[논문]Comparative mitogenomics of Pleurotus ostreatus Gonji7ho and its cap color mutant

Oh, Min-Ji; Na, Kyeong Sook; Jung, Hwa Jin; Lee, Young Kuk; Ryu, Jae-San

doi:10.14480/jm.2022.20.2.43

Comparative mitogenomics of Pleurotus ostreatus Gonji7ho and its cap color mutant 원문보기

Journal of mushrooms = 한국버섯학회지, v.20 no.2, 2022년, pp.43 - 49

Oh, Min-Ji (Mushroom Research Division, National Institute of Horticultural & Herbal Science, RDA) , Na, Kyeong Sook (Department of Mushroom Science, Korea National College of Agriculture and Fisheries) , Jung, Hwa Jin (Department of Mushroom Science, Korea National College of Agriculture and Fisheries) , Lee, Young Kuk (Department of Mushroom Science, Korea National College of Agriculture and Fisheries) , Ryu, Jae-San (Department of Mushroom Science, Korea National College of Agriculture and Fisheries)

Abstract ▼ AI-Helper

Pleurotus ostreatus is a globally cultivated mushroom crop. Cap color is a quality factor in P. ostreatus. However, cap color can spontaneously mutate, degrading the quality of the mushroom on the market. Early detection and removal of mutant strains is the best way to maintain the commercial value of the crop. To detect the cap color mutant Gonji7ho, molecular markers were developed based on insertion/deletions (InDels) derived from the comparison of mitogenomes of Gonji7ho and Gonji7hoM mushrooms. Sequencing, assembly, and comparative analysis of the two mitogenomes revealed genome sizes of 73,212 bp and 72,576 bp with 61 and 57 genes or open reading frames (ORFs) in P. ostreatus Gonji7ho and Gonji7hoM, respectively. Fourteen core protein-encoding genes, two rRNA, and 24 tRNA with some OFRs were predicted. Of the 61 genes or OFRs in the wild type, dpo, rpo, and two orf139 were missing (or remnant) in the mutant strain. Molecular markers were developed based on the sequence variations (InDels) between the two mitogenomes. Six polymorphic molecular markers could detect the mutated mitochondria by PCR. These results provide basic knowledge of the mitogenomes of wild-type and mutant P. ostreatus, and can be applied to discriminate mutated mitochondria.

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표/그림 (5)

그림 Fig. 1. Phenotypic characteristics of the P. ostreatus cultivar Gonji7ho (right) and its cap color mutant Gonji7hoM (left).
표 Table 1. List of primers for the discrimination of mutated mitochondria
표 Table 2. Morphological characteristics of Gonji7ho and its cap color mutant Gonji7hoM
그림 Fig. 2. Physical map of the mtDNA of P. ostreatus cultivar Gonji7ho (right) and its cap color mutant Gonji7hoM (left). The mitogenomic map was drawn using OGDraw online (https://chlorobox.mpimp-golm. mpg.de/OGDraw.html) with basic parameters. Genes are shown in the colored box and grouped by function. The protein-coding genes are shown outside and inside the circle with colored boxes. The direction of each gene is indicated by arrows. The inner circle in gray shows the GC ratio (%).
그림 Fig. 3. DNA fragments amplified by PCR using the CAP 1-6 primer set specific for P. ostreatus cultivar Gonji7ho and its cap color mutant Gonji7hoM. M, 100-bp plus DNA ladder (Bioneer, Daejeon, Korea); G, P. ostreatus Gonji7ho; G7M, P. ostreatus Gonji7hoM.

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표제어: PCR

동의어: Packet Collision Rate

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