[논문]맥문동 물 추출물의 선천면역 활성과 염증억제 효과

강누리; 황덕상; 이진무; 이창훈; 장준복

doi:10.15204/jkobgy.2021.34.3.015

맥문동 물 추출물의 선천면역 활성과 염증억제 효과
The Effects of Liriopis Tuber Water Extract on Innate Immune Activation and Anti-Inflammation 원문보기

大韓韓方婦人科學會誌 = The Journal of Korean obstetrics & gynecology, v.34 no.3, 2021년, pp.15 - 28

강누리 (경희대학교 대학원 임상한의학과) , 황덕상 (경희대학교 한의과대학 부인과교실) , 이진무 (경희대학교 한의과대학 부인과교실) , 이창훈 (경희대학교 한의과대학 부인과교실) , 장준복 (경희대학교 한의과대학 부인과교실)

Abstract ▼ AI-Helper

Objectives: This study was designed to examine the anti-cancer activity by innate immunomodulating and anti-inflammatory effects of liriopis tuber water extract (LPE). Methods: Cell cytotoxicity was tested with 4T1 mouse mammary carcinoma cells, spleen cells, macrophage, and RAW264.7 cells. To investigate innate immunomodulating effects of LPE on macrophage, we measured tumor necrosis factor-alpha (TNF-α), interleukin-12 (IL-12), and interleukin-10 (IL-10). To investigate innate immunomodulating effects of LPE on RAW264.7 cell, we measured TNF-α, interleukin-6 (IL-6). In addition, TNF-α and nitric oxide (NO) induced by lipopolysaccharide (LPS) were measured after treating with LPE to observe innate immunomodulating effect of LPE on RAW264.7 cell. Also, mitogen-activated protein kinase (MAPK) and nuclear factor κB (NF-κB) were examined by western blot analysis. Results: In an in vitro cytotoxicity analysis, LPE affected tumor cell growth above specific concentration. As compared with the control group, the production of TNF-α, IL-12, and IL-10 were increased in macrophage. As compared with the control group, the production of TNF-α and IL-6 were increased in RAW 264.7 cell. The expression of TNF-α and NO induced by LPS after treating LPE was decreased. In addition, treatment of RAW 264.7 cell with LPE increased the phosphorylation levels of p-extracellular signal-regulated kinase (p-ERK), p-Jun N-terminal kinase (p-JNK), and p-p38. Conclusions: LPE might have impact on the anti-cancer effect by activation of innate immune system and inflammation control.

주제어

표/그림 (12)

그림 Fig. 1. Cytotoxicity of LPE on 4T1 mouse mammary.
그림 Fig. 2. Cytotoxicity of LPE on RAW 264.7 cell.
그림 Fig. 3. Cytotoxicity of LPE on TG-induced macrophage.
그림 Fig. 4. Production of TNF-α from TG-induced macrophage stimulated by LPE. *: p<0.05, **: p<0.01, statistically significant difference compared with media group (one-way ANOVA combined with a Dunnett T3 test).
그림 Fig. 5. Production of IL-12 from TG-induced macrophage stimulated by LPE. **: p<0.01, statistically significant difference compared with media group (one-way ANOVA combined with a Dunnett T3 test).
그림 Fig. 6. Production of IL-10 from TG-induced macrophage stimulated by LPE. **: p<0.01, statistically significant difference compared with media group (one-way ANOVA combined with a Dunnett T3 test).
그림 Fig. 7. Production of TNF-α from RAW 264.7cell stimulated by LPE. *: p<0.05, **: p<0.01, statistically significant difference compared with media group (one-way ANOVA combined with a Dunnett T3 test)
그림 Fig. 8. Production of IL-6 from RAW 264.7cell stimulated by LPE. **: p<0.01, ***: p<0.001, statistically significant difference compared with media group (one-way ANOVA combined with a Dunnett T3 test)
그림 Fig. 9. Production of TNF-α from macrophage stimulated by LPE and LPS. **: p<0.01, statistically significant difference compared with LPS group (one-way ANOVA combined with a Tukey HSD test)
그림 Fig. 10. Production of NO from macrophage stimulated by LPE and LPS. ***: p<0.001, statistically significant difference compared with LPS group (one-way ANOVA combined with a Tukey HSD test)
그림 Fig. 11. Activation of NF-κB pathway by treatment of LPE on RAW 264.7 cell.
그림 Fig. 12. Activation of MAPK pathway by treatment of LPE on RAW 264.7 cell.

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