[논문]단삼 메탄올 추출물의 구강 병원성 세균에 대한 항균 및 항염증효과

이정혁; 임동술; 최성숙

doi:10.22889/kjp.2021.52.1.41

단삼 메탄올 추출물의 구강 병원성 세균에 대한 항균 및 항염증효과
Antibacterial Activity and Anti-inflammatory Effect of Methanol Extracts of Saliva miltiorrhiza Against Oral Pathogenic Bacteria 원문보기

생약학회지, v.52 no.1, 2021년, pp.41 - 48

이정혁 (삼육대학교 약학대학) , 임동술 (삼육대학교 약학대학) , 최성숙 (삼육대학교 약학대학)

Abstract ▼ AI-Helper

This research was conducted to investigate the antibacterial and anti-inflammatory effects of MeOH Ex. of Salvia miltiorrhiza (MESM) against oral pathogenic bacteria. Minimum inhibitory concentration (MIC), removal effect of biofilm produced by Streptococcus mutans, effect of gene expression of proinflammatory cytokines and effect of production of proinflammatory cytokine of MESM were tested. MESM showed moderated antibacterial activity against oral pathogenic bacteria. About 89±8% of biofilms produced by S. mutans were removed by MESM at a concentration of 1 mg/mL. Gene expression of IL-1β and TNF-α induced by Porphyromonas gingivalis were 8~9 folds reduced by MESM. Gene expression of IL-8 induced by Fusobacterium nucelatum were 12 folds reduced by MESM. Production of IL-1β, TNF-α and IL-8 were significantly suppressed by MESM. Conclusively, MESM showed potent antibacterial and anti-inflammatory effect against oral pathogenic bacteria.

주제어

표/그림 (7)

표 Table I. Primer sequences for qRT-PCR analysis
그림 Fig. 1. Biofilm removal effect of MeOH Ex. of S. miltiorrhiza (MESM). Values are presented as mean±S.D. *p<0.05, **p< 0.01 compared with control.
그림 Fig. 2. Cytotoxicity of MeOH Ex. of S. miltiorrhiza (MESM) on YD-38 cells. Values are presented as mean±S.D. *p<0.05, **p<0.01 compared with control.
그림 Fig. 3. Effect of MeOH Ex. of S. miltiorrhiza (MESM) on Pg LPS induced expression of IL-1β and TNF-α mRNA in YD- 38 cells. (A) Fold changes in IL-1β mRNA expression. (B) Fold changes in TNF-α mRNA expression. Values are pre-sented as mean±S.D. *p<0.05, **p<0.01 compared with control.
표 Table II. Antibacterial activity of MeOH Ex. of S. miltiorrhiza
그림 Fig. 4. Effect of MeOH Ex of S. miltiorrhiza (MESM) on F. nucleatum (FN) induced expression of IL-6 and IL-8 mRNA in YD-38 cells. (A) Fold changes in IL-6 mRNA expression. (B) Fold changes in IL-8 mRNA expression. Values are pre-sented as mean±S.D. *p<0.05, **p<0.01 compared with con-trol.
그림 Fig. 5. Effe ct of MeOH Ex of S. miltiorrhiza (MESM) on Pg LPS induced IL-1β and TNF-α production in YD-38 cell and F. nucleatum (FN) induced IL-8 production in YD-38 cell. (A) Concentration of IL-1β. (B) Concentration of TNF-α. (C) Concentration of IL-8. Values are presented as mean±S.D. *p<0.05, **p<0.01 compared with control.

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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