[논문]콜라겐 단백가수물을 이용한 항노화 펩타이드 제조에 대한 융합 연구

배인영; 한유경; 제현정; 이현준; 이현규

doi:10.15207/jkcs.2020.11.5.061

콜라겐 단백가수물을 이용한 항노화 펩타이드 제조에 대한 융합 연구
Convergence Study on Preparation of Anti-aging Peptides from Fish Collagen Hydrolysates 원문보기

한국융합학회논문지 = Journal of the Korea Convergence Society, v.11 no.5, 2020년, pp.61 - 72

배인영 (극동대학교 식품영양학과) , 한유경 (한양대학교 식품영양학과) , 제현정 (한양대학교 식품영양학과) , 이현준 ((주)농심) , 이현규 (한양대학교 식품영양학과)

초록
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본 연구에서는 어류 유래 콜라겐 가수분해물로부터 한외여과법(MWCO; 1 kDa)과 역상액체크로마토그래피를 이용하여 항노화 활성 기능성 펩타이드를 분리, 정제하고자 하였다. 펩타이드의 분리, 정제에 따른 항노화 활성 변화는 Hs68 세포를 사용하여 procollagen 합성능과 MMP-1 저해능을 측정하여 확인하였다. 콜라겐 가수분해물과 비교하여 최종 분리, 정제된 기능성 펩타이드의 procollagen 합성과 MMP-1 저해는 각각 46%와 77% 향상되었다. 또한 기능성 펩타이드의 구조는 Gly-Arg-Arg-Gly-Asn-Lys (GRRGNK; 콜라겐의 기본 구조인 Gly-X-Y sequence와 유사)의 서열을 보였고, 분자량은 686.175 Da으로 분석되었다. 따라서 본 연구에서는 어류 콜라겐 가수분해물로부터 분리한 펩타이드가 식품, 화장품, 의약품 등의 피부노화 지연 효과를 보이는 기능성 원료로 다양하게 사용할 수 있는 가능성을 확인하였다.

Abstract ▼ AI-Helper

An anti-aging peptide was prepared from fish collagen hydrolysate (FCH) by ultrafiltration (MWCO; 1 kDa) and reversed-phased high performance liquid chromatography (RP-HPLC). Its anti-aging properties were evaluated based on the procollagen-synthesizing and MMP-1-inhibiting activities in Hs68 cells. A potent anti-aging peptide (fraction I-I) increased collagen synthesis by 46% and also inhibited MMP-1 secretion by 77%, compared with unpurified FCH. The amino acid sequence of fraction I-I was identified to be Gly-Arg-Arg-Gly-Asn-Lys (GRRGNK; the repeating Gly-X-Y sequence in collagen), and it had a molecular mass of 686.175 Da. It revealed that the anti-aging activity of GRRGNK was mainly due to skin protective effects. These results demonstrated that fish collagen hydrolysate may be a potential source of anti-aging peptides, which could be utilized in various field, including foods, cosmetics, and pharmaceuticals.

주제어

표/그림 (7)

표 Table 1. Amino acid composition of fish collagen hydrolysate (FCH) (Data citation in the reference of 12)
그림 Fig. 1. Cytotoxicity of Hs68 cells treated with FCHs (Data citation in the reference of 12).
$Fig. 2. Procollagen synthesis by Hs68 cells treated with FCHs and two fractions (1> kDa, <1 kDa) obtained from ultrafiltration.$ 그림 Fig. 2. Procollagen synthesis by Hs68 cells treated with FCHs and two fractions (1> kDa, <1 kDa) obtained from ultrafiltration.
그림 Fig. 3. Purification scheme for obtaining anti-aging peptides from fish collagen hydrolysates.
표 Table 2. Purification of the anti-aging peptide from fish collagen hydrolysate (FCH)
$Table 3. Amino acid composition of the purified peptide (fraction I-I) from fish collagen hydrolysate (FCH) (Data citation in the reference of 12)$ 표 Table 3. Amino acid composition of the purified peptide (fraction I-I) from fish collagen hydrolysate (FCH) (Data citation in the reference of 12)
$Table 4. Anti-aging properties of the purified peptide (fraction I-I) and the synthesized peptide (GRRGNK)$ 표 Table 4. Anti-aging properties of the purified peptide (fraction I-I) and the synthesized peptide (GRRGNK)

AI 본문요약
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문제 정의

Furthermore, collagen peptides from different sources, parts, and food each have distinct amino acid contents, which relate to their anti-aging activities [11]. Therefore, the aim of this study was to isolate and identify an anti-aging peptide from fish collagen hydrolysates (FCHs). Also, we investigated the relation between distinct amino acid contents and anti-aging activities of the anti-aging peptide from FCHs.

제안 방법

The intact mass of the purified peptide was determined by LC/MS analysis using an Agilent 1260 Infinity LC system coupled with an Agilent 6530 Accurate-Mass Q-TOF instrument. The sample (1 uL, 10 mg/mL) was loaded on a Zorbax Eclipse Plus C18 (2.

대상 데이터

Hs68 cells were obtained from the American Type Culture Collection (Manassas, VA, USA). Formic acid and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma-Aldrich Chemical Co.

데이터처리

Data were analyzed by one-way analysis of variance (ANOVA) with Duncan’s test (p<0.05).

이론/모형

The cells were incubated in an atmosphere of 5% CO₂ at 37°C. To evaluate the cytotoxicity of FCHs, cell metabolic activity was measured by the MTT method that is based on the diversion of yellow MTT to purple formazan when the living cells metabolize [13]. A total of 180 uL of cells were added in a 96-well plate at a concentration of 1 x 10⁴ cells/well and incubated for 24 hours at 37°C and 5% CO₂.

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저자의 다른 논문 :

표제어: PCR

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