Huang, Yan
(China Research and Conservation Center for the Giant Panda)
,
Wang, Pengyan
(China Research and Conservation Center for the Giant Panda)
,
Rebecca, E Spindler
(Conservation & Research Center, Smithsonian National Zoological Park)
,
Jogayle, Howard
(Conservation & Research Center, Smithsonian National Zoological Park)
,
Zhang, Hemin
(China Research and Conservation Center for the Giant Panda)
,
David, E Wildt
(Conservation & Research Center, Smithsonian National Zoological Park)
A management priority for giant pandas is developing cryopreserved sperm banks to use in conjunction with artificial insemination to protect extant diversity and transport genetic material between geographically disparate panda populations. To be an effective tool, these kinks, sperm viability post-...
A management priority for giant pandas is developing cryopreserved sperm banks to use in conjunction with artificial insemination to protect extant diversity and transport genetic material between geographically disparate panda populations. To be an effective tool, these kinks, sperm viability post-thaw must be optimized. These studies examined the influence of 1) different thaw rates and 2) the chemical stimulant pentyoxyfilline (PF) , on sperm viability and motility cryopreserved at rapid rates ( - 40 to - 100℃/min). Semen was collected from 11 adult giant pandas and cryopreserved rapidly ( - 40 to - 100 ℃/min) . In study I , sperm was thawed at various rates by plunging into water baths at (1) 22℃; (slow rate thawing); (2) 37℃ (intermediate thaw rate); or (3) 50℃ (rapid thaw rate). When compared to mean pre-freeze giant panda sperm motility (78.1 ± 2.9% ) , post-thaw motility decreased (P 0.05) sperm motility, progressive status, viability and aerosomal integrity patterns throughout the 90 min examination period.Tang-term incubation with 1 mM PF, did not influence motility among time periods (P > 0.05) . Giant panda spenn incubated with 5mM and 10 mM PF were less ( P < 0.05) motile by 4 hrs (5 mM: 24.0 ± 4.7; 10 mM 19.5 ± 3.6%) compared with controls (38.3 ± 5.2%). Additionally, spenn incubated with 10 mM PF had fewer (P<0.05) intact acrosomes (56.3 ± 2.8) after 4 hrs incubation, compared with controls (71.0 ± 3.3%) . These two studies of giant panda spenn demonstrated that giant panda sperm that are cooled and cryopreserved rapidly in pellet form, have better survival following slow rate thaw. Further, giant panda sperm are sensitive to the common motility enhancer PF. However, this boost is short-term, actually depressing spenn motility after a few hours of incubation and, at higher concentrations, damaging acrosomal integrity.
A management priority for giant pandas is developing cryopreserved sperm banks to use in conjunction with artificial insemination to protect extant diversity and transport genetic material between geographically disparate panda populations. To be an effective tool, these kinks, sperm viability post-thaw must be optimized. These studies examined the influence of 1) different thaw rates and 2) the chemical stimulant pentyoxyfilline (PF) , on sperm viability and motility cryopreserved at rapid rates ( - 40 to - 100℃/min). Semen was collected from 11 adult giant pandas and cryopreserved rapidly ( - 40 to - 100 ℃/min) . In study I , sperm was thawed at various rates by plunging into water baths at (1) 22℃; (slow rate thawing); (2) 37℃ (intermediate thaw rate); or (3) 50℃ (rapid thaw rate). When compared to mean pre-freeze giant panda sperm motility (78.1 ± 2.9% ) , post-thaw motility decreased (P 0.05) sperm motility, progressive status, viability and aerosomal integrity patterns throughout the 90 min examination period.Tang-term incubation with 1 mM PF, did not influence motility among time periods (P > 0.05) . Giant panda spenn incubated with 5mM and 10 mM PF were less ( P < 0.05) motile by 4 hrs (5 mM: 24.0 ± 4.7; 10 mM 19.5 ± 3.6%) compared with controls (38.3 ± 5.2%). Additionally, spenn incubated with 10 mM PF had fewer (P<0.05) intact acrosomes (56.3 ± 2.8) after 4 hrs incubation, compared with controls (71.0 ± 3.3%) . These two studies of giant panda spenn demonstrated that giant panda sperm that are cooled and cryopreserved rapidly in pellet form, have better survival following slow rate thaw. Further, giant panda sperm are sensitive to the common motility enhancer PF. However, this boost is short-term, actually depressing spenn motility after a few hours of incubation and, at higher concentrations, damaging acrosomal integrity.
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