[논문]참당귀 세미 추출물의 미백 및 항산화 효과

이상훈; 박정용; 서경혜; 최장남; 이윤정; 문윤호; 허목; 박우태; 허윤찬; 장재기; 구성철

doi:10.7732/kjpr.2021.34.1.037

참당귀 세미 추출물의 미백 및 항산화 효과
Whitening and Antioxidant Effects of Extracts from Angelica gigas Nakai Thin Root 원문보기

韓國資源植物學會誌 = Korean journal of plant resources, v.34 no.1, 2021년, pp.37 - 43

이상훈 (농촌진흥청 국립원예특작과학원 약용작물과) , 박정용 (농촌진흥청 국립원예특작과학원 인삼특작이용팀) , 서경혜 (농촌진흥청 국립원예특작과학원 화훼과) , 최장남 (농촌진흥청 국립원예특작과학원 약용작물과) , 이윤정 (농촌진흥청 국립원예특작과학원 약용작물과) , 문윤호 (농촌진흥청 국립원예특작과학원 약용작물과) , 허목 (농촌진흥청 국립원예특작과학원 약용작물과) , 박우태 (농촌진흥청 국립원예특작과학원 약용작물과) , 허윤찬 (농촌진흥청 국립원예특작과학원 약용작물과) , 장재기 (농촌진흥청 국립원예특작과학원 약용작물과) , 구성철 (농촌진흥청 국립원예특작과학원 약용작물과)

초록
AI-Helper

본 연구는 참당귀 가공 시에 버려지는 부산물인 세미를 기능성 화장품 원료로 활용가능성이 있는지 평가하기 위해 수행하였다. MTS assay 수행결과 70% 주정을 이용한 뿌리 부위별 추출물의 경우 처리농도 25 ~ 200 ㎍/mL까지 세포독성이 없었다. 추출물 200 ㎍/mL를 처리하였을 때의 melanin 생성 억제효과는 추출 부위별로 12% ~ 19%로 나타났으며, 세미와 약재의 melanin 생성 억제효과가 비슷하게 나타났다. 그리고 DPPH radical 소거능이 50%에 달하는 IC50 값은 세미 677.9 ± 30.5 ㎍/mL, 약재 728.0 ± 42.4 ㎍/mL로 나타났고, ABTS radical 소거능이 50%에 달하는 IC50 값은 세미 114.9 ± 0.1 ㎍/mL, 약재 173.6 ± 1.3 ㎍/mL으로 나타나 세미가 약재보다 항산화활성이 높게 나타났다. 따라서 본 실험결과를 고려해 봤을 때, 부산물인 세미는 기능성 화장품 원료로 이용 가능성이 높은 것으로 생각된다.

Abstract ▼ AI-Helper

Angelica gigas Nakai (AGN) is a perennial plant belonging to the family Apiaceae. Its root has been utilized as a traditional medicine especially in Korea. This study was carried out to evaluate the potential use of extracts from AGN root parts as a cosmetic material. The dried AGN roots are divided into body (B), thick root (TkR), medium root(MR) and thin root (TnR) according to their diameter before cutting into medicine. B, TkR and MR of AGN are combined and used as medicinal herbs (MH). The extracts from AGN each root part (B, TkR, MR, TnR, MH) were used to test the effect on cell viability using MTS assay and to examine inhibitory effect on melanin accumulation in B16F10 melanoma cells. All extracts (50 - 200 ㎍/mL) from the each root part did not affect the cell viability. And inhibitory effect of all root extracts (200 ㎍/mL) on melanin accumulation was 12-19%. Especially, TnR showed similar inhibitory effect on melanin accumulation to MH. In addition, DPPH and ABTS free radical scavenging activity were higher in the TnR extract compared to MH. This study showed that the TnR extract exhibit high inhibitory effect on melanin accumulation and antioxidant activity compared to MH, suggesting that TnR extract has potential as a cosmetic ingredient.

주제어

표/그림 (5)

그림 Fig. 1. Cell viability of Angelica gigas extracts on B16F10 melanoma cells. (A): thick root extract, (B): thin root extract, (C): medium root extract, (D): root body extract, (E): medicinal herb extract. Cell viability was examined using MTS assay. Each experiment was conducted in triplicates, and the results are means ± SD.
그림 Fig. 2. Inhibitory effect of Angelica gigas extracts on melanin accumulation in B16F10 melanoma cells. B16F10 melanoma cells were treated with IBMX (100 μM) and root extracts of 200 ㎍/mL for 48hours. TkR, TnR, MR, B, MH indicates thick root extract, thin root extract, medium root extract, body extract, medicinal herb extract, respectively. The results are means ± SD. *Means with different letters are significant at p < 0.05 by Duncan's Multiple Range Test (DMRT).
표 Table 1. Inhibitory effect on the melanogenesis of Angelica gigas root parts
표 Table 2. DPPH free radical scavenging activity of Angelica gigas root parts
표 Table 3. ABTS free radical scavenging activity of Angelica gigas root parts

