[논문]넙치(Paralichthys olivaceus) 알로부터 등전점 용해/침전공정에 의해 회수한 분리단백질의 식품특성

강상인; 권인상; 김형준; 윤인성; 최유리; 이정석; 김진수; 허민수

doi:10.5657/kfas.2023.0162

넙치(Paralichthys olivaceus) 알로부터 등전점 용해/침전공정에 의해 회수한 분리단백질의 식품특성
Food Characteristics of Protein Isolates Recovered from Olive Flounder Paralichthys olivaceus Roe by Isoelectric Solubilization and Precipitation Process 원문보기

한국수산과학회지 = Korean journal of fisheries and aquatic sciences, v.56 no.2, 2023년, pp.162 - 173

강상인 (경상국립대학교 해양식품공학과) , 권인상 (경상국립대학교 식품영양학과) , 김형준 (식품의약품안전처) , 윤인성 (경상국립대학교 해양식품공학과) , 최유리 (경상국립대학교 해양식품공학과) , 이정석 (경상국립대학교 해양식품공학과) , 김진수 (경상국립대학교 해양식품공학과) , 허민수 (경상국립대학교 식품영양학과)

Abstract ▼ AI-Helper

Four roe protein isolates (RPIs) from olive flounder Paralichthys olivaceus roes (OFR) were recovered by isoelectric solubilization (pH 11 and 12) and precipitation (pH 4.5 and 5.5) and investigated for their food characteristics. RPIs contained 4.5-9.6% moisture, 64.1-69.5% protein, 16.1-19.8% lipid, and 1.0-3.9% ash. The protein yields of RPIs ranged from 50.1 to 56.8%, which was significantly different depending on the recovery conditions. A difference was observed in the SDS-PAGE protein band (25-100 kDa) between the alkaline solubilization at pH 11 (RPI-1 and 2) and pH 12 (RPI-3 and 4). The major amino acids of RPIs were Leu, Lys, Asp, Glu and Ala and major mineral components were sulfur, sodium, phosphorus, and magnesium, which were significantly different from OFR (P<0.05). Additionally, the lead and cadmium content was below the chemical hazard standard of the Korean food standards code. The Hunter color and whiteness of RPIs also showed significant differences according to the treatment conditions of the ISP process (P<0.05). This suggests that protein isolates recovered from olive flounder roes have high potential as nutritional supplements.

주제어

표/그림 (7)

그림 Fig. 1. Production process of roe protein isolates (RPIs) from olive flounder Paralichthys olivaceus by isoelectric solubilization and precipitation process.
표 Table 1. Yield and proximate composition (g/100 g sample) of roe protein isolates recovered from olive flounder Paralichthys olivaceus by isoelectric solubilization and precipitation process
그림 Fig. 2. SDS-PAGE patterns of roe protein isolates recovered from olive flounder Paralichthys olivaceus using isoelectric solubilization and precipitation process. Lane 1, Protein maker; Lane 2, Olive flounder roe; Lane 3-4, RPI-1 and RPI-2, Roe protein isolate adjusting at pH 4.5 and 5.5, respectively, after alkaline solubilization at pH 11; Lane 5-6, RPI-3 and RPI-4 roe protein isolate adjusting at pH 4.5 and 5.5, respectively, after alkaline solubilization at pH 12.
표 Table 2. Amino acid composition (g/100 g protein) of roe protein isolates recovered from olive flounder Paralichthys olivaceus using isoelectric solubilization and precipitation process
표 Table 3. Mineral contents of roe protein isolates recovered from olive flounder Paralichthys olivaceus by isoelectric solubilization and precipitation process
표 Table 4. Heavy metal contents of roe protein isolates recovered from olive flounder Paralichthys olivaceus by isoelectric solubilization and precipitation process
표 Table 5. Hunter's colour values and whiteness of roe protein isolates recovered from olive flounder Paralichthys olivaceus by isoelectric solubilization and precipitation process

AI 본문요약
AI-Helper

제안 방법

OFR로부터 ISP 공정을 통해 회수한 RPIs의 색차는 명도 (lightness, L), 적색도(redness, a), 갈색도(brownness, b)에 대해 직시색차계(ZE-2000; Nippon Denshoku Indusries Co., Tokyo, Japan)를 사용하여 측정하였으며, 색차를 측정 전에 보정용 white plate (L=96.92, a= -0.38, b=0.64)를 이용하여 보정하였다. 또한 백색도는 다음 식으로부터 구하였다.
RPIs의 무기질 및 중속금 함량은 유도결합 플라즈마 분석기 (inductively coupled plasma optical emission spectrophotometer, Optima 4300 DV; Perkin Elmer, Shelton, CT, USA)로 분석하였다. 즉, 테프론 분해용기에 1 g의 시료를 담아 10 mL의 중금속 분석용 고순도 70% (v/v) 질산을 가해 시료가 완전히 분해될 때까지 hot plate에서 가열하였다.

