[논문]Chemical Fungicides and Bacillus siamensis H30-3 against Fungal and Oomycete Pathogens Causing Soil-Borne Strawberry Diseases

Park, Bo Reen; Son, Hyun Jin; Park, Jong Hyeob; Kim, Eun Soo; Heo, Seong Jin; Youn, Hae Ree; Koo, Young Mo; Heo, A Yeong; Choi, Hyong Woo; Sang, Mee Kyung; Lee, Sang-Woo; Choi, Sung Hwan; Hong, Jeum Kyu

doi:10.5423/ppj.nt.12.2020.0232

Chemical Fungicides and Bacillus siamensis H30-3 against Fungal and Oomycete Pathogens Causing Soil-Borne Strawberry Diseases 원문보기

The plant pathology journal, v.37 no.1, 2021년, pp.79 - 85

Park, Bo Reen (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) , Son, Hyun Jin (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) , Park, Jong Hyeob (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) , Kim, Eun Soo (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) , Heo, Seong Jin (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) , Youn, Hae Ree (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) , Koo, Young Mo (Department of Plant Medicine, Andong National University) , Heo, A Yeong (Department of Plant Medicine, Andong National University) , Choi, Hyong Woo (Department of Plant Medicine, Andong National University) , Sang, Mee Kyung (National Institute of Agricultural Sciences, Rural Development Administration) , Lee, Sang-Woo (Department of Horticultural Science, Gyeongnam National University of Science and Technology (GNTech)) , Choi, Sung Hwan (Department of H) , Hong, Jeum Kyu

Abstract ▼ AI-Helper

Chemical and biological agents were evaluated to inhibit Colletotrichum fructicola, Phytophthora cactorum, and Lasiodiplodia theobromae causing strawberry diseases. Mycelial growths of C. fructicola were gradually arrested by increasing concentrations of fungicides pyraclostrobin and iminoctadine tris (albesilate). P. cactorum and L. theobromae were more sensitive to pyraclostrobin compared to C. fructicola, but iminoctadine tris (albesilate) was not or less effective to limit P. cactorum or L. theobromae, respectively. Bacillus siamensis H30-3 was antagonistic against the three pathogens by diffusible as well as volatile molecules, and evidently reduced aerial mycelial formation of P. cactorum. B. siamensis H30-3 growth was declined by at least 0.025 mg/ml of pyraclostrobin. The two fungicides additively inhibited mycelial growths of C. fructicola, but not of P. cactorum and L. theobromae. B. siamensis H30-3 volatiles led to less growth of C. fructicola than one reduced by the fungicides. Taken together, in vitro antimicrobial activities of the two fungicides together with or without B. siamensis H30-3 volatiles may be cautiously incorporated into integrated management of strawberry diseases dependent on causal pathogens.

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표/그림 (5)