참고문헌 (29)

Bae, W.J., J.S. Ha, J.B. Choi, K.S. Kim, S.J. Kim, H.J. Cho, S.H. Hong, J.Y. Lee, Z. Wang, S.Y. Hwang and S.W. Kim. 2016. Protective effect of decursin extracted from Angelica gigas in male infertility via Nrf2/HO-1 signaling pathway. Oxid. Med. Cell. Longev. doi: 10.1155/2016/5901098.

상세보기
Baik, M., J.H. Kim, D.W. Lee, J.S. Hwang and E. Moon. 2017. Anti-aging cosmetic application of novel multi-herbal extract composed of Nelumbo nucifera leaves, Saururus chinensis and Orostachys japonica. J. Soc. Cosmet. Sci. Korea 43(2):93-102.

원문보기 상세보기
Jang, M.J., S.J. Cheon, H.Y. Kim, D.J. Kwoen, H.Y. Kim, S.H. Kim and J.T. Lee. 2011. The anti-wrinkle and whitening effect of extracts of Castanea crenata inner shell. J. Life Sci. 21(5):734-738.

원문보기 상세보기
Ju, J.I, J. Lee, S.W. Paik, T.S. Yun, Y.C. Park, B.H. Lee, H.H. Kim and H.B. Lee. 2015. Effect of drying temperature on high quality functional processed products of Chinese matrimony vine. Korean J. Medicinal Crop Sci. 23(6):468-472.

원문보기 상세보기
Kang, S.Y., K.Y. Lee, M.J. Park, Y.C. Kim, G.J. Markelonis and T.H. Oh. 2003. Decursin from Angelica gigas mitigates amnesia induced by scopolamine in mice. Neurobiol. Learn. Mem. 79(1):11-18.

상세보기
Kim, B.K., I.U. Hwang, Y.H. Hwang, M.J. Bae, S.M. Kim and J.H. An. 2002. Effects of dietary Panax ginseng leaves, Dioscorea japonica hulls and oriental medicine refuse on physico-chemical properties of Korean native chicken meat. Korean J. Food Sci. An. 22(2):1 22-129.
Kim, C.H., M.C. Kwon, J.C. Han, C.S. Na, H.G. Kwak, G.P. Choi, U.Y. Park and H.Y. Lee. 2008. Skin-whitening and UV protective effects of Angelica gigas Nakai extracts on ultra high pressure extraction process. Korean J. Medicinal Crop Sci. 16(4):255-260.
Kim, D.S., D.H. Kim, M.J. Oh, K.G. Lee, M.C. Kook and C.S. Park. 2010a. Antiaging and whitening activities of ehthanol extract of yuza (Citrus junos Sieb ex Tanaka) by product. J. Soc. Cosmet. Sci. Korea 36(2):137-143.
Kim, J., M. Yun, E.O. Kim, D.B. Jung, G. Won, B. Kim, J.H. Jung and S.H. Kim. 2016. Decursin enhances TRAIL-induced apoptosis through oxidative stress mediated-endoplasmic reticulum stress signalling in non-small cell lung cancers. Br. J. Pharmacol. 173:1033-1044.

상세보기
Kim, S.Y., C.R. Kim, H.M. Kim, M. Kong, J.H. Lee, H.J. Lee, M.S. Lim, N.R. Jo and S.N, Park. 2010b. Antioxidant activity and whitening effect of Cedrela sinensis A. Juss shoots extracts. J. Soc. Cosmet. Sci. Korea 36(3):175-182.
Kim, Y.A., S.H. Park, B.Y. Kim, A.H. Kim, B.J. Park and J.J. Kim. 2014. Inhibitory effects on melanin production of demethylsuberosin isolated from Angelica gigas Nakai. Kor. J. Pharmacogn. 45(3):209-213.
Kim, Y.S., H.J. Park, D.H. Lee and H.K. Kim. 2018. Development of multiples polymerase chain reaction assay for identification of Angelica species. Korean J. Medicinal Crop Sci. 26(1):26-31.

원문보기 상세보기
Lee, H.J. and S.N. Park. 2011. Antioxidative effect and active component analysis of Quercus salicina Blume extracts. J. Soc. Cosmet. Sci. Korea 37(2):143-152.

