[논문]사삼생맥산 추출물이 Balb/c mice의 면역활성에 미치는 효과

박채현; 강재희; 이현

doi:10.15188/kjopp.2022.12.36.6.235

사삼생맥산 추출물이 Balb/c mice의 면역활성에 미치는 효과
Effect of Sasamsaengmaek-san Extract on Immunity Activity in Balb/c Mice 원문보기

동의생리병리학회지 = Journal of physiology & pathology in Korean Medicine, v.36 no.6, 2022년, pp.235 - 241

박채현 (대전대학교 한의과대학 침구학교실) , 강재희 (대전대학교 한의과대학 침구학교실) , 이현 (대전대학교 한의과대학 침구학교실)

Abstract ▼ AI-Helper

The purpose of this study was to investigate the immune activation effect of Sasamsaengmaek-san (SSSMS) consisted of a mixture of Adenophora triphylla var. japonica, Liriope platyphylla, Panax ginseng C. A. Meyer and Schisandra chinensis. in Balb/c mice. Measuring alanine aminotransferase (ALT) and aspartic acid transaminase (AST) levels in Balb/c mice was performed to analyze the cytotoxicity. Cytokines (IFN-γ, IL-2, IL-12) which regulate the immune activation in Balb/c mice were measured by enzyme-linked immunosorbent assay (ELISA). Activated T lymphocytes in peripheral blood mononuclear cell (PBMC), spleen, lymph nodes were analyzed by flow cytometry using percentages. All tests were compared with red ginseng 100 ㎍/mL (RG 100), which is the most used for immune activity. As a result, cytokine activity was significantly increased at SSSMS 300 group. Activated T lymphocytes in PBMC, spleen, lymph nodes were significantly increased at SSSMS 300 group. These results suggest that there is a possibility of SSSMS activating an immune system by activating the cytokines, and it is confirmed that SSSMS also effective for generation and differentiation of T, B lymphocytes which activate the immune response.

주제어

표/그림 (8)

그림 Fig. 1. Effects of extracts of Sasamsaengmaek-san on Asparate aminotransferase (AST), Alanine aminotransferase (ALT) levels in mice. Mice were treated with Red Ginseng components (RG 100) once a day and extract of Sasamsaengmaek-san for 2 weeks. Negative control : Not treated Balb/c mouse group. RG100 : Balb/c mouse group treated with RG 100 mg/kg once a day for 2 weeks. SSSMS 150 : Balb/c mouse group treated with SSSMS 150 mg/kg once a day for 2 weeks. SSSMS 300 : Balb/c mouse group treated with SSSMS 300 mg/kg once a day for 2 weeks. The results are expressed the mean ± S.E.M. (N=6). Comparisons between control and test groups were analyzed using ANOVA.
표 Table 1. Composition of Herbal Complex Extract
그림 Fig. 2. Effects of extracts of Sasamsaengmaek-san on IL-2 cytokine expressions in mouse splenocyte culture. After mice were treated with Red Ginseng components (RG 100) once a day and extract of Sasamsaengmaek-san for 2 weeks, blood was collected and serum was obtained by 20.000 rpm centrifugation. IL-2 cytokine gene expressions were analyzed by enzyme-linked immuno-sorbent assay. The results are expressed the mean ± S.E.M. (N=12). Comparisons between control and test groups were analyzed using ANOVA. **p < 0.05 vs normal by Tukey HSD.
그림 Fig. 3. Effects of extracts of Sasamsaengmaek-san on IL-12 Cytokine Expressions in mouse spleen.
그림 Fig. 4. Effects of extracts of Sasamsaengmaek-san on IFN-γ Cytokine Expressions in mouse spleen. Comparisons between control and test groups were analyzed using Kruskal Wallis test, post hoc test was performed by Mann-Whitney U test. Bonferroni adjustment was made with statistical significance defined at P less than 0.008. ***p < 0.001 vs normal by Mann-Whitney U test.
그림 Fig. 5. Effects of extracts of Sasamsaengmaek-san T cell ratio in peripheral blood mononuclear cells of mice. Peripheral blood mononuclear cells were isolated following 2 weeks administration of extract of Sasamsaengmaek-san.
그림 Fig. 6. Effects of extracts of Sasamsaengmaek-san on the T cell ratio in spleen cells of mice. Spleen cells were isolated following 2 weeks administration of extract of Sasamsaengmaek-san.
그림 Fig. 7. Effects of extracts of Sasamsaengmaek-san on the T cell ratio in Draining lymph nodes (DLN) cells of mice. Draining lymph nodes (DNL) cells were isolated following 2 weeks administration of extract of Sasamsaengmaek-san.

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