[논문]A new distribution record of Chrysosplenium grayanum Maxim. (Saxifragaceae) in Korea: Evidence from morphological and molecular data

Choi, Ji-Eun; In, Kyung-Ho; Kim, Bong Seok; Kim, Kyeonghee; Kim, Jin-Seok; Kim, Yong-In; Lee, Byoung Yoon; Lim, Chae Eun

doi:10.12651/jsr.2020.9.1.046

A new distribution record of Chrysosplenium grayanum Maxim. (Saxifragaceae) in Korea: Evidence from morphological and molecular data 원문보기

Journal of species research, v.9 no.1, 2020년, pp.46 - 55

Choi, Ji-Eun (National Institute of Biological Resources) , In, Kyung-Ho (Yeonggwang County office) , Kim, Bong Seok (The Society for Korean Peninsula Plants) , Kim, Kyeonghee (National Institute of Biological Resources) , Kim, Jin-Seok (National Institute of Biological Resources) , Kim, Yong-In (Multidisciplinary Genome Institute, Hallym University) , Lee, Byoung Yoon (National Institute of Biological Resources) , Lim, Chae Eun (National Institute of Biological Resources)

Abstract ▼ AI-Helper

Chrysosplenium grayanum Maxim. (Series Nepalensia), which had been known to be restricted to Japan, was newly discovered from Mt. Cheongtae in Yeonggwang-gun, Jeollanam-do, located in the southern part of the Korean Peninsula. Species identification was confirmed using morphological characteristics and DNA sequence data, while comparing with materials obtained from Japan and herbarium specimens. Chrysosplenium grayanum is clearly distinguished from the remaining taxa of the genus Chrysosplenium by having glabrous plant body, opposite leaves, cylindrical papillae with roundish head at the tip on the smooth seed surface, and four stamens. Molecular sequence data of the nuclear ribosomal ITS regions, chloroplast rbcL and matK genes strongly supported that this previously unknown Chrysosplenium species from Korea is C. grayanum. Taking the molecular and the morphological evidence into consideration, it is clear that newly discovered Chrysosplenium population in Korea is conspecific with the widely distributed C. grayanum in Japan. In this paper, we provide a description, illustration, and photo images of Chrysosplenium grayanum from Korea and also a key to the Chrysosplenium species in Korea.

주제어

표/그림 (7)

표 Table 1. List of taxa, voucher specimens, GenBank accession numbers and WIGIS numbers of sequences newly generated during this study. WIGIS is Wildlife Integrated Genetic Information System in National Institute of Biological Resources, Korea.
표 Table 1. Continued.
표 Table 2. Statistics of nrITS, cp rbcL, matK regions, and cpDNA combined data set used in our phylogenetic analyses of Chrysosplenium.
그림 Fig. 1. Illustrations of Chrysosplenium grayanum Maxim.
그림 Fig. 2. Photos of Chrysosplenium grayanum Maxim.
그림 Fig. 3. Scanning electron micrograph of seed of Chysosplenium grayanum Maxim.
그림 Fig. 4. Phylogenetic relationships resulting from the maximum parsimony analysis of nrITS sequences from eight Chrysosplenium taxa and two outgroup taxa (C. flagelliferaum in ser. Flagellifera and C. japonicum in ser. Alternifolia).

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제안 방법

grayanum of Japan and Korea were also consistent with the morphology. Therefore, we confirm the distribution of C. grayanum in Korea based on the evidence from morphological and molecular data, and constructed revised key to identify Korean Chrysosplenium species based on specimens collected from the survey.
01). Thus, to avoid combining different phylogenetic information, nrITS, rbcL, matK, and a combined cpDNA dataset were analyzed, respectively. The statistics of data and phylogenetic analyses for the MP tree and NJ tree are provided in Table 2.

대상 데이터

For molecular identification of the new materials, we obtained nrITS, cp rbcL and matK sequence data for total 30 individuals representing 10 species of Chrysosplenium from Korea and Japan (nine in Korea and two in Japan). Among them, two outgroup taxa [C.
This paper was carried out with the support of the National Institute of Biological Resources(NIBR201729101, NIBA201910101), and authors thank to Dr. Koh Nakamura, Hokkaido University and parataxonomists of The Society for Korean Peninsula Plants (SKPP) who helped collecting samples from Japan and Korea, respectively. We also thank to a NIBR researcher Kim Jung-Hyun, Ryu Se-A, and Lee Wunggi for supporting studies on Chrysosplenium, and Kim Yu-ri, who took photographs of SEM.

이론/모형

html). Phylogenetic analyses were performed using maximum parsimony (MP) and neighbor-joining (NJ) methods. The MP and NJ analysis were implemented in PAUP* 4.

참고문헌 (30)

Bensel, C.R. and B.F. Palser. 1975. Floral anatomy in the Saxifragaceae sensu lato. IV. Baueroideae and conclusions. Am. J. Bot. 62(7):688-694.

