[논문]건조 스트레스 환경과 스트레스가 없는 환경에서 GM벼와 non-GM벼의 농업 형질 비교

나플라 레이칠; 박재령; 전동원; 김경민

doi:10.5352/jls.2020.30.5.411

건조 스트레스 환경과 스트레스가 없는 환경에서 GM벼와 non-GM벼의 농업 형질 비교
A Comparison Between the Agricultural Traits of GM and Non-GM Rice in Drought Stress and Non-stress Conditions 원문보기

생명과학회지 = Journal of life science, v.30 no.5, 2020년, pp.411 - 419

나플라 레이칠 (경북대학교 농업생명과학대학 응용생명과학부) , 박재령 (경북대학교 농업생명과학대학 응용생명과학부) , 전동원 (경북대학교 농업생명과학대학 응용생명과학부) , 김경민 (경북대학교 농업생명과학대학 응용생명과학부)

초록
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GM 작물의 개발은 경제적 중요성을 갖게 되었고 상업적인 GM 작물을 재배하는 국가들은 1960년대 이후 지속적으로 증가하고 있다. 비록 GM 작물의 경작과 상업화는 경제적 중요성을 얻었지만, 여전히 non-GM 작물에 비해 그들의 농업적 특성을 평가할 필요가 있다. 본 연구는 유전공학에서 사용된 방법의 결과로 발생할 수 있는 의도하지 않은 문제 발생 여부를 확인하기 위해 내건성 유전자 CaMsrB2를 포함한 GM 쌀과 non-GM 쌀의 농업적 특성을 내건성 온실과 관개수답에서 평가했다. 관개수답에서는 GM벼와 non-GM벼의 모든 농업형질에서 유의미한 차이가 없었다. 그러나 내건성온실에서 수수와 수량에서 GM벼와 non-GM벼에서 유의미한 차이가 있었다. 따라서 본 연구 결과는 CaMsrB2 유전자를 함유한 GM벼가 내건성 조건에서 non-GM 쌀에 비해 경제적 가치가 우수하다는 것을 시사한다. 이 결과는 또한 CaMsrB2 유전자를 함유한 GM 벼는 가뭄에 취약한 지역에서 안정적으로 수량을 유지 하면서 재배 가능 하다.

Abstract ▼ AI-Helper

The development of GM crops has gained significant economic importance, and the number of countries cultivating commercial GM crops has continuously increased since the 1960s. Globally, the area given to cultivating GM soybean, maize, cotton, and canola alone had reached 114 million hectares by 2007. Although the economic importance of cultivating and commercializing GM crops has increased, there is still a need to assess their agricultural traits in comparison to non-GM produce. This study evaluated the agricultural traits of GM rice containing the drought-tolerant gene CaMsrB2 and standard rice to investigate any unintended effects of genetic engineering. The GM and non-GM rice were compared in terms of various agricultural traits in a drought greenhouse and an irrigated paddy field. There was no statistical difference in the field-grown crops, but there was a statistically significant difference in both tiller number and yield in the greenhouse. These results therefore suggest that GM rice lines containing the CaMsrB2 gene are superior in performance to non-GM rice in drought stress conditions and could be grown in drought-prone areas where drought intolerant rice may not be able to grow.

주제어

표/그림 (7)

그림 Fig. 1. Phenotype comparison of GM (HV23 and HV8) and non-GM (Ilmi, Samgang, Baekjinju, Junam, Nagdong, Ilpum) rice in drought green house.
그림 Fig. 2. Heading date, culm length, panicle length, tiller number and yield of GM rice (HV23 and HV8) and non-GM rice (Ilmi, Samgang, Baekjinju, Junam, Nagdong, Ilpum) in drought green house for 3 years 2017, 2018, 2019 Comparative analysis.
그림 Fig. 3. Heading date, culm length, panicle length, tiller number and yield of GM rice (HV23 and HV8) and non-GM rice (Ilmi, Samgang, Baekjinju, Junam, Nagdong, Ilpum) in irrigated paddy field for 3 years 2017, 2018, 2019 Comparative analysis.
표 Table 1. Comparison of agricultural traits between GM and non-GM rice in drought green house and irrigated paddy field in 2017
표 Table 2. Comparison of agricultural traits between GM and non-GM rice in drought green house and irrigated paddy field in 2018
표 Table 3. Comparison of agricultural traits between GM and non-GM rice in drought green house and irrigated paddy field in 2019
표 Table 4. Comparative analysis of agricultural traits between GM and non-GM rice in drought green house and irrigated paddy field for 3 years 2017, 2018, 2019

AI 본문요약
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문제 정의

For the cultivation of GM rice to advance, transgenic rice should be evaluated in the agricultural environment and the potential concerns should be assessed [21]. Since the effect of a GM plant is unpredictable, the objective of this study was to evaluate the drought tolerant GM rice lines with their non-GM parent in the different agronomic traits under the drought stress treatment carried out in an automated greenhouse and the irrigation treatment carried out in the paddy field. CaMsrB2 gene is known to provide resistance to biological and abiotic environmental stresses.

제안 방법

It was inoculated in 33℃ dark condition for 3 days, and then seeded in tray. After growing for 4 weeks in the tray, and transplanted to the drought greenhouse and the irrigated paddy field to compare the characteristics of GM rice and Non-GM rice. Each line was planted at a planting density of 30×15 cm, one per line per plant, and herbicide and insecticide spraying, pest control, and package management were cultivated at the county test site in accordance with the RDA standard rice cultivation method.
This study used two fixed drought tolerant GM lines (HV8 and HV23) of T₈ generation containing drought tolerant gene CaMsrB2 in chromosome 1 and chromosome 8 respectively, and a non-drought tolerant variety Ilmi in evaluation of the agronomic traits of the two drought GM rice pedigree over a period of three years/three seasons starting from 2017 to 2019 under two treatments. The CaMsrB2 gene obtained from Capsicum annuum is commended as a novel defense regulator against oxidative stress and pathogen attack [28].

대상 데이터

The experiment was carried out at Kyungpook National University experimental area in Gunwi GM plots (4,700 m2, 360 6´ 41.54´ ´ N, 1280 38´ 26.17´ ´E), where both the greenhouse, and the fields were used to experiment.

데이터처리

*Mean ± SD, Means followed by a common letter are not significantly different at the 5% level by Duncan Multiple Range Test (DMRT).
Data for heading date, culm length, panicle length and tiller number and yield were analyzed using Statistical Package for the Social Sciences (SPSS) 20 statistical package to generate analysis of variance (ANOVA) and significant differences were identified by Duncan’s multiple range test (DMRT) at 0.05.
Means denoted by the same letter are not significantly different (p>0.05) as evaluated by Duncan Multiple Range Test (DMRT).

성능/효과

In order to confirm the stable expression and excellence of gene function in the GM rice, HV8, HV23 which have a drought-tolerant gene CaMsrB2 inserted in Ilmi, and six representative varieties in Korea (Ilmi, Samgang, Baekjinju, Junam, Nagdong, Ilpum) agricultural traits were compared (Fig. 1). Since HV23 and HV8 have inserted the drought tolerant gene CaMsrB2, all varieties used in this research were grown in drought-green house and irrigation paddy yields (Fig.

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