[논문]미세먼지 흡입 과민성 천식 마우스의 간 조직에서 괭생이모자반 추출물의 항산화 효능

김효진; 김아름; 전유진; 김현정; 지영흔

doi:10.9721/kjfst.2021.53.5.535

미세먼지 흡입 과민성 천식 마우스의 간 조직에서 괭생이모자반 추출물의 항산화 효능
Antioxidant potential of Sargassum horneri extracts in the liver of mice with PM-induced asthma 원문보기

한국식품과학회지 = Korean journal of food science and technology, v.53 no.5, 2021년, pp.535 - 543

김효진 (제주대학교 식품생명공학과) , 김아름 (제주대학교 차세대융봅합과학기술협동과정) , (제주대학교 차세대융봅합과학기술협동과정) , (제주대학교 차세대융봅합과학기술협동과정) , 전유진 (제주대학교 수산생명의학과) , 김현정 (제주대학교 식품생명공학과) , 지영흔 (제주대학교 차세대융봅합과학기술협동과정)

초록
AI-Helper

본 연구에서는 호흡기를 통한 미세먼지 노출이 과민성 천식 마우스 간 조직의 산화적 스트레스에 미치는 영향을 확인하였으며, 이에 대한 괭생이모자반 추출물의 항산화 효과를 평가하였다. 세포막 지질과산화의 최종 산물인 MDA와 항산화 효소인 catalase가 미세먼지 흡입 과민성 천식 마우스의 간 조직에서 증가하였으며 SHE 병행 투여 시 감소하였다. 또한 미세먼지 흡입 과민성 천식 마우스의 간 조직에서 증가한 산화적 DNA 손상 수복효소인 OGG1은 SHE의 병행 투여 시 감소하였다. 결론적으로 괭생이모자반이 미세먼지 흡입 과민성 천식 마우스의 간 조직에서 산화적 스트레스 및 산화적 DNA 손상에 대해 항산화 효과를 나타냈다. 따라서 괭생이모자반이 미세먼지에 의한 산화적 손상을 보호하는 식품 소재로서 활용될 수 있을 것으로 기대된다.

Abstract ▼ AI-Helper

Particulate matter (PM) causes oxidative stress and can rapidly diffuse from the lung to the blood and accumulate in the liver when inhaled. Natural antioxidants can be used to protect against oxidative stress caused by PM. Sargassum horneri, a brown seaweed, possesses antioxidative activity and is a good source of functional foods. Therefore, this study investigated the antioxidant potential of S. horneri extract (SHE) in the livers of PM-induced asthmatic mice. PM inhalation triggered lipid peroxidation and oxidative stress, and SHE treatment attenuated malondialdehyde in the liver of mice with PM-induced asthma. Furthermore, SHE mitigated the increase in catalase activity. Importantly, SHE reduced the activity of 8-oxoguanine glycosylase (OGG1), a DNA repair enzyme. These results suggest that SHE has antioxidant potential for moderating PM-induced oxidative stress and DNA damage in the liver of asthmatic mice.

주제어

표/그림 (5)

그림 Fig. 1. The antioxidant effect of SHE on the level of malondialdehyde (MDA) in the liver of asthma mice model. The MDA levels in liver tissue are presented. Data represent the mean± standard deviation (SD) (n=3). **p<0.01 represent significant increase compared to vehicle and †p<0.05, ††p<0.01 represent significant decrease compared to OVA+PM.
그림 Fig. 2. The expression of antioxidant enzymes in the liver of asthma mice model. The expression of CuZnSOD (A), MnSOD (B), and catalase (C) were determined by western blot analysis. Data represent the mean±SD (n=2). *p<0.05, ***p<0.005 represent a significant increase compared to vehicle.
그림 Fig. 3. The expression of catalase (CAT) in the liver of asthma mice model determined by using immunohistochemistry (A-N). The expression of vehicle (A, H), PM only (B, I), OVA (C, J) only, OVA+PM (D, K), OVA+PM+SHE200 (E, L), OVA+PM+SHE400 (F, M), OVA+PM+Prednisone (G, N), catalase positive cells/site (O), and catalase activity (P) in the liver. Data represent the mean±SD (n=2). *p<0.05, **p<0.01, ***p<0.005 represent significant increase compared to vehicle and †p<0.05, ††p<0.01 represent significant decrease compared to OVA+PM.
그림 Fig. 4. The expression of OGG1 determined as the level of DNA damages in liver of asthma mice model. The expression of OGG1 in cytoplasm (A) andOGG1 in nucleus (B) were determined by western blot analysis. Data represent the mean±SD (n=2). *p<0.05, **p<0.01 represent significant increase compared to vehicle and ††p<0.01 represent significant decrease compared to OVA+PM.
그림 Fig. 5. The expression of of 8-oxoguanine glycosylase (OGG1) in the liver of asthma mice model determined by immunohistochemistry (A-N). The expression of vehicle (A, H), PM only (B, I), OVA only (C, J), OVA+PM (D, K), OVA+PM+SHE200 (E, L), OVA+PM+SHE400 (F, M), OVA+PM+Prednisone (G, N), and OGG1 positive cells/site (O) in the liver. Data represent the mean±SD (n=2). *p<0.05 represent significant increase compared to vehicle and ††p<0.01 represent significant decrease compared to OVA+PM.

