body weight, LT9; high cholesterol diet plus Liriopis Tuber by steaming for nine times extract 200 ㎎/㎏ body weight. All data are expressed mean±SEM, n=7 rats per group. Significance: *p ＜ 0.05, **p ＜ 0.01, ***p ＜ 0.001 vs. high cholesterol fed rats.
그림 Fig 3. Serum analysis of high cholesterol feeding rats Nor; normal diet fed rats, Veh; high cholesterol fed rats, Sim; high cholesterol diet plus simvastatin 5 ㎎/㎏ body weight, LT0; high cholesterol diet plus Liriopis Tuber extract 200 ㎎/㎏ body weight, LT6; high cholesterol diet plus Liriopis Tuber by steaming for six times extract 200 ㎎/㎏ body weight, LT9; high cholesterol diet plus Liriopis Tuber by steaming for nine times extract 200 ㎎/㎏ body weight. All data are expressed mean±SEM, n=7 rats per group. Significance: *p ＜ 0.05, **p ＜ 0.01, ***p ＜ 0.001 vs. high cholesterol fed rats.
그림 Fig 4. Evaluation of AI and CRF in high cholesterol fed rats Nor; normal diet fed rats, Veh; high cholesterol fed rats, Sim; high cholesterol diet plus simvastatin 5 ㎎/㎏ body weight, LT0; high cholesterol diet plus Liriopis Tuber extract 200 ㎎/㎏ body weight, LT6; high cholesterol diet plus Liriopis Tuber by steaming for six times extract 200 ㎎/㎏ body weight, LT9; high cholesterol diet plus Liriopis Tuber by steaming for nine times extract 200 ㎎/㎏ body weight. All data are expressed mean±SEM, n=7 rats per group. Significance: *p ＜ 0.05, **p ＜ 0.01, ***p ＜ 0.001 vs. high cholesterol fed rats.
그림 Fig 5. Evaluation of AST and ALT in high cholesterol fed rats Nor; normal diet fed rats, Veh; high cholesterol fed rats, Sim; high cholesterol diet plus simvastatin 5 ㎎/㎏ body weight, LT0; high cholesterol diet plus Liriopis Tuber extract 200 ㎎/㎏ body weight, LT6; high cholesterol diet plus Liriopis Tuber by steaming for six times extract 200 ㎎/㎏ body weight, LT9; high cholesterol diet plus Liriopis Tuber by steaming for nine times extract 200 ㎎/㎏ body weight. All data are expressed mean±SEM, n=7 rats per group. Significance: *p ＜ 0.05, **p ＜ 0.01, ***p ＜ 0.001 vs. high cholesterol fed rats.
그림 Fig 6. Evaluation of creatinine and BUN in high cholesterol fed rats Nor; normal diet fed rats, Veh; high cholesterol fed rats, Sim; high cholesterol diet plus simvastatin 5 ㎎/㎏ body weight, LT0; high cholesterol diet plus Liriopis Tuber extract 200 ㎎/㎏ body weight, LT6; high cholesterol diet plus Liriopis Tuber by steaming for six times extract 200 ㎎/㎏ body weight, LT9; high cholesterol diet plus Liriopis Tuber by steaming for nine times extract 200 ㎎/㎏ body weight. All data are expressed mean±SEM, n=7 rats per group. Significance: *p ＜ 0.05, **p ＜ 0.01, ***p ＜ 0.001 vs. high cholesterol fed rats.
그림 Fig 8. Antioxidant-related protein expression in liver tissue Nor; normal diet fed rats, Veh; high cholesterol fed rats, Sim; high cholesterol diet plus simvastatin 5 ㎎/㎏ body weight, LT0; high cholesterol diet plus Liriopis Tuber extract 200 ㎎/㎏ body weight, LT6; high cholesterol diet plus Liriopis Tuber by steaming for six times extract 200 ㎎/㎏ body weight, LT9; high cholesterol diet plus Liriopis Tuber by steaming for nine times extract 200 ㎎/㎏ body weight. All data are expressed mean±SEM, n=7 rats per group. Significance: *p ＜ 0.05, **p ＜ 0.01, ***p ＜ 0.001 vs. high cholesterol fed rats.
