자가수분한 한국자생춘란의 유묘를 RAPD로 이용하여 무늬를 중심으로 한 표현형과 유전적 다형성을 조사하였다. 잎의 무늬형태는 정상적인형, 녹중투형, 얼룩무늬형, 녹황색형과 황록색무늬형의 5가지로 나눌 수 있었다. 정상적인 형은 26개체(56%), 녹중투형은 9개체(19%), 얼룩무늬형은 8개체(17%), 녹황색 서(曙)는 2개체(4%), 황록색 서(曙)는 1개체(2%) 로 조사되었다. 10종류의 primer를 사용하여 RAPD 방법으로 46개체의 춘란유묘에 있어서 다형성을 검정한 결과 총 137개의 band 중에 polymorphic band 134개와 3개의 monomorphic한 band를 얻을 수 있었다. 증폭된 DNA band의 크기는 300～2,500 bp사이에 존재하였고, 이들의 전체적인 유사도는 0.590～0.874의 범위로 나타났다. 유연관계 분석 결과 자가수정한 한국춘란 유묘들은 6개의 소그룹으로 나눌 수 있었다. Ⅰ그룹은 1, 2, 3, 4, 6, 7번 등 6개체(13%) 였고 Ⅱ그룹은 5, 8, 9, 10, 11, 12번의 6개체(13%), Ⅲ 그룹은 14, 15, 16, 17, 18, 20, 21, 22, 23번 등 9개체(19.6%), Ⅳ 그룹은 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36번 등 13개체(28.3%), Ⅴ 그룹은 37, 38, 39, 40번 등 4개체(8.7%), Ⅵ 그룹은 41, 42, 43, 44, 45, 46번등 6개체(13%) 로 그룹지을 수 있었고, 13번과 19번은 무늬가 없는 단엽형이었고 유연관계에 있어서도 어느 그룹에도 속하지 않았다.
자가수분한 한국자생춘란의 유묘를 RAPD로 이용하여 무늬를 중심으로 한 표현형과 유전적 다형성을 조사하였다. 잎의 무늬형태는 정상적인형, 녹중투형, 얼룩무늬형, 녹황색형과 황록색무늬형의 5가지로 나눌 수 있었다. 정상적인 형은 26개체(56%), 녹중투형은 9개체(19%), 얼룩무늬형은 8개체(17%), 녹황색 서(曙)는 2개체(4%), 황록색 서(曙)는 1개체(2%) 로 조사되었다. 10종류의 primer를 사용하여 RAPD 방법으로 46개체의 춘란유묘에 있어서 다형성을 검정한 결과 총 137개의 band 중에 polymorphic band 134개와 3개의 monomorphic한 band를 얻을 수 있었다. 증폭된 DNA band의 크기는 300～2,500 bp사이에 존재하였고, 이들의 전체적인 유사도는 0.590～0.874의 범위로 나타났다. 유연관계 분석 결과 자가수정한 한국춘란 유묘들은 6개의 소그룹으로 나눌 수 있었다. Ⅰ그룹은 1, 2, 3, 4, 6, 7번 등 6개체(13%) 였고 Ⅱ그룹은 5, 8, 9, 10, 11, 12번의 6개체(13%), Ⅲ 그룹은 14, 15, 16, 17, 18, 20, 21, 22, 23번 등 9개체(19.6%), Ⅳ 그룹은 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36번 등 13개체(28.3%), Ⅴ 그룹은 37, 38, 39, 40번 등 4개체(8.7%), Ⅵ 그룹은 41, 42, 43, 44, 45, 46번등 6개체(13%) 로 그룹지을 수 있었고, 13번과 19번은 무늬가 없는 단엽형이었고 유연관계에 있어서도 어느 그룹에도 속하지 않았다.
RAPD analysis of leaf patterns from phenotypic expression and genetic diversification of self-pollinated seedlings of the Korean native Cymbidium goeringii was investigated. The leaf color patterns could be classified into five categories; green, green flecked mediovariegata, variegata, greenish yel...
RAPD analysis of leaf patterns from phenotypic expression and genetic diversification of self-pollinated seedlings of the Korean native Cymbidium goeringii was investigated. The leaf color patterns could be classified into five categories; green, green flecked mediovariegata, variegata, greenish yellow and yellowish green misted variegata. According to the visual observations, 26 plant leaves were green (56%), 9 were green mediovariegata (19%), 8 were flecked variegata (17%), 2 were greenish yellow (4%), and 1 was yellowish green misted variegata (2%). Of the self-pollinated Cymbidium seedlings, an average of 46 plants had leaf petals with a width that ranged from 3.9 to 13.2 cm, while two plants, expressed extremely dwarfed leaf widths of 4.4 cm and 3.9 cm respectively. The polymorphism of DNA bands in 46 Cymbidium seedlings through manipulation of ten types of primers using RAPD methodologies showed 137 bands, consisted of 134 polymorphic and 3 monomorphic bands. The size of the amplified DNA band ranged from 300 to 2,500 bp, and overall similarity value ranged from 0.590 to 0.874. The results of analysis of collected data through the method of UPGMA clustering can be categorized into six subgroups. Group Ⅰ covered six plants (13%), Group Ⅱ covered six plants (13%), Group Ⅲ covered nine plants (19.6), Group Ⅳ covered 13 plants (28.3%), Group Ⅴ covered four plants (8.7%), and Group Ⅵ covered six plants (13%). The two plants, numbers 13 and 19, were patternless dwarf types and even in the case of being found to have a genetic similarity relationship, they did not fit any of the above six categories.
RAPD analysis of leaf patterns from phenotypic expression and genetic diversification of self-pollinated seedlings of the Korean native Cymbidium goeringii was investigated. The leaf color patterns could be classified into five categories; green, green flecked mediovariegata, variegata, greenish yellow and yellowish green misted variegata. According to the visual observations, 26 plant leaves were green (56%), 9 were green mediovariegata (19%), 8 were flecked variegata (17%), 2 were greenish yellow (4%), and 1 was yellowish green misted variegata (2%). Of the self-pollinated Cymbidium seedlings, an average of 46 plants had leaf petals with a width that ranged from 3.9 to 13.2 cm, while two plants, expressed extremely dwarfed leaf widths of 4.4 cm and 3.9 cm respectively. The polymorphism of DNA bands in 46 Cymbidium seedlings through manipulation of ten types of primers using RAPD methodologies showed 137 bands, consisted of 134 polymorphic and 3 monomorphic bands. The size of the amplified DNA band ranged from 300 to 2,500 bp, and overall similarity value ranged from 0.590 to 0.874. The results of analysis of collected data through the method of UPGMA clustering can be categorized into six subgroups. Group Ⅰ covered six plants (13%), Group Ⅱ covered six plants (13%), Group Ⅲ covered nine plants (19.6), Group Ⅳ covered 13 plants (28.3%), Group Ⅴ covered four plants (8.7%), and Group Ⅵ covered six plants (13%). The two plants, numbers 13 and 19, were patternless dwarf types and even in the case of being found to have a genetic similarity relationship, they did not fit any of the above six categories.
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