[논문]전북지역 돼지유행성설사 바이러스 Spike 유전자 염기서열 및 계통분석

박미연; 문보미; 강수진; 이종하; 박진우; 조성우; 허철호

doi:10.7853/kjvs.2021.44.2.73

전북지역 돼지유행성설사 바이러스 Spike 유전자 염기서열 및 계통분석
Genetic sequence and phylogenetic analysis of spike genes of porcine epidemic diarrhea virus (PEDV) in Jeonbuk province 원문보기

韓國家畜衛生學會誌 = Korean journal of veterinary service, v.44 no.2, 2021년, pp.73 - 83

박미연 (전라북도 동물위생시험소 북부지소) , 문보미 (전라북도 동물위생시험소 북부지소) , 강수진 (전라북도 동물위생시험소 북부지소) , 이종하 (전라북도 동물위생시험소 북부지소) , 박진우 (전라북도 동물위생시험소 북부지소) , 조성우 (전라북도 동물위생시험소 북부지소) , 허철호 (전라북도 동물위생시험소 북부지소)

Abstract ▼ AI-Helper

Although many swine farms continuously vaccinated to sow to prevent Porcine epidemic diarrhea(PED), PED has occurred annually in swine herds in Jeonbuk province, Korea. In the present study, the small intestine and feces samples from 17 farms where severe watery diarrhea and death of newborn piglets occurred in 2019 were collected, amplified by RT-PCR and determined the complete nucleotide sequences of the spike (S) glycoprotein genes of nine Jeonbuk PEDV isolates. The spike (S) glycoprotein is an important determinant for molecular characterization and genetic relationship of PEDV. These nine complete S gene isolates were compared with other PEDV reference strains to identify the molecular diversity, phylogenetic relationships and antigenicity analysis. 9 field strains share 98.5~100% homologies with each other at the nucleotide sequence level and 97.3~100% homologies with each other at the amino acid level. The nine Jeonbuk PEDV isolates were classified into G2b group including a genetic specific signal, S-indels (insertion and deletion of S gene). In addition, comparisons the neutralizing epitopes of S gene between 9 field strains and domestic vaccine strains of Korea mutated 12-15 amino acids with SM-98-1 (G1a group) and mutated 0-3 amino acids with QIAP1401 (G2b group). Therefore, the development of G2b-based live vaccines will have to be expedited to ensure effective prevention of endemic PED in Korea. In addition, we will need to be prepared with periodic updates of preventive vaccines based on the PEDV variants for the re-emergence of a virulent strain.

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표/그림 (8)

표 Table 1. General information on PED outbreaks farms in Jeonbuk province
표 Table 2. List of primers utilized in this study
표 Table 3. The recent porcine epidemic diarrhea virus (PEDV) isolates in Jeonbuk and references strains used in this study
그림 Fig. 1. Alignment of amino N-terminal 1-234 amino acid of S proteins between the reference strains and 9 Jeonbuk strains. The dashes (−) indicate the deleted sequences. Insertions and deletions within PEDV isolates are indicated by boxes (Red box indicate the inserted amino acids compared with CV777. Blue box indicate the deleted amino acids compared with CV777).
표 Table 4. Comparison of the nucleotide and deduced amino acid sequences of S genes of PEDV references strains and PEDV isolates in Jeonbuk
표 Table 4. Continued
그림 Fig. 2. Phylogenetic analysis based on the nucleotide sequence corresponding to the full-length S gene of PEDV strains. Sequencing alignments were performed using the Clustal W program, and the phylogenetic tree was constructed from the aligned nucleotide sequences by using the neighbor- joining method. The bootstrap values were determined from 1,000 replicates of original data. the scale bars indicate the number of 0.1 inferred substitutions per site (Putative similar regions of S gene of other distantly related coronavirus, transmissible gastroenteritis virus (TGEV), was also included in this study).
그림 Fig. 3. Comparison of the antigen epitopes of field strains and reference strains. Dots indicate the amino acids are identical to those of reference strain SM-98-1. COE is in the red box, SS2 is in the blue box, SS6 is in the green box and the against 2C10 is in the orange box. The mutated amino acids within PEDV isolates are indicated by boxes and circles (Black box indicate the mutated amino acids compared with SM-98-1. Purple circle indicate the mutated amino acids compared with QIAP1401).

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