그림 Fig. 1. In vitro inhibitory efficacies of two chemical fungicides pyraclostrobin and iminoctadine tris (albesilate) on mycelial growths of Colletotrichum fructicola isolate SAn-3, Phytophthora cactorum isolate P-9815 (KACC 40183) and Lasiodiplodia theobromae isolate LT120902. (A) Colony formations of C. fructicola, P. cactorum, and L. theobromae on 1/2-potato dextrose agar media containing different concentrations of pyraclostrobin and iminoctadine tris (albesilate), and cultured at 25℃ for 5, 10, and 2 days for C. fructicola, P. cactorum, and L. theobromae, respectively. (B) Relative mycelial growths (%) of C. fructicola, P. cactorum, and L. theobromae. Bars represent the standard errors of the means of the five independent experimental replications. Each experiment has four replications. Means followed by the same letters are not significantly different at 5% level by least significant difference test.
그림 Fig. 2. In vitro inhibition of mycelial growths of Colletotrichum fructicola, Phytophthora cactorum, and Lasiodiplodia theobromae by dual cultures with Bacillus siamensis H30-3. (A) Mycelial cultures on 1/2-potato dextrose agar media in the absence or presence of B. siamensis H30-3 co-culture at 25℃. Photos were taken 8, 16, and 4 days after inoculation of C. fructicola, P. cactorum, and L. theobromae, respectively. (B) Inhibited mycelial growths shown by half of the colony diameter after co-culture. Bars represent the standard errors of the means of the five independent experimental replications. Each experiment has four replications. Asterisks indicate significant differences as determined by Student's t-test (P < 0.05).
그림 Fig. 3. In vitro inhibition of mycelial growths of Colletotrichum fructicola, Phytophthora cactorum, and Lasiodiplodia theobromae by Bacillus siamensis H30-3 volatiles. (A) Schematic diagram showing antimicrobial volatiles from B. siamensis H30-3 against phytopathogens. Nine drops (5 μl each) of B. siamensis H30-3 suspension (10⁸ cfu/ml) were inoculated on tryptic soy agar (TSA), nutrient agar (NA), and Luria-Bertani agar (LBA). Arrows indicate releasing volatiles onto mycelia of the phytopathogens. (B) Pathogen cultures on 1/2-potato dextrose agar (PDA) media in the absence and presence of B. siamensis H30-3 volatiles on the three different growth media at 25℃. Photos were taken 5, 10, and 2 days after inoculation of C. fructicola, P. cactorum, and L. theobromae, respectively. (C) Colony diameters (mm) of C. fructicola, P. cactorum, and L. theobromae treated with B. siamensis H30-3 volatiles. Bars represent the standard errors of the means of the six independent experimental replications. Each experiment has four replications. Asterisks indicate significant differences as determined by Student's t-test (P < 0.05).
그림 Fig. 4. Effects of pyraclostrobin and iminoctadine tris (albesilate) on in vitro growth of Bacillus siamensis H30-3. Bacterial suspensions in nutrient broth liquid media with or without different concentrations of the two fungicides were cultured and relative bacterial growths in response to the two fungicides were demonstrated as percentage (%) compared to those in untreated culture. Values presented are means and error bars indicated the standard errors of the means of five independent experimental replications. Each experiment has four replications. Means followed by the same letters are not significantly different at 5% level by least significant difference test.
그림 Fig. 5. Antimicrobial activities of chemical fungicides pyraclostrobin and iminoctadine tris (albesilate) with or without volatiles from Bacillus siamensis H30-3. Mycelial growths of Colletotrichum fructicola, Phytophthora cactorum and Lasiodiplodia theobromae on 1/2-potato dextrose agar (PDA) media supplemented with pyraclostrobin (P) (0.00625 mg/ml) and/or iminoctadine tris (albesilate) (I) (0.00625 μg/ml). The PDA media were covered by tryptic soy agar media inoculated by B. siamensis H30-3. Colony diameters were measured after cultures at 25℃ for 5, 10, and 2 days for C. fructicola, P. cactorum and L. theobromae, respectively. Values presented are means and error bars indicated the standard errors of the means of five independent experimental replications. Each experiment has four replications. Means followed by the same letters are not significantly different at 5% level by least significant difference test.

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theobromae, respectively. (B) Inhibited mycelial growths shown by half of the colony diameter after co-culture. Bars represent the standard errors of the means of the five independent experimental replications.

데이터처리

Each experiment has four replications. Asterisks indicate significant differences as determined by Student’s t-test (P < 0.05).
Each experiment has four replications. Asterisks indicate significant differences as determined by Student’s t-test (P < 0.05).

성능/효과

In this study, in vitro antifungal activities of two fungicides pyraclostrobin and iminoctadine tris (albesilate) were demonstrated against C. fructicola, P. cactorum, and L. theobromae by singly or simultaneously. B.
1 µg/ ml. These results suggest that pyraclostrobin can be applied to control both Phytophthora rot and dieback caused by P. cactorum and L. theobromae, respectively. Antimicrobial activities of iminoctadine tris (albesilate) by more than 0.

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