상세보기
Chung, T.H. 1965. Illustrated encyclopedia of fauna & flora of Korea. Vol. 5. Tracheophyta. Ministry of Education, Seoul. 232 pp (in Korean).
Chung, Y.H. and Y.D. Kim. 1988. Monographic study of the endemic plants in Korea 10. Taxonomy and interspecific relationships of the genus Chrysosplenium. Korean J. Environ. Biol. 6(2):33-63.
Fay, M.F., K.M. Cameron, G.T. Prance, M.D. Lledo and M.W. Chase. 1997. Familial relationships of Rhabdodendron (Rhadbdodendraceae): plastid rbcL sequences indicate a caryophyllid placement. Kew Bull. 52(4):923-932.

상세보기
Felsenstein, J. 1985. Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39(4):783-791.

상세보기
Han, J.-W., S.-G. Yang, H.-J. Kim, C.-G. Jang, J.-M. Park and S.-H. Kang. 2011. Phylogenetic study of Korean Chrysosplenium based on nrDNA ITS sequences. Korean J. Plant Res. 24(4):358-369.

원문보기 상세보기
Hara, H. 1957. Synopsis of the genus Chrysosplenium L., (Saxifragaceae). J. Fac. Sci. Univ. Tokyo, Bot. 7:1-90.
IUCN. 2019. The IUCN red list of threatened species, version 2019-2 [Available from: http://www.iucnredlist.org, accessed 8 November 2019].
Kim, H.S. and R.S. Kim 1997. Saxifragaceae Dum 1829. In: R.J. Im (ed.), Flora Coreana 3. The Science and Technology Publishing House, Pyongyang. pp. 149-174 (in Korean).
Kim, Y.-D. 2007. Saxifragaceae Juss. In: Flora of Korea Editorial Committee (ed.), The genera of vascular plants of Korea. Academy Publishing Co., Seoul. pp. 521-530.
Kim, Y.-I. and Y.-D. Kim. 2011. Molecular systematic study of Chrysosplenium series Pilosa (Saxifragaceae) in Korea. J. Plant Biol. 54:396-401.

원문보기 상세보기
Kim, Y.-I. and Y.-D. Kim. 2015. Chrysosplenium aureobracteatum (Saxifragaceae), a new species from South Korea. Novon 23:432-436.

상세보기
Kim, Y.-I., S.-H. Cho, J.-H. Lee, D.-H. Kang, J.H. Park and Y.-D. Kim. 2018. Chrysosplenium ramosissimum Y.I. Kim & Y.D. Kim (Saxifragaceae), a new species from Korea. PhytoKeys 111:1-10.

상세보기
Kimura, M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J. Mol. Evol. 16(2):111-120.

상세보기
Lee, T.B. 1980. Illustrated flora of Korea. Hyangmunsa, Seoul. pp. 412-414 (in Korean).
Lee, W.T. 1996. Lineamenta florae Koreae. Academy Publishing Co., Seoul. 1688 pp (in Korean).
Lee, Y.N. 2006. New flora of Korea, Vol. I. Kyohak Publishing Co., Seoul. 974 pp (in Korean).
Lee, Y.N. and Y.C. Oh. 1985. An investigation on flora of Chuwangsan National Park. The Report of the KACN. 23:37-61 (in Korean).
Maximowicz, C.J. 1877. Diagnosis plantarum novarum asiaticarum scripsit. Bull. Acad. Imp. Sci. Saint-Petersbourg 23:340-350.
Nakai, T. 1914. Report of plant research in Jejudo and Wando. Japanese Government-General of Korea, Seoul. 156 pp (in Japanese).
Nakai, T. 1952. A synoptical sketch of Korean flora. Bull. Natl. Sci. Mus. Tokyo 31:1-152.
Nakazawa, M., M. Wakabayashi, M. Ono and J. Murata. 1997. Molecular phylogenetic analysis of Chrysosplenium (Saxifragaceae) in Japan. J. Plant Res. 110:265-274.

상세보기
Park, M.-K. 1974. Keys to the herbaceous plants in Korea (Dicotyledoneae). Chungeumsa, Seoul. 593 pp (in Korean).
Sponberg, G.L. 1972. The genera of Saxifragaceae in the southeastern United States. J. Arnold Arb. 53(4):409-494.
Swofford, D.L. 2002. PAUP*. Phylogenetic analysis using parsimony (*and other methods). Version 4.0.b10. Sinauer Associates Inc., Sunderland.
Thompson, J.D., D.G. Higgins and T.J. Gibson. 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22(22):4673-4680.

상세보기
Wakabayashi, M. 2001. Chrysosplenium L. In: K. Iwatsuki et al. (eds.), Flora of Japan. Vol. IIb. Kodansha, Tokyo. pp. 58-70.
Wakabayashi, M. and H. Takahashi. 1999. A new species of Chrysosplenium (Saxifragaceae) from central Honshu, Japan. Acta Phytotax. Geobot. 50:1-12.
White, T.J., T. Bruns, S. Lee and J. Taylor. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: M.A. Innis, D.H. Gelfand, J.J. Sninsky and T.J. White (eds.), PCR protocols: A guide to methods and applications. Academic Press, Inc., New York. pp. 315-322.
Ye, H. and G. Zhang. 1994. A new species of Chrysosplenium from Guangxi. Acta Bot. Austro Sin. 9:57-59.

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