그림 Fig 7. ROS level of serum in high cholesterol fed rats Nor; normal diet fed rats, Veh; high cholesterol fed rats, Sim; high cholesterol diet plus simvastatin 5 ㎎/㎏ body weight, LT0; high cholesterol diet plus Liriopis Tuber extract 200 ㎎/㎏ body weight, LT6; high cholesterol diet plus Liriopis Tuber by steaming for six times extract 200 ㎎/㎏ body weight, LT9; high cholesterol diet plus Liriopis Tuber by steaming for nine times extract 200 ㎎/㎏ body weight. All data are expressed mean±SEM, n=7 rats per group. Significance: *p ＜ 0.05, **p ＜ 0.01, ***p ＜ 0.001 vs. high cholesterol fed rats.
그림 Fig 9. Results of cholesterol-related protein expression in liver tissue. Nor; normal diet fed rats, Veh; high cholesterol fed rats, Sim; high cholesterol diet plus simvastatin 5 ㎎/㎏ body weight, LT0; high cholesterol diet plus Liriopis Tuber extract 200 ㎎/㎏ body weight, LT6; high cholesterol diet plus Liriopis Tuber by steaming for six times extract 200 ㎎/㎏ body weight, LT9; high cholesterol diet plus Liriopis Tuber by steaming for nine times extract 200 ㎎/㎏ body weight. All data are expressed mean±SEM, n=7 rats per group. Significance: *p ＜ 0.05, **p ＜ 0.01, ***p ＜ 0.001 vs. high cholesterol fed rats.
그림 Fig 10. Liver H&E staining histology. (×100) (A) Nor; normal diet fed rats, (B) Veh; high cholesterol fed rats, (C) Sim; high cholesterol diet plus simvastatin 5 ㎎/㎏ body weight, (D) LT0; high cholesterol diet plus Liriopis Tuber extract 200 ㎎/㎏ body weight, (E) LT6; high cholesterol diet plus Liriopis Tuber by steaming for six times extract 200 ㎎/㎏ body weight, (F) LT9; high cholesterol diet plus Liriopis Tuber by steaming for nine times extract 200 ㎎/㎏ body weight.
그림 Fig 11. Liver oil red O staining histology. (×100) (A) Nor; normal diet fed rats, (B) Veh; high cholesterol fed rats, (C) Sim; high cholesterol diet plus simvastatin 5 ㎎/㎏ body weight, (D) LT0; high cholesterol diet plus Liriopis Tuber extract 200 ㎎/㎏ body weight, (E) LT6; high cholesterol diet plus Liriopis Tuber by steaming for six times extract 200 ㎎/㎏ body weight, (F) LT9; high cholesterol diet plus Liriopis Tuber by steaming for nine times extract 200 ㎎/㎏ body weight.

질의응답

핵심어	질문	논문에서 추출한 답변
	맥문동은 무엇인가?	麥門冬은 백합과 (Liliaceae)에 속하는 다년생 초본인 맥문동 (Liriope platyphylla Wang et Tang) 또는 소엽맥문동 (Ophiopogon japonicus Ker-Gawl.)의 괴근이다1) . 중국에 서는 소엽맥문동 O.
	statin 제제의 부작용에는 어떤 것들이 있는가?	그러나 statin 제제는 다양한 부작용이 보고되고 있다. 가장 흔한 부작용은 근육통으로 약 10%에서 근육통, 위약감 등을 호소하며, 소화장애, 속쓰림, 복통 등이 4% 정도에서 나타나며, 간효소 (AST, ALT) 수치의 상승이 1~2%에서 발생할 수 있고, 인지 장애 또한 보고되고 있다26) . 이러한 statin의 부작용으로 인해 한약 및 천연물로부터의 고지혈증 치료약물 개발을 위한 연구가 많이 이루어지고 있다.
	맥문동의 당뇨와 콜레스테롤에 대한 효능은 무엇인가?	현대에 이르러 서구화되어가는 생활습 관의 변화에 따라 당뇨병, 비만, 대사증후군, 이상지질혈증과 같은 질환이 계속 증가하고 있으며 이는 동맥경화로 인한 혈전성 심혈관계 질환 및 뇌혈관계 질환과 직접적으로 연관되어 있다11) . 이에 麥門冬의 당뇨와 콜레스테롤에 대한 효능 연구 또한 이루어지고 있으며, 혈당과 혈청 콜레스테롤 개선12) , 당뇨 쥐에 대한 항염증 효과13) , PI3-kinase pathway 조절을 통한 인슐린 감작 효과14) , 麥門冬 추출물의 농도가 증가함에 따라 콜레스테롤 감소 효과15) 가 증가함을 보고한 바 있다.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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