원문보기 상세보기
Lee, H.J., S.W. Lee, C.G. Park, Y.S. Ahn, J.S. Kim, M.S. Bang, C.H. Oh and C.T. Kim. 2015. Effects of white Habiscus syriacus L. flower extracts on antioxidant activity and bone resorption inhibition. Korean J. Medicinal Crop Sci. 23(3):190-197.

원문보기 상세보기
Lee, M.J., S.J. Lee, H.R. Choi, J.H. Lee, J.W. Kwon, K.S. Chae, J.T. Jeong and T.B. Lee. 2014. Improvement of cholesterol and blood pressure in fruit, leaf and stem extracts from black raspberry in vitro. Korean J. Medicinal Crop Sci. 22(3):177-187.

원문보기 상세보기
Lee, S., Y.S. Lee, S.H. Jung, K.H. Shin, B.K. Kim and S.S. Kang. 2003. Antioxidant activities of decursinol angelate and decursin from Angelica gigas roots. Nat. Prod. Sci. 9(3):170-173.
Lee, S.H., C.O. Hong, M. Hur, J.W. Han, W.M. Lee, Y. Lee and S.C. Koo. 2019a. Evaluation of the availability of bolting Angelica gigas Nakai. Korean J. Plant Res. 32(4):318-324.
Lee, S.H., M. Jin, C.O. Hong, M. Hur, J.W. Han, W.M. Lee, H.M. Yun, Y.B. Kim, Y. Lee and S.C. Koo. 2019b. Analysis of index component content and antioxidant activity according to the root diameter of Angelica gigas Nakai. Korean J. Plant Res. 32(2):116-123.

원문보기 상세보기
Lee, S.M. 2019. Antimelanogenic effect of isomaltol glycoside from red ginseng extract. J. Soc. Cosmet. Sci. Korea 45(3):255-263.

원문보기 상세보기
Lee, S.W., S.J. Lee, E.H. Han, E.C. Sin, K.M. Cho and Y.H. Kim. 2017. Current status on the development of molecular markers for differentiation of the origin of Angelica spp. J. Plant Biotechnol. 44:12-18 (in Korean).

원문보기 상세보기
Lee, S.Y. and J.Y. Lee. 2013. Inhibitory efficacy of Angelica gigas Nakai on microphthalmia-associated transcription factor (MITF), tyrosinase related protein-1 (TRP-1), tyrosinase related protein-2 (TRP-2), and tyrosinase mRNA expression in melanoma cells (B16F10). J. Life Sci. 23(11):1336-1341.

원문보기 상세보기
Ministry of Agriculture, Food and Rural Affairs (MAFRA). 2019. 2018 an actual output of crop for a special purpose. Ministry of Agriculture, Food and Rural Affairs. Sejong, Korea. pp. 83-85.
Park, H.Y., S.B. Kwon, N.K. Heo, W.J. Chun, M.J. Kim and Y.S. Kwon. 2011. Constituents of the stem of Angelica gigas with rat lens aldose reductase inhibitory activity. J. Korean Soc. Appl. Bi. 54(2):194-199.
Park, J.C., Y.H. Kim, H.J. Jung, S.Y. Ji, S.D. Lee, J.W. Ryu, H.D. Jang, H.K. Moon and I.C. Kim. 2007. Effects of dietary ginseng by-product on growth performance and pork quality parameters in finishing pigs. J. Anim. Sci. Technol. 49(6):839-846.

원문보기 상세보기
Park, S.J. and H.Y. Lee. 2015. Component analysis and antioxidant activity of Wasabi japonica Matsum leaves. Korean J. Medicinal Crop Sci. 23(3):207-213.

원문보기 상세보기
Yim, D., R.P. Singh, C. Agarwal, S. Lee, H. Chi and R. Agarwal. 2005. A novel anticancer agent, decursin, induces G1 arrest and apoptosis in hunman prostate carcinoma cells. Cancer Res. 65(3):1035-1044.
Yoo, Y.M., J.N. Ahn, H.S. Chea, B.Y. Park, J.H. Kim, J.M. Lee, Y.K. Kim and H.K. Park. 2004. Characteristic of pork quality during storage fed with ginseng by-products. Korean J. Food Sci. An. 24(1):37-43.
Yoon, M.Y., Y.S. Kim, G.J. Choi, K.S. Jang, Y.H. Choi, B. Cha and J.C. Kim. 2011. Antifungal activity of decursinol angelate isolated from Angelica gigas roots against Puccinia recondita. Res. Plant Dis. 17(1):25-31.

원문보기 상세보기
Yu, H.S., C.H. Park, C.G. Park, Y.G. Kim, H.W. Park and N.S. Seong. 2004. Growth characteristics and yield of the three species of genus Angelica. Korean J. Medicinal Crop Sci. 12(1):43-46.

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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