참고문헌 (39)

Bartz RR, Sullman HB, Fu P, Welty-Wolf K, Carraway MS, MacGarvey NC, Withers CM, Sweeney TE. Piantadosi CA. Staphylococcus aureus sepsis and mitochondrial accrual of the 8-oxoguanine DNA glycosylase DNA repair enzyme in mice. Am. J. Resp. Crit. Care. 183: 226-33 (2011)

상세보기
Boo HJ, Moon HS, Lee Y, Jeon YJ, Chun JY. Appearance characteristics and antioxidant activity of jeju coast sargassum horneri according to collecting time. J. Korean Soc. Food Sci. Nutr. 49: 485-492 (2020)

상세보기
Burnett RT, Pope III CA, Ezzati M, Olives C, Lim SS, Mehta S, Shin HH, Singh G, Hurbbell B, Brauer M, Anderson HR, Smith KR, Balmes JR, Bruce NG, Kan H, Laden F, Pruss-ustum A, Turner MC, Gapstur SM, Diver WR, Cohen A. An integrated risk function for estimating the global burden of disease attributable to ambient fine particulate matter exposure. Environ. Health Persp. 122: 397-235 (2014)

상세보기
Chen Z, Wang D, Liu X, Pei W, Li J, Cao Y, Zhang J, An Y, Nie J, Tong J. Oxidative DNA damage is involved in cigarette smoke-induced lung injury in rat. Environ. Health Prev. 20: 318-324 (2015)

상세보기
Crobeddu B, Sntiago LA, Bui LC, Boland S, Squiban AB. Oxidative potential of particulate matter 2.5 as predictive indicator of cellular stress. Environ. Pollut. 230: 125-133 (2017)

상세보기
Das J, Ramani R, Suraju MO. Polyphenol compounds and PKC signaling. BBA-Gen Subjects 1860: 2107-2121 (2016)

상세보기
Ding S, Yu L, An B, Zhang G, Yu P, Wang Z. Combination effects of airborne particulate matter exposure and high-fat diet on hepatic fibrosis through regulating the ROS-endoplasmic reticulum stress-TGFβ/SMADs axis in mice. Chemosphere 199: 538-545 (2018)

상세보기
Dybdahl M, Risom L, Moller P, Autrup H, Wallin H, Vogel U, Bornholdt J, Daneshvar B, Dragsted LO, Weimann A, Poulsen HE, Loft S. DNA adduct formation and oxidative stress in colon and liver of Big Blue ® rats after dietary exposure to diesel particles. Carcinogenesis 24: 1759-1766 (2003)

상세보기
Fernando IPS, Dias MKHM, Madusanka DMD, Han EJ, Kim MJ, Jeon YJ, Lee K, Cheong SH, Han YS, Park SR, Ahn G. Human keratinocyte UVB-protective effects of a low molecular weight fucoidan from sargassum horneri purified by step gradient ethanol precipitation. Antioxidants 9: 1-15 (2020)
Fernando IPS, Jayawardena TU, Sanjeewa KKA, Wang L, Jeon YJ, Lee WW. Anti-inflammatory potential of alginic acid from sargassum horneri against urban aerosol-induced inflammatory responses in keratinocytes and macrophages. Ecotox. Environ. Safe. 160: 24-31 (2018)

