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NTIS 바로가기생명과학회지 = Journal of life science, v.30 no.3, 2020년, pp.291 - 297
동춘매 (국립수산과학원 생명공학과) , 이미난 (국립수산과학원 생명공학과) , 김은미 (국립수산과학원 생명공학과) , 박중연 (국립수산과학원 생명공학과) , 김군도 (국립부경대학교 미생물학과) , 노재구 (국립수산과학원 생명공학과)
This study was conducted to develop microsatellite markers in Seriola quinqueradiata using next-generation sequencing. A total of 28,873,374 reads were generated on an Illumina Hiseq2500 system, yielding 7,247,216,874 bp sequences. The de novo assembly resulted in 466,359 contigs. A total of 132 con...
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핵심어 | 질문 | 논문에서 추출한 답변 |
---|---|---|
방어는 어디에 분포하는가? | 방어(Seriola quinqueradiata)는 농어목(Perciformes) 전갱이과(Carangidae)에 속하는 어종으로 우리나라를 비롯한 일본, 대만 등 북서태평양에 널리 분포하는 온대성 회유어종이다 [25]. 또한 우리나라와 일본 등에서 인기가 많은 고급어종으로 경제적 가치가 높고, 수요가 지속적으로 증가하고 있어 양식 생산을 위한 종묘생산 및 양성에 관한 연구들이 진행되고 있다[13]. | |
방어의 양식 생산을 위한 연구가 진행되고 있는 배경은 무엇인가? | 방어(Seriola quinqueradiata)는 농어목(Perciformes) 전갱이과(Carangidae)에 속하는 어종으로 우리나라를 비롯한 일본, 대만 등 북서태평양에 널리 분포하는 온대성 회유어종이다 [25]. 또한 우리나라와 일본 등에서 인기가 많은 고급어종으로 경제적 가치가 높고, 수요가 지속적으로 증가하고 있어 양식 생산을 위한 종묘생산 및 양성에 관한 연구들이 진행되고 있다[13]. | |
NGS법을 이용하여 microsatellite 마커를 개발한 사례는 무엇인가? | 이를 위해 최근에는 차세대 염기서열(Next-Generation Sequencing, NGS) 분석법을 이용하여 대상어종의 염기서열 정보를 대량으로 확보하고, microsatellite 영역을 직접 탐색함 으로써 microsatellite 마커를 개발하는 방법이 이용되고 있다.[10, 39] 참담치[17], 젓새우[18], 해양미생물[23] 등 다양한 해양생물에서 NGS법을 이용한 microsatellite 마커가 개발되고 있다. |
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