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To clarify the effect of Cornus fructus (CF) extract on the cultured NIH3T3 fibroblasts injured by hydrogen peroxide (H₂O₂), this study was carried out 새 examine the protective effect of cell adhesion ability and oxidative stress of H₂O₂ in cultured NIH3T3 fibroblasts. For this study, XTT assay for ...

To clarify the effect of Cornus fructus (CF) extract on the cultured NIH3T3 fibroblasts injured by hydrogen peroxide (H₂O₂), this study was carried out 새 examine the protective effect of cell adhesion ability and oxidative stress of H₂O₂ in cultured NIH3T3 fibroblasts. For this study, XTT assay for the cell adhesion ability, lactate dehydrogenase (LDH) activity and suproxide dismutase (SOD)-like activity for oxidative stress of H₂O₂ were done in the cultured NIH3T3 fibroblasts, after NIH3T3 fibroblasts were incubated with various concentrations of H₂O₂ or CF extract. In this study, H₂O₂ decreased the cell adhesion ability of NIH3T3 fibroblasts in dose- and time-dependent manners. And XTT₅₀ value was determined at 25uM of H2O2 which was highly toxic by the criteria of cytotoxicity. In the effect of CF exreact on the H₂O₂-induced cytotoxicity, CF extract significantly increased cell adhesion ability injured by the cytotoxicity of H₂O₂ in cultured NIH3T3 fibroblasts. CF extract also deceased LDH activity which was increased by the H₂O₂-induced cytotoxicity, and showed SOD-like activity. From above the results, it is suggested that H2O2 was highly toxic on cultured NIH3T3 fibroblasts, and also, CF extract showed the protective effect on oxidative stress of H₂O₂ by the showing of SOD-like activity and the decrease of LDH activity.

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