[논문]First Report of Allantophomopsiella pseudotsugae Isolated from Soil in Korea

Wajihi, Ally Hassan; Lee, Seung-Yeol; Das, Kallol; Eom, Ahn-Heum; Jung, Hee-Young

doi:10.4489/kjm.20190004

First Report of Allantophomopsiella pseudotsugae Isolated from Soil in Korea 원문보기

한국균학회지 = The Korean journal of mycology, v.47 no.1, 2019년, pp.29 - 34

Wajihi, Ally Hassan (College of Agriculture and Life Sciences, Kyungpook National University) , Lee, Seung-Yeol (College of Agriculture and Life Sciences, Kyungpook National University) , Das, Kallol (College of Agriculture and Life Sciences, Kyungpook National University) , Eom, Ahn-Heum (Department of Biology Education, Korea National University of Education) , Jung, Hee-Young (College of Agriculture and Life Sciences, Kyungpook National University)

Abstract ▼ AI-Helper

A fungal isolate designated 17E029 was isolated from a soil sample in Jeju, Korea. The strain was similar to other Allantophomopsiella species in its morphological characteristics such as grey mycelia, conidiophore, and conidia sizes. The isolate produced aerial mycelia, which appeared grey on the reverse side of the media surfaces and turned black on the front side of the colonies. The conidiophores emanating from the hyphae were hyaline, grey, aseptate, branched, and $6.7{\sim}9.2{\times}1.8{\sim}2.5{\mu}m$. Conidiogenous cells were ovoid to subcylindrical, discrete, guttulate, and hyaline. Conidia were hyaline, aseptate, smooth, guttulate, oval to subcylindrical, irregular in shape, and $6.0{\sim}7.8{\times}3.0{\sim}3.4{\mu}m$. The strain was confirmed based on phylogenetic analysis of the closest related organism, A. pseudotsugae CBS 288.37, using the partial 28S, internal transcribed spacer rDNA regions, and partial RNA polymerase II second largest subunit locus (RPB2) gene sequences along with its culture characteristics. Therefore, morphological observations and phylogenetic analysis revealed that strain 17E029 is similar to the previously identified A. pseudotsugae. Hence, this species was described as A. pseudotsugae strain 17E029, which is a new record in Korea.

주제어

표/그림 (5)

표 Table 1. GenBank codes of fungal strains used for phylogenetic analyses in this study.
표 Table 2. Morphological characteristics of isolate 17E029 compared to the closest species Allantophomopsiella pseudotsugae
그림 Fig. 1. Cultural characteristics of 17E029 (A~F), Colony on PDA (A, B), OA (C, D), and MEA (E, F)showing front and reverse sides.
그림 Fig. 2. Morphological characteristics of 17E029. A, Conidiophores; B, Conidiogenous cells; C, Conidia; Scale bars = 10 μm.
그림 Fig. 3. Neighbour-joining tree based on obtained sequences of the internal transcribed spacer rDNA region, LSU, and partial RNA polymerase II second largest subunit locus (RPB2). Sarcotrochila longispora strain CBS 273.74 was an outgroup. The fungal strain which examined in this study was indicated in bold and the bootstrap value below 70% is not shown. The scale bar, 0.01 indicates the number of nucleotide substitutions.

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문제 정의

This research was supported by a grant from the National Institute of Biological Resources (NIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea for the project on survey and discovery of indigenous fungal species.

대상 데이터

Fungal isolate 17E029 used in this study was collected from a soil sample in Jeju, Korea (33°24'05.3"N, 126°40'31.3"E).
pseudotsugae in Korea. The fungal isolate 17E029 was deposited in the National Institute of Biological Resources (NIBRFG0000502441).

이론/모형

Reference sequences were obtained from GenBank under the accession numbers indicated in Table 1. Alignment and phylogenetic tree construction was conducted by the maximum parsimony method using MEGA 6 software [12] with bootstrap analysis of 1,000 replications.
Table 1. GenBank codes of fungal strains used for phylogenetic analyses in this study.

성능/효과

In this study, different fungal strains were isolated during the observation of unreported fungal species in Korea. Based on its morphological characteristics and results of molecular analysis, the isolated fungus was an undescribed species belonging to the genus Allantophomopsiella. Here, this fungus is identified and illustrated as an unreported species in Korea.
KY676741, respectively. Based on the phylogenetic tree, the representative isolate 17E029 was placed in the clade containing the reference isolate A. pseudotsugae CBS 288.37 with a bootstrap value of 100% in the phylogenetic tree constructed based on a concatenated alignment of the LSU, ITS, and RPB2 sequences (Fig. 3). The phylogenetic analysis results strongly support the fact that the isolate 17E029 is A.
The identity of isolate 17E029 was confirmed by BLAST analysis, which revealed 100% similarity to the partial 28S rDNA sequence of A. pseudotsugae MH871973 and 99% similarity of Phacidium lauri MH871978, 99% similarity to the ITS region sequence of A. pseudotsugae MH857222 and 99% similarity to P. pyri MF375775, 90% and 88% similarities to the partial RPB2 sequence of P. pyri DQ470900 and Allantophomopsis sp. KY676741, respectively.
3). The phylogenetic analysis results strongly support the fact that the isolate 17E029 is A. pseudotsugae, a new record in Korea.

후속연구

pseudotsugae which is a new record in Korea. So, further investigation is necessary to explore the etiology of A. pseudotsugae as well as the pathogenicity based on Korean ecological and environmental conditions.

참고문헌 (13)

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