상세보기
Herath KHINM, Cho J, Kim A, Kim HS, Han EJ, Kim HJ, Kim MS, Ahn G, Jeon YJ, Jee Y. Differential modulation of immune response and cytokine profiles of sargassum horneri ethanol extract in murine spleen with or without Concanavalin A stimulation. Biomed. Pharmacother. 110: 930-942 (2019)

상세보기
Herath KHINN, Kim HJ, Jang JH, Kim HS, Kim HJ, Jeon YJ, Jee Y. Mojabanchromanol isolated from sargassum horneri attenuates particulate matter induced inflammatory responses via suppressing TLR2/4/7-MAPK signaling in MLE-12 cells. Mar. Drugs 18: 1-14 (2020a)
Heath KHINM, Kim HJ, Lee JH, Je JG, Yu HS, Jeon YJ, Kim HJ, Jee Y. Sargassum horneri (Turner) C. Agardh containing polyphenols attenuates particulate matter-induced inflammatory response by blocking TLR-mediated MYD88-dependent MAPK signaling pathway in MLE-12 cells. J. Ethnopharmacol. 4: 113340 (2020b)
Herath KHINM, Kin HJ, Mihindukulassoriya SP, Kim A, Kim HJ, Jeon YJ, Jee Y. Sargassum horneri extract containing mojabanchromanol attenuates the particulate matter exacerbated allergic asthma through reduction of Th2 and Th17 response in mice. Environ. Pollut. 265: 114094 (2020c)

상세보기
Josephine A, Nithya K, Amudha G, veena CK, Preetha SP, Varalakshmi P. Role of sulphated polysaccharides from sargassum wightii in Cyclosporine A-induced oxidative liver injury in rats. BMC Pharmacol. Toxico. 8: 1-9 (2008)

상세보기
Jun JY, Lee SY, Kim BM, Jeong IH. Effect of lactic acid extracts of sargassum horneri on bone formation in female sparge-dawley rats. Korean J Fish Aquat Sci 44: 25-30 (2011)

원문보기 상세보기
Kim YD, Mahinda S, Koh KS, Jeon YJ, Kim SH. Reactive oxygen species scavenging activity of jeju native citrus peel during maturation. J. Korean Soc. Food Sci. Nutr. 38: 462-469 (2009)

원문보기 상세보기
Kim DS, Sung NY, Han IJ, Lee BS, Park SY, Nho EY, Eom J, Kim G, Kim KA. Splenocyte-mediated immune enhancing activity of sargassum horneri extracts. J. Nutr. Health 52: 515-528 (2019)

원문보기 상세보기
Kim DS, Sung NY, Park SY, Kim G, Ji E, Yoo JG, Seo IR, Han IJ, Cho YB, Kim KA. Immunomodulating activity of sargassum horneri extract in Raw264.7 macrophages. J. Nutr. Health 51: 507-514 (2018)

원문보기 상세보기
Knudsen KB, Northeved H, Kumar EKP, Permin A, Gjetting T, Andresen TL, Larsen S, Wegener KM, Lykkesfeldt J, Jantzen K, Loft S, Moller P, Roursgaard M. In vivo toxicity of cationic micelles and liposomes. Nanomed-Nanotechnol 11: 467-477 (2015)

상세보기
Lee JH, Kim HJ, Jee Y, Jeon YJ, Kim HJ. Antioxidant potential of sargassum horneri extract against urban particulate matter-induced oxidation. Food Sci. Biotechnol. 29: 855-865 (2020)

상세보기
Lemasters JJ, Jaeschke H. Oxidative stress and inflammation in the liver. The liver: Biology and pathobiology, sixth edition. Wiley, Hoboken, NJ, USA, pp.714-727 (2020)
Lim S, Kwon M, Jeong EJ, Shin T, Oh CW, Choi JS, Kim HR. Meroterpenoid-rich fraction of the ethanolic extract from sargassum serratifolium suppressed oxidative stress induced by tertbutyl hydroperoxide in HepG2 cells. Mar. Drugs 16: 1-13 (2018)

상세보기
Luceri C, Bigagli E, Femia AP, Caderni G, Giovannelli L, Lodovici M. Aging related changes in circulating reactive oxygen species (ROS) and protein carbonyls are indicative of liver oxidative injury. Toxicol. Rep. 5: 141-145 (2018)

상세보기
Moon KM, Park SH, Heo MS. Phylogenetic diversity and community structure of microbiome isolated from sargassum horneri off the Jeju island coast. J. Life Sci. 28: 1179-1185 (2018)

