Biochemical genetic variations and relationships for populations of Tribolodon brandti and Tribolodon hakonensis in the Suifen River, China
中國水産科學 = Journal of fishery sciences of China,
v.12 no.6,
2005년, pp.688 - 693
MA, Bo
(Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences)
,
CHEN, Jinping
(Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences)
,
DONG, Chongzhi
(Heilongjiang River Fisheries Research Institute, Chinese Academy of Fishery Sciences)
Triboloclon brandti and Tribolodon hakonensis are especially economic fishes. The adults migrate into freshwater rivers, the Suifen River and the Tureen River, from the sea of Japan for spawning. In order to discuss the hereditary constitution and variation of the two populations and investigate the...
Triboloclon brandti and Tribolodon hakonensis are especially economic fishes. The adults migrate into freshwater rivers, the Suifen River and the Tureen River, from the sea of Japan for spawning. In order to discuss the hereditary constitution and variation of the two populations and investigate the character of genetic differentiation, genetic relationship and classified position, the starch gel electrophoresis was used to analyze the isoenzymes in the Suifen River breeding populations. The results demonstrated that twelve isoenzymes (LDH, MDH, ME, IDH, α-GPD, ADH, SDH, GPI, CAT, AAT, EST and SOD) were coded by 27 gene loci. Six loci (Ldh-1 , a-Gpd-1 , Sdh-1 , Gpi-1 , Aat-1 and Est ) in T. brandti population and five loci ( Ldh-1 , a-Gpd-1, Sdh-1, Aat-1 and Est ) in T. hakonensis population were found polymorphic, and the mean proportions of polymorphic loci in the two populations were 22.22 % and 18.52 % respectively, and average heterozygosity (H) of the two species of fishes were 0.067 0 and 0. 069 5. Allele frequencies of Ldh-1^100, a-Gpd-1^-100 Sdh-1^-100, Aat-1^-100 and Est^120 in T. brandti population was significantly higher than T. hakonensis', and allele frequencies of Ldh-1 o a-Gpd-1 - 147 Sdh-1 ^- 144 Aat-1^- 116 and Est^100 in T. hakonensis population was striking higher than those in T. brandti. In addition, the two species of fish possess their own prevalent allele respectively. The observational value of heterozygosis(Ho) of Ldh-1 , α-Gpd-1 , Sdh-1 and Aat-1 was 0, and their deviation index (d value) of heterozygosis was-1.000 in the two populations. Ho of Est in T. brandti population was 0.900, and d value was 0.818. Remarked deviations from Hardy-Weinberg equilibrium were observed at the loci of Ldh-1 , a-Gpd-1 , Sdh-1 and Aat-1 in the two species of fishes except Gpi-1 in T. brandti and Est in T. hakonensis. Sig- nificant heterozygote absent was observed with Ldh-1 , α-Gpd-1, Sdh-1 and Aat-1 , as well as the homozy- gote excess with Est in T. brandti and T. hakonensis. The genetic distance and genetic similarity between the two breeding fishes was 0.369 3 and 0.691 2. The genetic diversity levels of the two fishes were up to species level.
Triboloclon brandti and Tribolodon hakonensis are especially economic fishes. The adults migrate into freshwater rivers, the Suifen River and the Tureen River, from the sea of Japan for spawning. In order to discuss the hereditary constitution and variation of the two populations and investigate the character of genetic differentiation, genetic relationship and classified position, the starch gel electrophoresis was used to analyze the isoenzymes in the Suifen River breeding populations. The results demonstrated that twelve isoenzymes (LDH, MDH, ME, IDH, α-GPD, ADH, SDH, GPI, CAT, AAT, EST and SOD) were coded by 27 gene loci. Six loci (Ldh-1 , a-Gpd-1 , Sdh-1 , Gpi-1 , Aat-1 and Est ) in T. brandti population and five loci ( Ldh-1 , a-Gpd-1, Sdh-1, Aat-1 and Est ) in T. hakonensis population were found polymorphic, and the mean proportions of polymorphic loci in the two populations were 22.22 % and 18.52 % respectively, and average heterozygosity (H) of the two species of fishes were 0.067 0 and 0. 069 5. Allele frequencies of Ldh-1^100, a-Gpd-1^-100 Sdh-1^-100, Aat-1^-100 and Est^120 in T. brandti population was significantly higher than T. hakonensis', and allele frequencies of Ldh-1 o a-Gpd-1 - 147 Sdh-1 ^- 144 Aat-1^- 116 and Est^100 in T. hakonensis population was striking higher than those in T. brandti. In addition, the two species of fish possess their own prevalent allele respectively. The observational value of heterozygosis(Ho) of Ldh-1 , α-Gpd-1 , Sdh-1 and Aat-1 was 0, and their deviation index (d value) of heterozygosis was-1.000 in the two populations. Ho of Est in T. brandti population was 0.900, and d value was 0.818. Remarked deviations from Hardy-Weinberg equilibrium were observed at the loci of Ldh-1 , a-Gpd-1 , Sdh-1 and Aat-1 in the two species of fishes except Gpi-1 in T. brandti and Est in T. hakonensis. Sig- nificant heterozygote absent was observed with Ldh-1 , α-Gpd-1, Sdh-1 and Aat-1 , as well as the homozy- gote excess with Est in T. brandti and T. hakonensis. The genetic distance and genetic similarity between the two breeding fishes was 0.369 3 and 0.691 2. The genetic diversity levels of the two fishes were up to species level.
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