대상 데이터

넙치 알(olive flounder Paralichthys olivaceus roe, OFR)는 2020년 4월 통영시 소재 수산시장에서 살아있는 넙치를 구입하여, 실험실로 운반한 후, 알을 적출하여 실험에 사용하였다. 적출한 OFR은 흐르는 물에 간단히 수세한 후, 파우치형 티백 (tea bag, polyethylene polyprophylene, 16×14.

데이터처리

데이터는 SPSS 12.0 K (SPSS Inc., Chicago, IL, USA) 통계프로그램을 이용하여 ANOVA test를 통해 분산분석을 실시하고, Duncan의 다중위 검정법으로 최소유의차검정(P<0.05)을 실시하였다.

성능/효과

ISP 공정을 통해 최종적으로 회수한 분리단백질로서 RPIs는 카드뮴 0.1–0.2 mg/kg, 크롬 0.0–0.1 mg/kg, 구리 0.4–2.0 mg/kg, 납 0.1–0.3 mg/kg 그리고 니켈이 0.0–0.3 mg/kg 수준을 나타내었다
OFR로부터 알칼리 용해과정(pH 11 및 12)에 이어서 산 침전과정(pH 4.5 및 5.5)로부터 회수한 분리단백질은 각각 RPI-1 (pH 11/4.5), RPI-2 (pH 11/5.5), RPI-3 (pH 12/4.5) 그리고 RPI-4 (pH 12/5.5)로 표시하고, 이들의 겉보기 수율은 원료 100 g에 대해 pH 4.5의 산 침전공정을 거친 RPI-1과 3이 13.2 g으로 pH 5.5의 산 침전공정을 거친 RPI-2와 4 (12.6 및 12.9 g)에 비하여 상대적으로 높은 수율을 나타내었으며, 이때의 단백질 수율은 8.1–9.2 g/100 g수준으로 OFR 단백질의 50.0–56.8%가 회수되었다.
7)이 유의적으로 다른 RPIs에 비해 밝았다. 또한 RPI-1과 3이 RPI-2와 4에 비해 유의적으로 높은 수치를 나타냄으로서 알칼리 용해과정 후에 이어지는 pH 4.5의 산 침전과정이 pH 5.5에서 보다 명도에 영향을 주는 색소의 침착이 덜 한 것으로 추정되었다.
또한 구리의 경우, RPI-3 (pH 12/4.5)이 2.0 mg/kg으로 가장 높은 함량을 나타내었으며, 다음으로 RPI-1 (1.1 mg/kg), RPI-4 (0.9 mg/kg) 그리고 RPI-2 (0.4 mg/kg)의 순으로 각각의 처리조건에 따른 유의성이 인정되었다(P<0.05)
이상의 결과와 연구보고에 비추어, OFR에서 ISP 공정을 통해 회수한 RPIs는 단백질의 회수율 측면에서 pH 12에서의 알칼리 용해과정(alkaline solubilization)을 거친 다음, pH 4.5에서 산 침전과정(acid precipitation)을 행하는 조건이 유리할 것으로 판단되었다.
특히 카드뮴, 구리 및 납의 경우, ISP 공정의 처리조건에 따른 유의적인 차이가 있었으며(P<0.05), RPI-1과 RPI-3의 중금속 함량이 RPI-2 및 RPI-4에 비하여 원료 알에 대해 보다 많은 양을 축적한 것으로 확인 되었다
한편 ISP 공정의 최종 결과물로써, 분리단백질인 RPIs의 주요 무기질성분은 황(S, 435.9–639.9 mg/100 g), 소듐(Na, 154.0–304.2 mg/100 g), 인(P, 24.5–124.5 mg/100 g) 그리고 마그네슘(Mg, 38.3–65.6 mg/100 g) 순이었으며, OFR과 주요 무기질성분 및 함량에 있어 유의적인 차이를 나타내었다(P<0.05).

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저자의 다른 논문 :

표제어: PCR

동의어: Packet Collision Rate

용어 설명 출처 목록 (6)

용어 설명: PCR은 세균 특이성이 있는 primer를 이용하여 적은 수의 세균이 있을지라도 쉽게 검출할 수 있는 유용한 방법이며, 이를 이용하여 구강 내 치면세균막이나 타액에서 직접 세균을 검출할 수 있게 되었다[8].

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