원문보기 상세보기
Panieri E, Santoro MM. ROS homeostasis and metabolism: a dangerous liason in cancer cells. Cell Death Dis 7: e2253 (2016)

상세보기
Pardo M, Shuster-Meiseles T, Levin-Zaidman S, Rudich A, Rudich Y. Low cytotoxicity of inorganic nanotubes and fullerene-like nanostructures in human bronchial epithelial cells: Relation to inflammatory gene induction and antioxidant response. Environ. Sci. Technol. 48: 3457-3466 (2014)

상세보기
Pardo M, Xu F, Qiu X, Zhu T, Rudich Y. Seasonal variations in fine particle composition from Beijing prompt oxidative stress response in mouse lung and liver. Sci. Total Environ. 626: 147-155 (2018)

상세보기
Park SK, Kang JY, Kim JM, Yoo SK, Han HJ, Shin EJ, Heo HJ. Cellular protective effect of ecklonia cava extract on ultra-fine dust (PM2.5)-induced cytotoxicity. Korean J. Food Sci. Technol. 51: 503-508 (2019)

원문보기 상세보기
Pedersen M, Andersen ZJ, Stafoggia M, Weinmayr G, Galassi C, Sorensen M, Eriksen KT, Tjonneland A, Loft S, Jaesch A, Nagel G, Concin H, Tsai MY, Grioni S, Marcon A, Krogh V, Ricceri F, Sacerdote C, Ranzi A, Sokhi R, Vermeulen R, Hoogh KD, Wang M, Beelen R, Vineis P, Brunekreef B, Hoek G, Raaschou-Nielsen O. Ambient air pollution and primary liver cancer incidence in four European cohorts within the ESCAPE project. Environ. Res. 15: 226-233 (2017)
Qiu YN, Wang GH, Zhou F, Hao JJ, Tian L, Guan LF, Geng XK, Ding YC, Wu HW, Zhang KZ. PM2.5 induces liver fibrosis via triggering ROS-mediated mitophagy. Ecotoxicol. Environ. Saf. 167: 178-187 (2019)

상세보기
Quijano C, Trujillo M, Castro L, Trostchnsky A. Interplay between oxidant species and energy metabolism. Redox Biol. 8: 28-42 (2016)

상세보기
Ribeiro JDP, Quijano MFC, Ferreiro JD, Gioda A, Velez BJ, Monserrat JM, Gioda CR. Aqueous particulate matter (PM2.5) from Brazil alters antioxidant profile responses and causes oxidative stress. Atmos. Pollut. Res. 11: 511-519 (2020)

상세보기
Riis B, Risom L, Loft S, Poulsen HE. OGG1 mRNA expression and incision activity in rats are higher in foetal tissue than in adult liver tissue while 8-oxo2'-deoxyguanosine levels are unchanged. DNA Repair 1: 709-717 (2002)

상세보기
Rio DD, Stewart AJ, Pellegrini N. Areview of recent studies on malondialdehyde as toxic molecule and biological marker of oxidative stress. Nutr. Metab. Cardiovasc. Dis. 15: 316-328 (2005)

상세보기
Scheffler K, Rachek L, You P, Rowe AD, Wang W, Ku snierczyk A, Kittelsen L, Bjoras M, Eide L. 8-Oxoguanine DNA glycosylase (Ogg1) controls hepatic gluconeogenesis. DNA Repair 61: 56-62 (2018)

상세보기
Shipeng Y, Woo HC, Choi JH, Park YB, Chun BS, Measurement of antioxidant activities and phenolic and flavonoid contents of the brown seaweed sargassum horneri: comparison of supercritical CO 2 and various solvent extractions. Korean J Fish Aquat Sci 18: 123-130 (2015)
Ueta E, Tadokoro Y, Yamamoto T, Yamane C, Suzuki E, Nanba E, Otsuka Y, Kurata T. The effect of cigarette smoke exposure and ascorbic acid intake on gene expression of antioxidant enzymes and other related enzymes in the livers and lungs of shionogi rats with osteogenic disorders. Toxicol. Sci. 73: 339-347 (2003)

상세보기
Ya P, Xu H, Ma Y, Fang M, Yan X, Zou J, Li F. Liver injury induced in Balb/c mice by PM2.5 exposure and its alleviation by compound essential oils. Biomed. Pharmacother. 105: 590-598 (2018)

상세보기

저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

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용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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