[논문]고지방식이 동물의 간 조직에서 크리신 투여 또는 중강도 운동이 Inflammasome과 열 발생 유전자발현에 미치는 효과

이영란; 박희근; 이왕록

doi:10.5352/jls.2019.29.5.607

고지방식이 동물의 간 조직에서 크리신 투여 또는 중강도 운동이 Inflammasome과 열 발생 유전자발현에 미치는 효과
The Effects of Either Chrysin or Moderate Exercise on Inflammasome and Thermogenic Markers in High Fat Fed Mice 원문보기

생명과학회지 = Journal of life science, v.29 no.5 = no.229, 2019년, pp.607 - 613

이영란 (전북스포츠과학센터) , 박희근 (충남대학교 스포츠과학과) , 이왕록 (충남대학교 스포츠과학과)

초록
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본 연구 목적은 고지방식이 동물의 간 조직에서 크리신 투여 또는 중강도운동이 Inflammasome과 thermogenesis 유전자 발현의 차이를 규명하고자 시도되었다. 본 연구를 위해 정상식이군, 고지방식이군, 고지방식이+크리신 투여군, 고지방식이+중강도 운동군으로 분류한 후, 크리신 투여군은 16주간 50 mg/kg 농도로 투여하였으며, 운동군은 최대산소섭취량의 60-75%의 중강도 운동으로 실시되었다. 연구결과 크리신 그리고 중강도운동군은 지방조직, 간조직 무게 그리고 지방세포 크기가 고지방식이 군과 비교해 유의하게 감소하였다. Inflammasome 유전자 변화는 크리신 투여군 그리고 중강도 운동군에서 NLRP3. ASC, Casepase1 mRNA 발현이 고지방식이 군과 비교해 유의하게 감소하였다. 열발생마커로 알려진 PGC-1a, BMP7 mRNA 발현은 중강도 운동군에서만 고지방식 이군과 비교해 유의하게 증가했다. 결론적으로 중강도 운동은 고지방식이 동물에서 지방무게, Inflammasome, 그리고 열발생 유전자들의 발현을 비만을 억제하는데 긍정적인 영향을 미치는 것으로 보여진다. 하지만 크리신 투여는 열발생 유전자 발현에는 유의한 차이를 나타내지 못하였다. 향후 연구에서는 크리신의 비만억제 효과를 규명하기 위해 투여농도 기간을 고려한 다양한 연구가 진행되어야 할 것이다.

Abstract ▼ AI-Helper

The purpose of this study was to investigate the effects of either chrysin or exercise on the inflammasome and thermogenic markers in the livers of high-fat fed mice. C57BL/6 mice were randomly assigned to four groups: normal diet control (NC; n=5), high-fat diet control (HC; n=5), high-fat diet with chrysin (Hch; n=5), and high-fat diet with moderate exercise (HME; n=5). The mice were fed a high-fat diet (60% of calories from fat) or normal diet (18% of calories from fat). Chrysin was supplemented orally as 50mg/kg/day dissolved in a 0.1ml solution of dimethyl sulfoxide. The exercised mice ran on a treadmill at 12-20 m/min for 30-60 min/day, 5 times/week, for 16 weeks. After the intervention, the epididymal fat and liver weights were significantly decreased in the HME group compared with HC and Hch groups. The adipocyte size was effectively decreased in the Hch and HME groups compared with the HC group. The inflammasome markers NLRP3, $IL-1{\beta}$, and caspase1 were significantly decreased in the Hch and HME groups compared with the HC group. The thermogenic markers $PGC-1{\alpha}$ and BMP7 were significantly lower in the HC than in the NC group. However, the HME group showed an increase in the thermogenic markers. In conclusion, chrysin and moderate exercise have positive effects on obese metabolic complications induced by high-fat diets by reducing inflammasome genes. However, chrysin supplementation had no effect on thermogenic gene expression. Moderate exercise would therefore seem to be more effective in controlling obesity-induced metabolic deregulation.

주제어

표/그림 (5)

표 Table 1. Primer sequences used for RT-PCR
그림 Fig. 1. The change of (A)epididymal fat ;(B) liver weight of high-fat fed mice.
그림 Fig. 2. The change of adipocyte size of high-fat fed mice.
그림 Fig 3. The change of inflammasome markers of high-fat fed mice.
그림 Fig. 4. The change of thermogenic markers in liver of high-fat fed mice.

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문제 정의

Although most previous studies reported the gainful effects of chrysin and exercise on high-fat-induced metabolic disturbances, there were few results, comparing the effectiveness among them. Therefore, the purpose of this study was to compare the effectiveness either chrysin or moderate exercise training on inflammasome, and browning markers in liver of high fat diet mice.

제안 방법

The other group received the vehicle. Each treatment was administrated once a day, five times a week, for 16 weeks before tissue collection.
, #2018) ad for 16 weeks. The mice were divided into 4 groups: normal diet control (NC; n=5), high fat diet control (HC; n=5), high fat with chrysin (Hch; n=5), high fat with moderate exercise (HME; n=5). Liver tissues were dissected, weighed and immediately frozen.

데이터처리

Statistical analysis of the data was performed by SPSS Version 22.0 using one-way ANOVA with LSD post-hoc tests. Statistical significance was defined as α=.

성능/효과

After 16 weeks of treatments, the epididymal fat, liver weight, and adipocyte size of HC group were significantly increased in comparison with NC group (Fig. 1, Fig. 2). But, HME group was significantly decreased in that’s comparison with HC group.
Male C57BL/6 (Central Experiment Animal, Korea, N=20) mice were housed in cages in a standard experimental laboratory, at temperature 22±2℃, with 60±5% of humidity. After a one-week acclimatization period, the mice were fed a high fat diet (60% of calories from fat, 20% from carbohydrate, 20% from protein, Orient Bio Inc., #D12492) or a normal diet (18% calories from fat, 58% from carbohydrate, 24% from protein, Orient Bio Inc., #2018) ad for 16 weeks. The mice were divided into 4 groups: normal diet control (NC; n=5), high fat diet control (HC; n=5), high fat with chrysin (Hch; n=5), high fat with moderate exercise (HME; n=5).
It will be of interest for further researchers to include. In conclusion, chrysin and moderate exercise have a positive effect on high-fat-diet-induced obese metabolic complication by reducing inflammasome genes. However, there was no effect of chrysin supplementation on the thermogenic gene expression.
In our results, the PGC-1α and BMP7 were significantly decreased in high-fat fed group compared to the normal diet group.
In this study, we found that liver weight and adipocyte size of Hch group were effectively reduced comparison with HC group. But, no significant effect on fat pad weight comparison with HC group.

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